Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells
<p>Abstract</p> <p>Background</p> <p>Recently, periostin (<it>POSTN</it>), a gene encoding a protein with similarity to the fasciclin family and involved in cell survival and angiogenesis, has emerged as a promising marker for tumor progression in various ty...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-12-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/6/1/80 |
| _version_ | 1811252011181539328 |
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| author | van Baren Nicolas Brasseur Francis Mattiussi Marina Tilman Gaëlle Decottignies Anabelle |
| author_facet | van Baren Nicolas Brasseur Francis Mattiussi Marina Tilman Gaëlle Decottignies Anabelle |
| author_sort | van Baren Nicolas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recently, periostin (<it>POSTN</it>), a gene encoding a protein with similarity to the fasciclin family and involved in cell survival and angiogenesis, has emerged as a promising marker for tumor progression in various types of human cancers. There is some controversy regarding both <it>POSTN </it>expression levels and the nature of periostin-producing cells within tumors. In this study, we used quantitative RT-PCR to assess periostin gene expression in normal tissues, primary cell cultures, tumor tissues and tumor cell lines.</p> <p>Results</p> <p>Periostin expression levels are highly variable in both normal tissues and tumors and strong <it>POSTN </it>overexpression is mostly detected in tumors from pancreas and liver. <it>POSTN </it>is not expressed in blood cancers. In melanoma samples, average periostin expression is not increased in primary tumors whereas <it>POSTN </it>overexpression was detected in about 60% of melanoma metastatic tumors in the liver or lymph nodes. Identification of the cellular source of periostin production in melanoma metastases -cancer cells or stroma- was assessed by comparing periostin expression in 23 newly-established melanoma cell lines and matched tumors. In contrast to the reduction by more than 99% of <it>COL6A3 </it>stromal marker mRNA in all cell lines, significant <it>POSTN </it>transcription was maintained in some melanoma cell lines, suggesting that both stromal cells and melanoma cells express periostin. The high level of periostin expression in primary cultures of skin fibroblasts suggests that fibroblasts may contribute for a large part to periostin production in melanoma-associated stroma. On the other hand, periostin expression in melanoma cells is probably acquired during the tumorigenic process as 1) normal melanocytes do not express <it>POSTN </it>and 2) melanoma cells from distinct metastases of the same patient were associated with very different levels of periostin expression.</p> <p>Conclusion</p> <p>Our comparative analysis suggests that, although periostin overexpression is clearly detected in some cancers, it is not a general feature of tumors. In melanoma, our study identifies both stromal and melanoma cells as sources of periostin production and correlates <it>POSTN </it>expression levels with increased primary tumor thickness and metastatic process development.</p> |
| first_indexed | 2024-04-12T16:28:29Z |
| format | Article |
| id | doaj.art-97fc8281de2042f8b00be5f1d68278af |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-12T16:28:29Z |
| publishDate | 2007-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-97fc8281de2042f8b00be5f1d68278af2022-12-22T03:25:16ZengBMCMolecular Cancer1476-45982007-12-01618010.1186/1476-4598-6-80Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cellsvan Baren NicolasBrasseur FrancisMattiussi MarinaTilman GaëlleDecottignies Anabelle<p>Abstract</p> <p>Background</p> <p>Recently, periostin (<it>POSTN</it>), a gene encoding a protein with similarity to the fasciclin family and involved in cell survival and angiogenesis, has emerged as a promising marker for tumor progression in various types of human cancers. There is some controversy regarding both <it>POSTN </it>expression levels and the nature of periostin-producing cells within tumors. In this study, we used quantitative RT-PCR to assess periostin gene expression in normal tissues, primary cell cultures, tumor tissues and tumor cell lines.</p> <p>Results</p> <p>Periostin expression levels are highly variable in both normal tissues and tumors and strong <it>POSTN </it>overexpression is mostly detected in tumors from pancreas and liver. <it>POSTN </it>is not expressed in blood cancers. In melanoma samples, average periostin expression is not increased in primary tumors whereas <it>POSTN </it>overexpression was detected in about 60% of melanoma metastatic tumors in the liver or lymph nodes. Identification of the cellular source of periostin production in melanoma metastases -cancer cells or stroma- was assessed by comparing periostin expression in 23 newly-established melanoma cell lines and matched tumors. In contrast to the reduction by more than 99% of <it>COL6A3 </it>stromal marker mRNA in all cell lines, significant <it>POSTN </it>transcription was maintained in some melanoma cell lines, suggesting that both stromal cells and melanoma cells express periostin. The high level of periostin expression in primary cultures of skin fibroblasts suggests that fibroblasts may contribute for a large part to periostin production in melanoma-associated stroma. On the other hand, periostin expression in melanoma cells is probably acquired during the tumorigenic process as 1) normal melanocytes do not express <it>POSTN </it>and 2) melanoma cells from distinct metastases of the same patient were associated with very different levels of periostin expression.</p> <p>Conclusion</p> <p>Our comparative analysis suggests that, although periostin overexpression is clearly detected in some cancers, it is not a general feature of tumors. In melanoma, our study identifies both stromal and melanoma cells as sources of periostin production and correlates <it>POSTN </it>expression levels with increased primary tumor thickness and metastatic process development.</p>http://www.molecular-cancer.com/content/6/1/80 |
| spellingShingle | van Baren Nicolas Brasseur Francis Mattiussi Marina Tilman Gaëlle Decottignies Anabelle Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells Molecular Cancer |
| title | Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells |
| title_full | Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells |
| title_fullStr | Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells |
| title_full_unstemmed | Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells |
| title_short | Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells |
| title_sort | human periostin gene expression in normal tissues tumors and melanoma evidences for periostin production by both stromal and melanoma cells |
| url | http://www.molecular-cancer.com/content/6/1/80 |
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