Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study
Introduction: Ultrasonic scaling involves the production of a high amount of aerosols, which combine with oral microbes and become a potential source of airborne contaminants. Chemical antimicrobial agents like Chlorhexidine (CHX) (the gold standard) are known to reduce microbial contamination...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2023-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/18720/64913_CE[Ra1]_F(IS)_QC(KK_RDW_IS)_PF1(RI_OM)_PFA(RI_KM)_PN(KM).pdf |
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| author | R M Vasanthi Roshan R Rughwani Priyanka K Cholan Dhayanand John Victor S Savithri |
| author_facet | R M Vasanthi Roshan R Rughwani Priyanka K Cholan Dhayanand John Victor S Savithri |
| author_sort | R M Vasanthi |
| collection | DOAJ |
| description | Introduction: Ultrasonic scaling involves the production of a
high amount of aerosols, which combine with oral microbes
and become a potential source of airborne contaminants.
Chemical antimicrobial agents like Chlorhexidine (CHX) (the
gold standard) are known to reduce microbial contamination
in dental waterlines. However, natural extracts are gaining
popularity as an economical yet effective means of reducing
bacterial load.
Aim: To compare the efficacy of neem and cinnamon extracts in
reducing bacterial contamination during scaling.
Materials and Methods: A single-centered, parallel multiplearm, randomised clinical study was conducted at the Department
of Periodontics and Oral Implantology, SRM Dental College,
Ramapuram, Chennai, Tamil Nadu, India from September 2019
to March 2020. Scaling was performed for patients in all four
groups (n=128, 32 each group) - Water, 0.2% Chlorhexidine,
Neem, and Cinnamon Extract. Two blood agar petri dishes were
placed on either side of the patient at a distance of 40 cm from
the patient’s head, and one blood agar petri dish was placed
on the operator’s chest. The agar plates were then incubated
aerobically for 48 hours for Colony Forming Units (CFU) count.
Statistical analysis was performed using one-way Analysis of
Variance (ANOVA) and Tukey’s posthoc test.
Results: Cinnamon showed a 25.2% reduction in CFUs,
whereas neem showed an 11.7% reduction compared to
distilled water. However, chlorhexidine had the maximum
bacterial reduction. The agar plates at the chest position
had the highest bacterial growth, while the left position had
comparatively fewer microbial colonies.
Conclusion: Chlorhexidine exhibited the highest percentage
of CFU reduction. However, when used as a Dental Unit Water
Line (DUWL) coolant, herbal extracts such as cinnamon and
neem caused a significant reduction in CFUs compared to
distilled water, with cinnamon performing better than neem.
Therefore, herbal extracts like neem and cinnamon can be
considered cost-effective alternatives for minimising aerosol
contamination in DUWL. |
| first_indexed | 2024-03-09T14:11:01Z |
| format | Article |
| id | doaj.art-97fde151289d49929da48522f5fe3ab5 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-03-09T14:11:01Z |
| publishDate | 2023-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-97fde151289d49929da48522f5fe3ab52023-11-29T11:10:04ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2023-11-011711162010.7860/JCDR/2023/64913.18720Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical StudyR M Vasanthi0Roshan R Rughwani1Priyanka K Cholan2Dhayanand John Victor3S Savithri4Postgraduate Student, Department of Periodontics, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India.Postgraduate Student, Department of Periodontics, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India.Associate Professor, Department of Periodontics, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India.Professor and Head, Department of Periodontics, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India.Professor and Head, Department of Microbiology, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India.Introduction: Ultrasonic scaling involves the production of a high amount of aerosols, which combine with oral microbes and become a potential source of airborne contaminants. Chemical antimicrobial agents like Chlorhexidine (CHX) (the gold standard) are known to reduce microbial contamination in dental waterlines. However, natural extracts are gaining popularity as an economical yet effective means of reducing bacterial load. Aim: To compare the efficacy of neem and cinnamon extracts in reducing bacterial contamination during scaling. Materials and Methods: A single-centered, parallel multiplearm, randomised clinical study was conducted at the Department of Periodontics and Oral Implantology, SRM Dental College, Ramapuram, Chennai, Tamil Nadu, India from September 2019 to March 2020. Scaling was performed for patients in all four groups (n=128, 32 each group) - Water, 0.2% Chlorhexidine, Neem, and Cinnamon Extract. Two blood agar petri dishes were placed on either side of the patient at a distance of 40 cm from the patient’s head, and one blood agar petri dish was placed on the operator’s chest. The agar plates were then incubated aerobically for 48 hours for Colony Forming Units (CFU) count. Statistical analysis was performed using one-way Analysis of Variance (ANOVA) and Tukey’s posthoc test. Results: Cinnamon showed a 25.2% reduction in CFUs, whereas neem showed an 11.7% reduction compared to distilled water. However, chlorhexidine had the maximum bacterial reduction. The agar plates at the chest position had the highest bacterial growth, while the left position had comparatively fewer microbial colonies. Conclusion: Chlorhexidine exhibited the highest percentage of CFU reduction. However, when used as a Dental Unit Water Line (DUWL) coolant, herbal extracts such as cinnamon and neem caused a significant reduction in CFUs compared to distilled water, with cinnamon performing better than neem. Therefore, herbal extracts like neem and cinnamon can be considered cost-effective alternatives for minimising aerosol contamination in DUWL.https://www.jcdr.net/articles/PDF/18720/64913_CE[Ra1]_F(IS)_QC(KK_RDW_IS)_PF1(RI_OM)_PFA(RI_KM)_PN(KM).pdfaerosol contaminationdental unit water linesherbal extracts |
| spellingShingle | R M Vasanthi Roshan R Rughwani Priyanka K Cholan Dhayanand John Victor S Savithri Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study Journal of Clinical and Diagnostic Research aerosol contamination dental unit water lines herbal extracts |
| title | Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study |
| title_full | Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study |
| title_fullStr | Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study |
| title_full_unstemmed | Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study |
| title_short | Efficacy of Neem and Cinnamon Extracts in Reducing Bacterial Contamination during Scaling: A Randomised Clinical Study |
| title_sort | efficacy of neem and cinnamon extracts in reducing bacterial contamination during scaling a randomised clinical study |
| topic | aerosol contamination dental unit water lines herbal extracts |
| url | https://www.jcdr.net/articles/PDF/18720/64913_CE[Ra1]_F(IS)_QC(KK_RDW_IS)_PF1(RI_OM)_PFA(RI_KM)_PN(KM).pdf |
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