<i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line
Endometriosis is a common gynecological disorder characterized by ectopic growth of endometrium outside the uterus and is associated with chronic pain and infertility. We investigated the role of the long intergenic noncoding RNA 01133 (<i>LINC01133</i>) in endometriosis, an lncRNA that...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/16/8385 |
| _version_ | 1797523649492680704 |
|---|---|
| author | Iveta Yotova Quanah J. Hudson Florian M. Pauler Katharina Proestling Isabella Haslinger Lorenz Kuessel Alexandra Perricos Heinrich Husslein René Wenzl |
| author_facet | Iveta Yotova Quanah J. Hudson Florian M. Pauler Katharina Proestling Isabella Haslinger Lorenz Kuessel Alexandra Perricos Heinrich Husslein René Wenzl |
| author_sort | Iveta Yotova |
| collection | DOAJ |
| description | Endometriosis is a common gynecological disorder characterized by ectopic growth of endometrium outside the uterus and is associated with chronic pain and infertility. We investigated the role of the long intergenic noncoding RNA 01133 (<i>LINC01133</i>) in endometriosis, an lncRNA that has been implicated in several types of cancer. We found that <i>LINC01133</i> is upregulated in ectopic endometriotic lesions. As expression appeared higher in the epithelial endometrial layer, we performed a siRNA knockdown of <i>LINC01133</i> in an endometriosis epithelial cell line. Phenotypic assays indicated that <i>LINC01133</i> may promote proliferation and suppress cellular migration, and affect the cytoskeleton and morphology of the cells. Gene ontology analysis of differentially expressed genes indicated that cell proliferation and migration pathways were affected in line with the observed phenotype. We validated upregulation of p21 and downregulation of Cyclin A at the protein level, which together with the quantification of the DNA content using fluorescence-activated cell sorting (FACS) analysis indicated that the observed effects on cellular proliferation may be due to changes in cell cycle. Further, we found testis-specific protein kinase 1 (TESK1) kinase upregulation corresponding with phosphorylation and inactivation of actin severing protein Cofilin, which could explain changes in the cytoskeleton and cellular migration. These results indicate that endometriosis is associated with <i>LINC01133</i> upregulation, which may affect pathogenesis via the cellular proliferation and migration pathways. |
| first_indexed | 2024-03-10T08:46:03Z |
| format | Article |
| id | doaj.art-97fde8b6ed9e4a82846623e8b7cf4c77 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:46:03Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-97fde8b6ed9e4a82846623e8b7cf4c772023-11-22T07:54:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216838510.3390/ijms22168385<i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell LineIveta Yotova0Quanah J. Hudson1Florian M. Pauler2Katharina Proestling3Isabella Haslinger4Lorenz Kuessel5Alexandra Perricos6Heinrich Husslein7René Wenzl8Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaDepartment of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, AustriaEndometriosis is a common gynecological disorder characterized by ectopic growth of endometrium outside the uterus and is associated with chronic pain and infertility. We investigated the role of the long intergenic noncoding RNA 01133 (<i>LINC01133</i>) in endometriosis, an lncRNA that has been implicated in several types of cancer. We found that <i>LINC01133</i> is upregulated in ectopic endometriotic lesions. As expression appeared higher in the epithelial endometrial layer, we performed a siRNA knockdown of <i>LINC01133</i> in an endometriosis epithelial cell line. Phenotypic assays indicated that <i>LINC01133</i> may promote proliferation and suppress cellular migration, and affect the cytoskeleton and morphology of the cells. Gene ontology analysis of differentially expressed genes indicated that cell proliferation and migration pathways were affected in line with the observed phenotype. We validated upregulation of p21 and downregulation of Cyclin A at the protein level, which together with the quantification of the DNA content using fluorescence-activated cell sorting (FACS) analysis indicated that the observed effects on cellular proliferation may be due to changes in cell cycle. Further, we found testis-specific protein kinase 1 (TESK1) kinase upregulation corresponding with phosphorylation and inactivation of actin severing protein Cofilin, which could explain changes in the cytoskeleton and cellular migration. These results indicate that endometriosis is associated with <i>LINC01133</i> upregulation, which may affect pathogenesis via the cellular proliferation and migration pathways.https://www.mdpi.com/1422-0067/22/16/8385endometriosislong noncoding RNAslncRNAsepithelial to mesenchymal transitionEMTproliferation |
| spellingShingle | Iveta Yotova Quanah J. Hudson Florian M. Pauler Katharina Proestling Isabella Haslinger Lorenz Kuessel Alexandra Perricos Heinrich Husslein René Wenzl <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line International Journal of Molecular Sciences endometriosis long noncoding RNAs lncRNAs epithelial to mesenchymal transition EMT proliferation |
| title | <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line |
| title_full | <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line |
| title_fullStr | <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line |
| title_full_unstemmed | <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line |
| title_short | <i>LINC01133</i> Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line |
| title_sort | i linc01133 i inhibits invasion and promotes proliferation in an endometriosis epithelial cell line |
| topic | endometriosis long noncoding RNAs lncRNAs epithelial to mesenchymal transition EMT proliferation |
| url | https://www.mdpi.com/1422-0067/22/16/8385 |
| work_keys_str_mv | AT ivetayotova ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT quanahjhudson ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT florianmpauler ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT katharinaproestling ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT isabellahaslinger ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT lorenzkuessel ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT alexandraperricos ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT heinrichhusslein ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline AT renewenzl ilinc01133iinhibitsinvasionandpromotesproliferationinanendometriosisepithelialcellline |