Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia

Abstract Ischemic stroke is one of the most severe polygenic brain diseases. Here, we performed further functional genetic analysis of the processes occurring in the contralateral hemisphere (CH) after ischemia–reperfusion injury in rat brain. Comparison of RNA sequencing data for subcortical sample...

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Main Authors: Ivan B. Filippenkov, Julia A. Remizova, Alina E. Denisova, Vasily V. Stavchansky, Ksenia D. Golovina, Leonid V. Gubsky, Svetlana A. Limborska, Lyudmila V. Dergunova
Format: Article
Language:English
Published: Nature Portfolio 2023-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-27663-8
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author Ivan B. Filippenkov
Julia A. Remizova
Alina E. Denisova
Vasily V. Stavchansky
Ksenia D. Golovina
Leonid V. Gubsky
Svetlana A. Limborska
Lyudmila V. Dergunova
author_facet Ivan B. Filippenkov
Julia A. Remizova
Alina E. Denisova
Vasily V. Stavchansky
Ksenia D. Golovina
Leonid V. Gubsky
Svetlana A. Limborska
Lyudmila V. Dergunova
author_sort Ivan B. Filippenkov
collection DOAJ
description Abstract Ischemic stroke is one of the most severe polygenic brain diseases. Here, we performed further functional genetic analysis of the processes occurring in the contralateral hemisphere (CH) after ischemia–reperfusion injury in rat brain. Comparison of RNA sequencing data for subcortical samples from the ipsilateral hemisphere (IH) and CH after 90 min of transient middle cerebral artery occlusion (tMCAO) and corresponding sham-operated (SO) controls showed four groups of genes that were associated with ischemic processes in rat brain at 24 h after tMCAO. Among them, 2672 genes were differentially expressed genes (DEGs) for IH but non-DEGs for CH, 34 genes were DEGs for CH but non-DEGs for IH, and 114 genes had codirected changes in expression in both hemispheres. The remaining 16 genes exhibited opposite changes at the mRNA level in the two brain hemispheres after tMCAO. These findings suggest that the ischemic process caused by a focal ischemia induces complex bilateral reactions at the transcriptome level in the rat brain. We believe that specific genome responses in the CH and IH may provide a useful model for the study of the potential for brain repair after stroke.
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spelling doaj.art-97ff7a1652614317b2bd0032f0d1a6ec2023-01-15T12:08:26ZengNature PortfolioScientific Reports2045-23222023-01-0113111110.1038/s41598-023-27663-8Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemiaIvan B. Filippenkov0Julia A. Remizova1Alina E. Denisova2Vasily V. Stavchansky3Ksenia D. Golovina4Leonid V. Gubsky5Svetlana A. Limborska6Lyudmila V. Dergunova7Institute of Molecular Genetics of National Research Center “Kurchatov Institute”Institute of Molecular Genetics of National Research Center “Kurchatov Institute”Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical UniversityInstitute of Molecular Genetics of National Research Center “Kurchatov Institute”Institute of Molecular Genetics of National Research Center “Kurchatov Institute”Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical UniversityInstitute of Molecular Genetics of National Research Center “Kurchatov Institute”Institute of Molecular Genetics of National Research Center “Kurchatov Institute”Abstract Ischemic stroke is one of the most severe polygenic brain diseases. Here, we performed further functional genetic analysis of the processes occurring in the contralateral hemisphere (CH) after ischemia–reperfusion injury in rat brain. Comparison of RNA sequencing data for subcortical samples from the ipsilateral hemisphere (IH) and CH after 90 min of transient middle cerebral artery occlusion (tMCAO) and corresponding sham-operated (SO) controls showed four groups of genes that were associated with ischemic processes in rat brain at 24 h after tMCAO. Among them, 2672 genes were differentially expressed genes (DEGs) for IH but non-DEGs for CH, 34 genes were DEGs for CH but non-DEGs for IH, and 114 genes had codirected changes in expression in both hemispheres. The remaining 16 genes exhibited opposite changes at the mRNA level in the two brain hemispheres after tMCAO. These findings suggest that the ischemic process caused by a focal ischemia induces complex bilateral reactions at the transcriptome level in the rat brain. We believe that specific genome responses in the CH and IH may provide a useful model for the study of the potential for brain repair after stroke.https://doi.org/10.1038/s41598-023-27663-8
spellingShingle Ivan B. Filippenkov
Julia A. Remizova
Alina E. Denisova
Vasily V. Stavchansky
Ksenia D. Golovina
Leonid V. Gubsky
Svetlana A. Limborska
Lyudmila V. Dergunova
Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
Scientific Reports
title Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
title_full Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
title_fullStr Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
title_full_unstemmed Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
title_short Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
title_sort differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
url https://doi.org/10.1038/s41598-023-27663-8
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