Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM)
In this report, we propose a micro vacuum chuck (MVC) which can connect three-dimensional (3D) tissues to a tensile test system by vacuum pressure. Because the MVC fixes the 3D tissue by vacuum pressure generated on multiple vacuum holes, it is expected that the MVC can fix 3D tissue to the system e...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-07-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/10/7/487 |
| _version_ | 1819084006421954560 |
|---|---|
| author | Kaoru Uesugi Fumiaki Shima Ken Fukumoto Ayami Hiura Yoshinari Tsukamoto Shigeru Miyagawa Yoshiki Sawa Takami Akagi Mitsuru Akashi Keisuke Morishima |
| author_facet | Kaoru Uesugi Fumiaki Shima Ken Fukumoto Ayami Hiura Yoshinari Tsukamoto Shigeru Miyagawa Yoshiki Sawa Takami Akagi Mitsuru Akashi Keisuke Morishima |
| author_sort | Kaoru Uesugi |
| collection | DOAJ |
| description | In this report, we propose a micro vacuum chuck (MVC) which can connect three-dimensional (3D) tissues to a tensile test system by vacuum pressure. Because the MVC fixes the 3D tissue by vacuum pressure generated on multiple vacuum holes, it is expected that the MVC can fix 3D tissue to the system easily and mitigate the damage which can happen by handling during fixing. In order to decide optimum conditions for the size of the vacuum holes and the vacuum pressure, various sized vacuum holes and vacuum pressures were applied to a normal human cardiac fibroblast 3D tissue. From the results, we confirmed that a square shape with 100 µm sides was better for fixing the 3D tissue. Then we mounted our developed MVCs on a specially developed tensile test system and measured the bio-mechanical property (beating force) of cardiac 3D tissue which was constructed of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CM); the 3D tissue had been assembled by the layer-by-layer (LbL) method. We measured the beating force of the cardiac 3D tissue and confirmed the measured force followed the Frank-Starling relationship. This indicates that the beating property of cardiac 3D tissue obtained by the LbL method was close to that of native cardiac tissue. |
| first_indexed | 2024-12-21T20:41:36Z |
| format | Article |
| id | doaj.art-98003e07d2a14f4d8111f9d682ae762c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-21T20:41:36Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-98003e07d2a14f4d8111f9d682ae762c2022-12-21T18:50:57ZengMDPI AGMicromachines2072-666X2019-07-0110748710.3390/mi10070487mi10070487Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM)Kaoru Uesugi0Fumiaki Shima1Ken Fukumoto2Ayami Hiura3Yoshinari Tsukamoto4Shigeru Miyagawa5Yoshiki Sawa6Takami Akagi7Mitsuru Akashi8Keisuke Morishima9Department of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, JapanBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, JapanBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, JapanBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, JapanBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, JapanGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, JapanGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, JapanBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, JapanGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, JapanDepartment of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, JapanIn this report, we propose a micro vacuum chuck (MVC) which can connect three-dimensional (3D) tissues to a tensile test system by vacuum pressure. Because the MVC fixes the 3D tissue by vacuum pressure generated on multiple vacuum holes, it is expected that the MVC can fix 3D tissue to the system easily and mitigate the damage which can happen by handling during fixing. In order to decide optimum conditions for the size of the vacuum holes and the vacuum pressure, various sized vacuum holes and vacuum pressures were applied to a normal human cardiac fibroblast 3D tissue. From the results, we confirmed that a square shape with 100 µm sides was better for fixing the 3D tissue. Then we mounted our developed MVCs on a specially developed tensile test system and measured the bio-mechanical property (beating force) of cardiac 3D tissue which was constructed of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CM); the 3D tissue had been assembled by the layer-by-layer (LbL) method. We measured the beating force of the cardiac 3D tissue and confirmed the measured force followed the Frank-Starling relationship. This indicates that the beating property of cardiac 3D tissue obtained by the LbL method was close to that of native cardiac tissue.https://www.mdpi.com/2072-666X/10/7/487beating forcebio-mechanical propertycardiac 3D tissuehuman induced pluripotent Stem cell-derived cardiomyocytes (hiPS-CM)tissue engineeringvacuum chuck |
| spellingShingle | Kaoru Uesugi Fumiaki Shima Ken Fukumoto Ayami Hiura Yoshinari Tsukamoto Shigeru Miyagawa Yoshiki Sawa Takami Akagi Mitsuru Akashi Keisuke Morishima Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) Micromachines beating force bio-mechanical property cardiac 3D tissue human induced pluripotent Stem cell-derived cardiomyocytes (hiPS-CM) tissue engineering vacuum chuck |
| title | Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) |
| title_full | Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) |
| title_fullStr | Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) |
| title_full_unstemmed | Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) |
| title_short | Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM) |
| title_sort | micro vacuum chuck and tensile test system for bio mechanical evaluation of 3d tissue constructed of human induced pluripotent stem cell derived cardiomyocytes hips cm |
| topic | beating force bio-mechanical property cardiac 3D tissue human induced pluripotent Stem cell-derived cardiomyocytes (hiPS-CM) tissue engineering vacuum chuck |
| url | https://www.mdpi.com/2072-666X/10/7/487 |
| work_keys_str_mv | AT kaoruuesugi microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT fumiakishima microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT kenfukumoto microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT ayamihiura microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT yoshinaritsukamoto microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT shigerumiyagawa microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT yoshikisawa microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT takamiakagi microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT mitsuruakashi microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm AT keisukemorishima microvacuumchuckandtensiletestsystemforbiomechanicalevaluationof3dtissueconstructedofhumaninducedpluripotentstemcellderivedcardiomyocyteshipscm |