PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients
OBJECTIVES: The phosphatidylinositol 3-kinase/AKT axis is an important cell-signaling pathway that mediates cell proliferation and survival, two biological processes that regulate malignant cell growth. The phosphatidylinositol 3-kinase CA gene encodes the p110α subunit of the phosphatidylinositol...
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| Format: | Article |
| Language: | English |
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Elsevier España
2012-11-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012001100011 |
| _version_ | 1817978251656560640 |
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| author | Flavia R. Mangone Irina G. Bobrovnitchaia Sibeli Salaorni Erika Manuli Maria A. Nagai |
| author_facet | Flavia R. Mangone Irina G. Bobrovnitchaia Sibeli Salaorni Erika Manuli Maria A. Nagai |
| author_sort | Flavia R. Mangone |
| collection | DOAJ |
| description | OBJECTIVES: The phosphatidylinositol 3-kinase/AKT axis is an important cell-signaling pathway that mediates cell proliferation and survival, two biological processes that regulate malignant cell growth. The phosphatidylinositol 3-kinase CA gene encodes the p110α subunit of the phosphatidylinositol 3-kinase protein. There are phosphatidylinositol 3-kinase CA mutations in several types of human tumors, and they are frequently observed in breast cancer. However, these mutations have not been investigated in Brazilian breast cancer patients. METHODS: PCR-SSCP and direct DNA sequencing were performed to identify phosphatidylinositol 3-kinaseCA exon 9 and exon 20 mutations in 86 patients with sporadic breast cancer. The relationships between PIK3CA mutations and patient clinicopathological characteristics and survival were analyzed. The presence of the TP53 mutation was also examined. RESULTS: Twenty-three (27%) of the 86 primary breast tumors contained PIK3CA mutations. In exons 9 and 20, we identified the hotspot mutations E542K, E545K, and H1047R, and we identified two new missense mutations (I1022V and L1028S) and one nonsense (R992X) mutation. Phosphatidylinositol 3-kinase CA exon 20 mutations were associated with poor overall survival and TP53 gene mutations. CONCLUSIONS: Phosphatidylinositol 3-kinase CA mutations are common in tumors in Brazilian breast cancer patients, and phosphatidylinositol 3-kinase CA and TP53 mutations are not mutually exclusive. Phosphatidylinositol 3-kinase CA exon 20 mutations are associated with poor survival, and they may be useful biomarkers for identifying breast cancer patients with aggressive tumors and for predicting the response to treatment with PI3K pathway inhibitors. |
| first_indexed | 2024-04-13T22:27:00Z |
| format | Article |
| id | doaj.art-9800bb5d8e12447798ac4b9a8bb9bdb0 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T22:27:00Z |
| publishDate | 2012-11-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-9800bb5d8e12447798ac4b9a8bb9bdb02022-12-22T02:27:02ZengElsevier EspañaClinics1807-59321980-53222012-11-0167111285129010.6061/clinics/2012(11)11PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patientsFlavia R. MangoneIrina G. BobrovnitchaiaSibeli SalaorniErika ManuliMaria A. NagaiOBJECTIVES: The phosphatidylinositol 3-kinase/AKT axis is an important cell-signaling pathway that mediates cell proliferation and survival, two biological processes that regulate malignant cell growth. The phosphatidylinositol 3-kinase CA gene encodes the p110α subunit of the phosphatidylinositol 3-kinase protein. There are phosphatidylinositol 3-kinase CA mutations in several types of human tumors, and they are frequently observed in breast cancer. However, these mutations have not been investigated in Brazilian breast cancer patients. METHODS: PCR-SSCP and direct DNA sequencing were performed to identify phosphatidylinositol 3-kinaseCA exon 9 and exon 20 mutations in 86 patients with sporadic breast cancer. The relationships between PIK3CA mutations and patient clinicopathological characteristics and survival were analyzed. The presence of the TP53 mutation was also examined. RESULTS: Twenty-three (27%) of the 86 primary breast tumors contained PIK3CA mutations. In exons 9 and 20, we identified the hotspot mutations E542K, E545K, and H1047R, and we identified two new missense mutations (I1022V and L1028S) and one nonsense (R992X) mutation. Phosphatidylinositol 3-kinase CA exon 20 mutations were associated with poor overall survival and TP53 gene mutations. CONCLUSIONS: Phosphatidylinositol 3-kinase CA mutations are common in tumors in Brazilian breast cancer patients, and phosphatidylinositol 3-kinase CA and TP53 mutations are not mutually exclusive. Phosphatidylinositol 3-kinase CA exon 20 mutations are associated with poor survival, and they may be useful biomarkers for identifying breast cancer patients with aggressive tumors and for predicting the response to treatment with PI3K pathway inhibitors.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012001100011Breast NeoplasmPIK3CATP53MutationPrognosis |
| spellingShingle | Flavia R. Mangone Irina G. Bobrovnitchaia Sibeli Salaorni Erika Manuli Maria A. Nagai PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients Clinics Breast Neoplasm PIK3CA TP53 Mutation Prognosis |
| title | PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients |
| title_full | PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients |
| title_fullStr | PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients |
| title_full_unstemmed | PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients |
| title_short | PIK3CA exon 20 mutations are associated with poor prognosis in breast cancer patients |
| title_sort | pik3ca exon 20 mutations are associated with poor prognosis in breast cancer patients |
| topic | Breast Neoplasm PIK3CA TP53 Mutation Prognosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012001100011 |
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