Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly
Abstract Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which repr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-05-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-37915-w |
| _version_ | 1797827453960323072 |
|---|---|
| author | Rebecca Conners Rayén Ignacia León-Quezada Mathew McLaren Nicholas J. Bennett Bertram Daum Jasna Rakonjac Vicki A. M. Gold |
| author_facet | Rebecca Conners Rayén Ignacia León-Quezada Mathew McLaren Nicholas J. Bennett Bertram Daum Jasna Rakonjac Vicki A. M. Gold |
| author_sort | Rebecca Conners |
| collection | DOAJ |
| description | Abstract Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ffs have seen an extraordinary range of applications, yet the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. In this work, we use cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods to determine a structure of a filamentous virus including the tips. We show that structure combined with mutagenesis can identify phage domains that are important in bacterial attack and for release of new progeny, allowing new models to be proposed for the phage lifecycle. |
| first_indexed | 2024-04-09T12:48:34Z |
| format | Article |
| id | doaj.art-98015dc4b2d946138e69693418cb0023 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T12:48:34Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-98015dc4b2d946138e69693418cb00232023-05-14T11:22:10ZengNature PortfolioNature Communications2041-17232023-05-0114111510.1038/s41467-023-37915-wCryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assemblyRebecca Conners0Rayén Ignacia León-Quezada1Mathew McLaren2Nicholas J. Bennett3Bertram Daum4Jasna Rakonjac5Vicki A. M. Gold6Living Systems Institute, University of ExeterSchool of Natural Sciences, Massey UniversityLiving Systems Institute, University of ExeterSchool of Natural Sciences, Massey UniversityLiving Systems Institute, University of ExeterSchool of Natural Sciences, Massey UniversityLiving Systems Institute, University of ExeterAbstract Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ffs have seen an extraordinary range of applications, yet the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. In this work, we use cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods to determine a structure of a filamentous virus including the tips. We show that structure combined with mutagenesis can identify phage domains that are important in bacterial attack and for release of new progeny, allowing new models to be proposed for the phage lifecycle.https://doi.org/10.1038/s41467-023-37915-w |
| spellingShingle | Rebecca Conners Rayén Ignacia León-Quezada Mathew McLaren Nicholas J. Bennett Bertram Daum Jasna Rakonjac Vicki A. M. Gold Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly Nature Communications |
| title | Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| title_full | Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| title_fullStr | Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| title_full_unstemmed | Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| title_short | Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| title_sort | cryo electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly |
| url | https://doi.org/10.1038/s41467-023-37915-w |
| work_keys_str_mv | AT rebeccaconners cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT rayenignacialeonquezada cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT mathewmclaren cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT nicholasjbennett cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT bertramdaum cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT jasnarakonjac cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly AT vickiamgold cryoelectronmicroscopyofthef1filamentousphagerevealsinsightsintoviralinfectionandassembly |