Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study
IntroductionPlatelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.ObjectivesTo determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (W...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.866547/full |
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| author | Laura Miguel-Pastor Katy Satué Deborah Chicharro Marta Torres-Torrillas Ayla del Romero Pau Peláez José M. Carrillo José M. Carrillo Belén Cuervo Joaquín J. Sopena Joaquín J. Sopena José J. Cerón Mónica Rubio Mónica Rubio |
| author_facet | Laura Miguel-Pastor Katy Satué Deborah Chicharro Marta Torres-Torrillas Ayla del Romero Pau Peláez José M. Carrillo José M. Carrillo Belén Cuervo Joaquín J. Sopena Joaquín J. Sopena José J. Cerón Mónica Rubio Mónica Rubio |
| author_sort | Laura Miguel-Pastor |
| collection | DOAJ |
| description | IntroductionPlatelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.ObjectivesTo determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (WBC) between WB samples, PRP and platelet poor plasma (PPP) samples; and to analyze the concentrations of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in the PRP and PPP of healthy adult cats using a standardized protocol with PRGF®-Endoret® characteristics.Material and MethodsWB was collected from 30 cats. PRP was obtained following three centrifugation protocols using PRGF®-Endoret® technology: 255, 260, and 265 g for 10 min each. The cellular components, RBC, WBC, PLT, and the concentrations of PDGF-BB and TGF-β1 in the PRP and PPP fractions were determined for each protocol.ResultsPLTs in the PRP fraction were statistically higher than WB, with no statistical differences between PPP and WB. In PRP fraction, PLT concentration was increased 1.4 times on average at 255 g; 1.3 times at 260 g and, 1.5 times at 265 g without statistical differences among them. The mean platelet volume (MPV) was significantly higher in WB compared to PRP and PPP fractions without significant differences between protocols. Compared to WB, the number of RBCs and WBCs was reduced by 99% and by more than 95% in PRP and PPP respectively, without significant differences between protocols. PDGF-BB concentrations were statistically higher in PRP than in PPP fractions, however, TGF-ß1 concentrations did not vary between fractions at 260 g. Comparing the three protocols within PRP and PPP fractions, no differences in PDGF-BB and TGF-ß1 concentrations were observed.Clinical RelevanceThe study shows scientific evidence regarding the obtention of PRP in cats using the PRGF®-Endoret® technology for the quantification of PDGF-BB and TGF-ß1. At 265 g for 10 min, PLT concentration was increased 1.5 times with unnoticeable erythrocytes and leukocytes in the samples. These results clearly show that the PRGF®-Endoret® methodology is suitable to obtain PRP in cats. Further studies are needed to determine the clinical efficacy of the obtained PGRF in the treatment of different pathologies in cats. |
| first_indexed | 2024-12-12T09:57:02Z |
| format | Article |
| id | doaj.art-9801c7a5959b455b8ee93d4d3668d00e |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-12T09:57:02Z |
| publishDate | 2022-04-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-9801c7a5959b455b8ee93d4d3668d00e2022-12-22T00:28:06ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-04-01910.3389/fvets.2022.866547866547Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective StudyLaura Miguel-Pastor0Katy Satué1Deborah Chicharro2Marta Torres-Torrillas3Ayla del Romero4Pau Peláez5José M. Carrillo6José M. Carrillo7Belén Cuervo8Joaquín J. Sopena9Joaquín J. Sopena10José J. Cerón11Mónica Rubio12Mónica Rubio13Bioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainGarcía Cugat Foundation CEU-UCH Chair of Medicine and Regenerative Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainGarcía Cugat Foundation CEU-UCH Chair of Medicine and Regenerative Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainInterdisciplinary Laboratory of Clinical Analysis, University of Murcia, Murcia, SpainBioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainGarcía Cugat Foundation CEU-UCH Chair of Medicine and Regenerative Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, SpainIntroductionPlatelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.ObjectivesTo determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (WBC) between WB samples, PRP and platelet poor plasma (PPP) samples; and to analyze the concentrations of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in the PRP and PPP of healthy adult cats using a standardized protocol with PRGF®-Endoret® characteristics.Material and MethodsWB was collected from 30 cats. PRP was obtained following three centrifugation protocols using PRGF®-Endoret® technology: 255, 260, and 265 g for 10 min each. The cellular components, RBC, WBC, PLT, and the concentrations of PDGF-BB and TGF-β1 in the PRP and PPP fractions were determined for each protocol.ResultsPLTs in the PRP fraction were statistically higher than WB, with no statistical differences between PPP and WB. In PRP fraction, PLT concentration was increased 1.4 times on average at 255 g; 1.3 times at 260 g and, 1.5 times at 265 g without statistical differences among them. The mean platelet volume (MPV) was significantly higher in WB compared to PRP and PPP fractions without significant differences between protocols. Compared to WB, the number of RBCs and WBCs was reduced by 99% and by more than 95% in PRP and PPP respectively, without significant differences between protocols. PDGF-BB concentrations were statistically higher in PRP than in PPP fractions, however, TGF-ß1 concentrations did not vary between fractions at 260 g. Comparing the three protocols within PRP and PPP fractions, no differences in PDGF-BB and TGF-ß1 concentrations were observed.Clinical RelevanceThe study shows scientific evidence regarding the obtention of PRP in cats using the PRGF®-Endoret® technology for the quantification of PDGF-BB and TGF-ß1. At 265 g for 10 min, PLT concentration was increased 1.5 times with unnoticeable erythrocytes and leukocytes in the samples. These results clearly show that the PRGF®-Endoret® methodology is suitable to obtain PRP in cats. Further studies are needed to determine the clinical efficacy of the obtained PGRF in the treatment of different pathologies in cats.https://www.frontiersin.org/articles/10.3389/fvets.2022.866547/fullcatplatelet countPlatelet-Rich Plasmaplatelet-derived growth factor BBtransforming growth factor β1PRGF® |
| spellingShingle | Laura Miguel-Pastor Katy Satué Deborah Chicharro Marta Torres-Torrillas Ayla del Romero Pau Peláez José M. Carrillo José M. Carrillo Belén Cuervo Joaquín J. Sopena Joaquín J. Sopena José J. Cerón Mónica Rubio Mónica Rubio Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study Frontiers in Veterinary Science cat platelet count Platelet-Rich Plasma platelet-derived growth factor BB transforming growth factor β1 PRGF® |
| title | Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study |
| title_full | Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study |
| title_fullStr | Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study |
| title_full_unstemmed | Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study |
| title_short | Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study |
| title_sort | evaluation of a standardized protocol for plasma rich in growth factors obtention in cats a prospective study |
| topic | cat platelet count Platelet-Rich Plasma platelet-derived growth factor BB transforming growth factor β1 PRGF® |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.866547/full |
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