L-Asparaginase-Based Biosensors
L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods c...
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MDPI AG
2021-08-01
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| Series: | Encyclopedia |
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| Online Access: | https://www.mdpi.com/2673-8392/1/3/65 |
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| author | João C. F. Nunes Raquel O. Cristóvão Valéria C. Santos-Ebinuma Joaquim L. Faria Cláudia G. Silva Márcia C. Neves Mara G. Freire Ana P. M. Tavares |
| author_facet | João C. F. Nunes Raquel O. Cristóvão Valéria C. Santos-Ebinuma Joaquim L. Faria Cláudia G. Silva Márcia C. Neves Mara G. Freire Ana P. M. Tavares |
| author_sort | João C. F. Nunes |
| collection | DOAJ |
| description | L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response. |
| first_indexed | 2024-03-10T07:43:43Z |
| format | Article |
| id | doaj.art-980237c3f78f4394a984178fe4f02ea9 |
| institution | Directory Open Access Journal |
| issn | 2673-8392 |
| language | English |
| last_indexed | 2024-03-10T07:43:43Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Encyclopedia |
| spelling | doaj.art-980237c3f78f4394a984178fe4f02ea92023-11-22T12:49:33ZengMDPI AGEncyclopedia2673-83922021-08-011384885810.3390/encyclopedia1030065L-Asparaginase-Based BiosensorsJoão C. F. Nunes0Raquel O. Cristóvão1Valéria C. Santos-Ebinuma2Joaquim L. Faria3Cláudia G. Silva4Márcia C. Neves5Mara G. Freire6Ana P. M. Tavares7CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLaboratory of Separation and Reaction Engineering-Laboratory of Catalysis and Materials (LSRE-LCM), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, PortugalDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista-UNESP, Araraquara 14800-903, BrazilLaboratory of Separation and Reaction Engineering-Laboratory of Catalysis and Materials (LSRE-LCM), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, PortugalLaboratory of Separation and Reaction Engineering-Laboratory of Catalysis and Materials (LSRE-LCM), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, PortugalCICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalCICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalCICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalL-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response.https://www.mdpi.com/2673-8392/1/3/65L-asparaginasebiosensorL-asparaginemonitoringammoniapharmaceutical |
| spellingShingle | João C. F. Nunes Raquel O. Cristóvão Valéria C. Santos-Ebinuma Joaquim L. Faria Cláudia G. Silva Márcia C. Neves Mara G. Freire Ana P. M. Tavares L-Asparaginase-Based Biosensors Encyclopedia L-asparaginase biosensor L-asparagine monitoring ammonia pharmaceutical |
| title | L-Asparaginase-Based Biosensors |
| title_full | L-Asparaginase-Based Biosensors |
| title_fullStr | L-Asparaginase-Based Biosensors |
| title_full_unstemmed | L-Asparaginase-Based Biosensors |
| title_short | L-Asparaginase-Based Biosensors |
| title_sort | l asparaginase based biosensors |
| topic | L-asparaginase biosensor L-asparagine monitoring ammonia pharmaceutical |
| url | https://www.mdpi.com/2673-8392/1/3/65 |
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