Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma
Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlori...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2022-02-01
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| Series: | Biomedical Photonics |
| Subjects: | |
| Online Access: | https://www.pdt-journal.com/jour/article/view/516 |
| _version_ | 1797883649015676928 |
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| author | A. Yu. Rynda V. E. Olyushin D. M. Rostovtsev Y. M. Zabrodskaya G. V. Papayan |
| author_facet | A. Yu. Rynda V. E. Olyushin D. M. Rostovtsev Y. M. Zabrodskaya G. V. Papayan |
| author_sort | A. Yu. Rynda |
| collection | DOAJ |
| description | Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlorin e6 was used for intraoperative fluorescence control at a dose of 1 mg/kg. During the operation, the fluorescence intensity of various areas of the putative tumor tissue was analyzed using the RSS Cam – Endo 1.4.313 software. Tissue samples with various degrees of fluorescence intensity were compared with the results of their histopathological analysis (WHO tumor diagnosis, Ki-67 index, P53, VEGF). Fluorescence was detected in more than half of the cases, but in most cases had a focal character and low fluorescence intensity. The fluorescence intensity directly correlated with the data of histopathological examination of tumor tissues (Ki-67 index (p=0.002), expression of P53 (p=0.0015) and VEGF (p=0.001)). The sensitivity of the method for LGG surgery was 72%, the specificity was 56,7%. Intraoperative fluorescence diagnostics with chlorin e6 can be used in LGG surgery, especially to visualize intratumoral areas with a higher degree of anaplasia. |
| first_indexed | 2024-04-10T03:55:10Z |
| format | Article |
| id | doaj.art-9806d730b9ef486983bf7e7640d69826 |
| institution | Directory Open Access Journal |
| issn | 2413-9432 |
| language | Russian |
| last_indexed | 2024-04-10T03:55:10Z |
| publishDate | 2022-02-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj.art-9806d730b9ef486983bf7e7640d698262023-03-13T07:12:31ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322022-02-01104354310.24931/2413-9432-2021-10-4-35-43241Fluorescent diagnostics with chlorin e6 in surgery of low-grade gliomaA. Yu. Rynda0V. E. Olyushin1D. M. Rostovtsev2Y. M. Zabrodskaya3G. V. Papayan4Российский нейрохирургический институт имени профессора А.Л. Поленова – филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»Российский нейрохирургический институт имени профессора А.Л. Поленова – филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»Российский нейрохирургический институт имени профессора А.Л. Поленова – филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»Российский нейрохирургический институт имени профессора А.Л. Поленова – филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»Российский нейрохирургический институт имени профессора А.Л. Поленова – филиал ФГБУ «Национальный медицинский исследовательский центр имени В.А. Алмазова»Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlorin e6 was used for intraoperative fluorescence control at a dose of 1 mg/kg. During the operation, the fluorescence intensity of various areas of the putative tumor tissue was analyzed using the RSS Cam – Endo 1.4.313 software. Tissue samples with various degrees of fluorescence intensity were compared with the results of their histopathological analysis (WHO tumor diagnosis, Ki-67 index, P53, VEGF). Fluorescence was detected in more than half of the cases, but in most cases had a focal character and low fluorescence intensity. The fluorescence intensity directly correlated with the data of histopathological examination of tumor tissues (Ki-67 index (p=0.002), expression of P53 (p=0.0015) and VEGF (p=0.001)). The sensitivity of the method for LGG surgery was 72%, the specificity was 56,7%. Intraoperative fluorescence diagnostics with chlorin e6 can be used in LGG surgery, especially to visualize intratumoral areas with a higher degree of anaplasia.https://www.pdt-journal.com/jour/article/view/516глиомы низкой степени злокачественностихлорин е6интраоперационная флуоресцентная диагностиканейроонкология |
| spellingShingle | A. Yu. Rynda V. E. Olyushin D. M. Rostovtsev Y. M. Zabrodskaya G. V. Papayan Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma Biomedical Photonics глиомы низкой степени злокачественности хлорин е6 интраоперационная флуоресцентная диагностика нейроонкология |
| title | Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma |
| title_full | Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma |
| title_fullStr | Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma |
| title_full_unstemmed | Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma |
| title_short | Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma |
| title_sort | fluorescent diagnostics with chlorin e6 in surgery of low grade glioma |
| topic | глиомы низкой степени злокачественности хлорин е6 интраоперационная флуоресцентная диагностика нейроонкология |
| url | https://www.pdt-journal.com/jour/article/view/516 |
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