Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
Objectives The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Methods Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to de...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2019-02-01
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| Series: | Clinical and Experimental Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.e-ceo.org/upload/pdf/ceo-2018-00486.pdf |
| _version_ | 1818309900447514624 |
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| author | Aysegul Batioglu-Karaaltin Ercüment Ovali Mehmet V Karaaltin Murat Yener Mehmet Yılmaz Fatma Eyüpoğlu Yetkin Zeki Yılmaz Erol Rüştü Bozkurt Necdet Demir Esma Konuk Ergun Süreyya Bozdağ Özgür Yiğit Harun Cansiz |
| author_facet | Aysegul Batioglu-Karaaltin Ercüment Ovali Mehmet V Karaaltin Murat Yener Mehmet Yılmaz Fatma Eyüpoğlu Yetkin Zeki Yılmaz Erol Rüştü Bozkurt Necdet Demir Esma Konuk Ergun Süreyya Bozdağ Özgür Yiğit Harun Cansiz |
| author_sort | Aysegul Batioglu-Karaaltin |
| collection | DOAJ |
| description | Objectives The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Methods Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. Results DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). Conclusion Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time. |
| first_indexed | 2024-12-13T07:37:31Z |
| format | Article |
| id | doaj.art-980ecc9637ef4b3cb5ec22ac719500f5 |
| institution | Directory Open Access Journal |
| issn | 1976-8710 2005-0720 |
| language | English |
| last_indexed | 2024-12-13T07:37:31Z |
| publishDate | 2019-02-01 |
| publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
| record_format | Article |
| series | Clinical and Experimental Otorhinolaryngology |
| spelling | doaj.art-980ecc9637ef4b3cb5ec22ac719500f52022-12-21T23:55:02ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202019-02-01121869410.21053/ceo.2018.00486599Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea TransplantationAysegul Batioglu-Karaaltin0Ercüment Ovali1Mehmet V Karaaltin2Murat Yener3Mehmet Yılmaz4Fatma Eyüpoğlu5Yetkin Zeki Yılmaz6Erol Rüştü Bozkurt7Necdet Demir8Esma Konuk9Ergun Süreyya Bozdağ10Özgür Yiğit11Harun Cansiz12 Department of Otolaryngology, Head and Neck Surgery, Istanbul University Cerrahpasa Medicine Faculty, Istanbul, Turkey Acibadem Labcell Laboratories, Istanbul, Turkey Department of Plastic and Reconstructive Surgery, Acibadem University School of Medicine, Istanbul, Turkey Department of Otolaryngology, Head and Neck Surgery, Istanbul University Cerrahpasa Medicine Faculty, Istanbul, Turkey Department of Otolaryngology, Head and Neck Surgery, Istanbul University Cerrahpasa Medicine Faculty, Istanbul, Turkey Acibadem Labcell Laboratories, Istanbul, Turkey Department of Otolaryngology, Head and Neck Surgery, Istanbul University Cerrahpasa Medicine Faculty, Istanbul, Turkey Department of Pathology, Istanbul Education and Research Hospital, Istanbul, Turkey Department of Histology and Embryology, Akdeniz University Medicine Faculty, Antalya, Turkey Department of Histology and Embryology, Akdeniz University Medicine Faculty, Antalya, Turkey Faculty of Mechanical Engineering, Department of Mechanical Engineering, Istanbul Technical University, Istanbul, Turkey Department of Otolaryngology, Head and Neck Surgery, Istanbul Education and Research Hospital, Istanbul, Turkey Department of Otolaryngology, Head and Neck Surgery, Istanbul University Cerrahpasa Medicine Faculty, Istanbul, TurkeyObjectives The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Methods Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. Results DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). Conclusion Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.http://www.e-ceo.org/upload/pdf/ceo-2018-00486.pdfTracheaScaffoldFreeze DryingTissue EngineeringDeoxycholic Acid |
| spellingShingle | Aysegul Batioglu-Karaaltin Ercüment Ovali Mehmet V Karaaltin Murat Yener Mehmet Yılmaz Fatma Eyüpoğlu Yetkin Zeki Yılmaz Erol Rüştü Bozkurt Necdet Demir Esma Konuk Ergun Süreyya Bozdağ Özgür Yiğit Harun Cansiz Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation Clinical and Experimental Otorhinolaryngology Trachea Scaffold Freeze Drying Tissue Engineering Deoxycholic Acid |
| title | Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation |
| title_full | Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation |
| title_fullStr | Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation |
| title_full_unstemmed | Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation |
| title_short | Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation |
| title_sort | decellularization of trachea with combined techniques for tissue engineered trachea transplantation |
| topic | Trachea Scaffold Freeze Drying Tissue Engineering Deoxycholic Acid |
| url | http://www.e-ceo.org/upload/pdf/ceo-2018-00486.pdf |
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