Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano
Abstract Background The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Zoological Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40851-019-0122-6 |
| _version_ | 1818504791998857216 |
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| author | Jakub Wudarski Kirill Ustyantsev Lisa Glazenburg Eugene Berezikov |
| author_facet | Jakub Wudarski Kirill Ustyantsev Lisa Glazenburg Eugene Berezikov |
| author_sort | Jakub Wudarski |
| collection | DOAJ |
| description | Abstract Background The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal’s biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. Results We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C–30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. Conclusions We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism. |
| first_indexed | 2024-12-10T21:42:02Z |
| format | Article |
| id | doaj.art-980f4024278d4d46b7b9d4871b170c0b |
| institution | Directory Open Access Journal |
| issn | 2056-306X |
| language | English |
| last_indexed | 2024-12-10T21:42:02Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Zoological Letters |
| spelling | doaj.art-980f4024278d4d46b7b9d4871b170c0b2022-12-22T01:32:29ZengBMCZoological Letters2056-306X2019-02-015111010.1186/s40851-019-0122-6Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignanoJakub Wudarski0Kirill Ustyantsev1Lisa Glazenburg2Eugene Berezikov3European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center GroningenInstitute of Cytology and GeneticsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center GroningenEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center GroningenAbstract Background The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal’s biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. Results We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C–30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. Conclusions We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism.http://link.springer.com/article/10.1186/s40851-019-0122-6TemperatureHeat shock responseFlatwormsMacrostomum lignanoFertilityRegeneration |
| spellingShingle | Jakub Wudarski Kirill Ustyantsev Lisa Glazenburg Eugene Berezikov Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano Zoological Letters Temperature Heat shock response Flatworms Macrostomum lignano Fertility Regeneration |
| title | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
| title_full | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
| title_fullStr | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
| title_full_unstemmed | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
| title_short | Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano |
| title_sort | influence of temperature on development reproduction and regeneration in the flatworm model organism macrostomum lignano |
| topic | Temperature Heat shock response Flatworms Macrostomum lignano Fertility Regeneration |
| url | http://link.springer.com/article/10.1186/s40851-019-0122-6 |
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