Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta
Considering that Actinidia kolomikta bears abundant white leaves on reproductive branches during blossoming, we hypothesized that the white leaves may maintain photosynthetic capacity by adjustments of leaf anatomy and physiological regulation. To test this hypothesis, leaf anatomy, gas exchange, ch...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.856732/full |
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| author | Miao Yu Li Chen Dong-huan Liu Dan Sun Guang-li Shi Yan Yin De-quan Wen Zhen-xing Wang Jun Ai |
| author_facet | Miao Yu Li Chen Dong-huan Liu Dan Sun Guang-li Shi Yan Yin De-quan Wen Zhen-xing Wang Jun Ai |
| author_sort | Miao Yu |
| collection | DOAJ |
| description | Considering that Actinidia kolomikta bears abundant white leaves on reproductive branches during blossoming, we hypothesized that the white leaves may maintain photosynthetic capacity by adjustments of leaf anatomy and physiological regulation. To test this hypothesis, leaf anatomy, gas exchange, chlorophyll a fluorescence, and the transcriptome were examined in white leaves of A. kolomikta during flowering. The palisade and spongy mesophyll in the white leaves were thicker than those in green ones. Chloroplast development in palisade parenchyma of white leaves was abnormal, whereas spongy parenchyma of white leaves contained functional chloroplasts. The highest photosynthetic rate of white leaves was ~82% of that of green leaves over the course of the day. In addition, the maximum quantum yield of PSII (Fv/Fm) of the palisade mesophyll in white leaves was significantly lower than those of green ones, whereas Fv/Fm and quantum yield for electron transport were significantly higher in the spongy mesophyll of white leaves. Photosynthetic capacity regulation of white leaf also was attributed to upregulation or downregulation of some key genes involving in photosynthesis. Particularly, upregulation of sucrose phosphate synthase (SPS), glyeraldehyde-3-phosphate dehydrogenase (GAPDH) and RuBisCO activase (RCA) in white leaf suggested that they might be involved in regulation of sugar synthesis and Rubisco activase in maintaining photosynthetic capacity of white leaf. Conclusions: white leaves contained a thicker mesophyll layer and higher photosynthetic activity in spongy parenchyma cells than those of palisade parenchyma cells. This may compensate for the lowered photosynthetic capacity of the palisade mesophyll. Consequently, white leaves maintain a relatively high photosynthetic capacity in the field. |
| first_indexed | 2024-12-12T04:40:58Z |
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| id | doaj.art-9811270be1ab4e6890b4a29702e93dba |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T04:40:58Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9811270be1ab4e6890b4a29702e93dba2022-12-22T00:37:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.856732856732Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomiktaMiao Yu0Li Chen1Dong-huan Liu2Dan Sun3Guang-li Shi4Yan Yin5De-quan Wen6Zhen-xing Wang7Jun Ai8Laboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaBeijing Botanical Garden, Beijing, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Plant Resources, State Key Laboratory of Systematic and Envolutionary Botany, State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaLaboratory of Wild Fruit Physiology, College of Horticulture, Jilin Agricultural University, Changchun, ChinaConsidering that Actinidia kolomikta bears abundant white leaves on reproductive branches during blossoming, we hypothesized that the white leaves may maintain photosynthetic capacity by adjustments of leaf anatomy and physiological regulation. To test this hypothesis, leaf anatomy, gas exchange, chlorophyll a fluorescence, and the transcriptome were examined in white leaves of A. kolomikta during flowering. The palisade and spongy mesophyll in the white leaves were thicker than those in green ones. Chloroplast development in palisade parenchyma of white leaves was abnormal, whereas spongy parenchyma of white leaves contained functional chloroplasts. The highest photosynthetic rate of white leaves was ~82% of that of green leaves over the course of the day. In addition, the maximum quantum yield of PSII (Fv/Fm) of the palisade mesophyll in white leaves was significantly lower than those of green ones, whereas Fv/Fm and quantum yield for electron transport were significantly higher in the spongy mesophyll of white leaves. Photosynthetic capacity regulation of white leaf also was attributed to upregulation or downregulation of some key genes involving in photosynthesis. Particularly, upregulation of sucrose phosphate synthase (SPS), glyeraldehyde-3-phosphate dehydrogenase (GAPDH) and RuBisCO activase (RCA) in white leaf suggested that they might be involved in regulation of sugar synthesis and Rubisco activase in maintaining photosynthetic capacity of white leaf. Conclusions: white leaves contained a thicker mesophyll layer and higher photosynthetic activity in spongy parenchyma cells than those of palisade parenchyma cells. This may compensate for the lowered photosynthetic capacity of the palisade mesophyll. Consequently, white leaves maintain a relatively high photosynthetic capacity in the field.https://www.frontiersin.org/articles/10.3389/fpls.2022.856732/fullwhite leafphotosynthetic capacityleaf structurechlorophyll fluorescencespongy mesophyll cells |
| spellingShingle | Miao Yu Li Chen Dong-huan Liu Dan Sun Guang-li Shi Yan Yin De-quan Wen Zhen-xing Wang Jun Ai Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta Frontiers in Plant Science white leaf photosynthetic capacity leaf structure chlorophyll fluorescence spongy mesophyll cells |
| title | Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta |
| title_full | Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta |
| title_fullStr | Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta |
| title_full_unstemmed | Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta |
| title_short | Enhancement of Photosynthetic Capacity in Spongy Mesophyll Cells in White Leaves of Actinidia kolomikta |
| title_sort | enhancement of photosynthetic capacity in spongy mesophyll cells in white leaves of actinidia kolomikta |
| topic | white leaf photosynthetic capacity leaf structure chlorophyll fluorescence spongy mesophyll cells |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.856732/full |
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