First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea
Stem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2020-03-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2020.26.1.29 |
| _version_ | 1818973019919351808 |
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| author | Young Mi Ko Jiyoung Choi Yeong Hee Lee Heung Tae Kim |
| author_facet | Young Mi Ko Jiyoung Choi Yeong Hee Lee Heung Tae Kim |
| author_sort | Young Mi Ko |
| collection | DOAJ |
| description | Stem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to develop. After September, microsclerotia began to form even on the surface of the stems that had exhibited water-soaking symptom. After mid-October of the harvest season, the epidermis of the stem was peeled off, resulting in the formation of a large number of microsclerotia in the cortex. The pathogens isolated from these symptoms were the best in mycelial growth at 32–35°C, and the formation of microsclerotia was the most at 20–28°C. The pathogen was identified as Macrophomina phaseolina through the morphological characteristics of the pathogen and the sequencing of the internal transcribed spacer region gene. In addition, when inoculated with a soybean stem using toothpicks cultured with the pathogen, the same symptoms as seen on the soybean field occurred. When the pathogen was re-isolated at the lesion site, the same pathogen was isolated and identified as Macrophomina phaseolina. Based on the results, the disease is reported as soybean charcoal rot. |
| first_indexed | 2024-12-20T15:17:31Z |
| format | Article |
| id | doaj.art-98123c8442204560943ba1d1da4fb7bb |
| institution | Directory Open Access Journal |
| issn | 1598-2262 |
| language | English |
| last_indexed | 2024-12-20T15:17:31Z |
| publishDate | 2020-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-98123c8442204560943ba1d1da4fb7bb2022-12-21T19:36:09ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622020-03-01261293710.5423/RPD.2020.26.1.2910.5423RPD.2020.26.1.29First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in KoreaYoung Mi Ko0Jiyoung Choi1Yeong Hee Lee2Heung Tae Kim3Hwaseong city Agricultural Technology Center, Hwaseong 18316, KoreaDepartment of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University, Cheongju 28644, KoreaDivision of Horticultural Environment, NHRI, Wanju 55365, KoreaDepartment of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University, Cheongju 28644, KoreaStem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to develop. After September, microsclerotia began to form even on the surface of the stems that had exhibited water-soaking symptom. After mid-October of the harvest season, the epidermis of the stem was peeled off, resulting in the formation of a large number of microsclerotia in the cortex. The pathogens isolated from these symptoms were the best in mycelial growth at 32–35°C, and the formation of microsclerotia was the most at 20–28°C. The pathogen was identified as Macrophomina phaseolina through the morphological characteristics of the pathogen and the sequencing of the internal transcribed spacer region gene. In addition, when inoculated with a soybean stem using toothpicks cultured with the pathogen, the same symptoms as seen on the soybean field occurred. When the pathogen was re-isolated at the lesion site, the same pathogen was isolated and identified as Macrophomina phaseolina. Based on the results, the disease is reported as soybean charcoal rot.http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2020.26.1.29charcoal rotmacrophomina phaseolinastem blight of soybean |
| spellingShingle | Young Mi Ko Jiyoung Choi Yeong Hee Lee Heung Tae Kim First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea Research in Plant Disease charcoal rot macrophomina phaseolina stem blight of soybean |
| title | First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea |
| title_full | First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea |
| title_fullStr | First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea |
| title_full_unstemmed | First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea |
| title_short | First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea |
| title_sort | first report of charcoal rot caused by macrophomina phaseolina on glycine max in korea |
| topic | charcoal rot macrophomina phaseolina stem blight of soybean |
| url | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2020.26.1.29 |
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