ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish

ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish

In zebrafish, RNA-guided endonucleases such as Cas9 have enabled straightforward gene knockout and the construction of reporter lines or conditional alleles via targeted knockin strategies. However, the performance of another commonly used CRISPR system, Cas12a, is significantly limited due to both...

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Bibliographic Details
Main Authors: Bingzhou Han, Yage Zhang, Yang Zhou, Biao Zhang, Christopher J. Krueger, Xuetong Bi, Zuoyan Zhu, Xiangjun Tong, Bo Zhang
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Biology
Subjects:
ErCas12a
genome editing
knockin
MMEJ
NHEJ
zebrafish
Online Access:https://www.mdpi.com/2079-7737/11/3/411

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