Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation
Background: This study evaluated the influence of traumatic occlusion in the dentin-pulp complex a molar teeth submitted to subluxation. Material and methods: Ninety Wistar rats were divided into groups Naïve (N), Subluxation (S) and Subluxation with traumatic occlusion (STO) and submitted to histol...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905223002365 |
| _version_ | 1797302553400049664 |
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| author | Marina Fuzette Amaral Cláudio Aparecido Casatti Afsar Raza Naqvi Caio Vinicius Lourenço Debortoli Daniela Atili Brandini |
| author_facet | Marina Fuzette Amaral Cláudio Aparecido Casatti Afsar Raza Naqvi Caio Vinicius Lourenço Debortoli Daniela Atili Brandini |
| author_sort | Marina Fuzette Amaral |
| collection | DOAJ |
| description | Background: This study evaluated the influence of traumatic occlusion in the dentin-pulp complex a molar teeth submitted to subluxation. Material and methods: Ninety Wistar rats were divided into groups Naïve (N), Subluxation (S) and Subluxation with traumatic occlusion (STO) and submitted to histological analysis after 7 and 21 days. A quantitative analysis was submitted to one-way ANOVA and Tukey's post-hoc test, and Chi-square and Bonferronís post-hoc test. Results: S and STO showed a significant increase in blood vessels area (p < 0.0005), amorphous fundamental substance (p < 0.0005) and reactionary dentin formation (p < 0.0005), as well as a decrease in the nuclear profile (p < 0.0005), odontoblast layer (p = 0.013 and p < 0.0005) by day 7 when compared with N. These changes normalized by day 21, except for the reactionary dentin (p < 0.0005) in both S and STO groups. Interestingly, the STO group exhibited significant changes in the increase of pulp calcification (p < 0.0005), presence of tubules with nuclei (p < 0.0005), and inflammatory infiltrate (p < 0.0005), as well reduction of nuclear profile (p < 0.0005), odontoblast layer (p < 0.0005) compared with N and S at day 21. Conclusions: STO impaired the defence response and decreased pulp regeneration capacity by increasing the inflammatory infiltrate and pulp calcification, and decreasing the nucleated cell number in the odontoblast layer and central pulp. |
| first_indexed | 2024-03-07T23:39:32Z |
| format | Article |
| id | doaj.art-9812f0f94b3046c389f8ad99096ede1f |
| institution | Directory Open Access Journal |
| issn | 1013-9052 |
| language | English |
| last_indexed | 2024-03-07T23:39:32Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj.art-9812f0f94b3046c389f8ad99096ede1f2024-02-20T04:18:36ZengElsevierSaudi Dental Journal1013-90522024-02-01362321327Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxationMarina Fuzette Amaral0Cláudio Aparecido Casatti1Afsar Raza Naqvi2Caio Vinicius Lourenço Debortoli3Daniela Atili Brandini4Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, BrazilDepartment of Physiology and Morphology, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, BrazilMucosal Immunology Lab, The University of Illinois at Chicago, College of Dentistry, Dept. of Periodontics, Chicago, IL, United StatesDepartment of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, BrazilDepartment of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil; Corresponding author at: Discipline of Integrated Clinic, Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Rua José Bonifácio, 1193, Araçatuba, São Paulo CEP: 16015-050, Brazil.Background: This study evaluated the influence of traumatic occlusion in the dentin-pulp complex a molar teeth submitted to subluxation. Material and methods: Ninety Wistar rats were divided into groups Naïve (N), Subluxation (S) and Subluxation with traumatic occlusion (STO) and submitted to histological analysis after 7 and 21 days. A quantitative analysis was submitted to one-way ANOVA and Tukey's post-hoc test, and Chi-square and Bonferronís post-hoc test. Results: S and STO showed a significant increase in blood vessels area (p < 0.0005), amorphous fundamental substance (p < 0.0005) and reactionary dentin formation (p < 0.0005), as well as a decrease in the nuclear profile (p < 0.0005), odontoblast layer (p = 0.013 and p < 0.0005) by day 7 when compared with N. These changes normalized by day 21, except for the reactionary dentin (p < 0.0005) in both S and STO groups. Interestingly, the STO group exhibited significant changes in the increase of pulp calcification (p < 0.0005), presence of tubules with nuclei (p < 0.0005), and inflammatory infiltrate (p < 0.0005), as well reduction of nuclear profile (p < 0.0005), odontoblast layer (p < 0.0005) compared with N and S at day 21. Conclusions: STO impaired the defence response and decreased pulp regeneration capacity by increasing the inflammatory infiltrate and pulp calcification, and decreasing the nucleated cell number in the odontoblast layer and central pulp.http://www.sciencedirect.com/science/article/pii/S1013905223002365Wounds and InjuriesTraumatic dental occlusionDental pulpRats |
| spellingShingle | Marina Fuzette Amaral Cláudio Aparecido Casatti Afsar Raza Naqvi Caio Vinicius Lourenço Debortoli Daniela Atili Brandini Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation Saudi Dental Journal Wounds and Injuries Traumatic dental occlusion Dental pulp Rats |
| title | Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation |
| title_full | Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation |
| title_fullStr | Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation |
| title_full_unstemmed | Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation |
| title_short | Histological changes in pulp-dentin complex in tooth subjected to traumatic occlusion and subluxation |
| title_sort | histological changes in pulp dentin complex in tooth subjected to traumatic occlusion and subluxation |
| topic | Wounds and Injuries Traumatic dental occlusion Dental pulp Rats |
| url | http://www.sciencedirect.com/science/article/pii/S1013905223002365 |
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