Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs
Genetic analysis is a conventional way of identifying and monitoring captive and wildlife species. Knowledge of statistical parameters reinforcing their usefulness and effectiveness as powerful tools for preserving diversity is crucial. Although several studies have reported the diversity of cetacea...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/14/1857 |
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| author | Rocío Gómez Rocío M. Neri-Bazán Araceli Posadas-Mondragon Pablo A. Vizcaíno-Dorado Jonathan J. Magaña José Leopoldo Aguilar-Faisal |
| author_facet | Rocío Gómez Rocío M. Neri-Bazán Araceli Posadas-Mondragon Pablo A. Vizcaíno-Dorado Jonathan J. Magaña José Leopoldo Aguilar-Faisal |
| author_sort | Rocío Gómez |
| collection | DOAJ |
| description | Genetic analysis is a conventional way of identifying and monitoring captive and wildlife species. Knowledge of statistical parameters reinforcing their usefulness and effectiveness as powerful tools for preserving diversity is crucial. Although several studies have reported the diversity of cetaceans such as <i>Tursiops truncatus</i> using microsatellites, its informative degree has been poorly reported. Furthermore, the genetic structure of this cetacean has not been fully studied. In the present study, we selected 15 microsatellites with which 210 dolphins were genetically characterized using capillary electrophoresis. The genetic assertiveness of this set of hypervariable markers identified one individual in the range of 6.927e<sup>13</sup> to 1.806e<sup>16</sup>, demonstrating its substantial capability in kinship relationships. The genetic structure of these 210 dolphins was also determined regarding the putative capture origin; a genetic stratification (<i>k</i> = 2) was found. An additional dolphin group of undetermined origin was also characterized to challenge the proficiency of our chosen markers. The set of markers proposed herein could be a helpful tool to guarantee the maintenance of the genetic diversity rates in conservation programs both in <i>Tursiops truncatus</i> and across other odontocetes, Mysticeti and several genera of endangered and vulnerable species. |
| first_indexed | 2024-03-09T10:24:26Z |
| format | Article |
| id | doaj.art-9815c6c5b4e14c9e810012774300f7ff |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T10:24:26Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9815c6c5b4e14c9e810012774300f7ff2023-12-01T21:48:43ZengMDPI AGAnimals2076-26152022-07-011214185710.3390/ani12141857Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRsRocío Gómez0Rocío M. Neri-Bazán1Araceli Posadas-Mondragon2Pablo A. Vizcaíno-Dorado3Jonathan J. Magaña4José Leopoldo Aguilar-Faisal5Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico City 07360, MexicoLaboratorio de Medicina de Conservación, Escuela Superior de Medicina-Instituto Politécnico Nacional (ESM-IPN), Mexico City 11340, MexicoLaboratorio de Medicina de Conservación, Escuela Superior de Medicina-Instituto Politécnico Nacional (ESM-IPN), Mexico City 11340, MexicoLaboratorio de Medicina Genómica, Departamento de Genética, Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra-Ibarra (INR-LGII), Mexico City 14389, MexicoLaboratorio de Medicina Genómica, Departamento de Genética, Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra-Ibarra (INR-LGII), Mexico City 14389, MexicoLaboratorio de Medicina de Conservación, Escuela Superior de Medicina-Instituto Politécnico Nacional (ESM-IPN), Mexico City 11340, MexicoGenetic analysis is a conventional way of identifying and monitoring captive and wildlife species. Knowledge of statistical parameters reinforcing their usefulness and effectiveness as powerful tools for preserving diversity is crucial. Although several studies have reported the diversity of cetaceans such as <i>Tursiops truncatus</i> using microsatellites, its informative degree has been poorly reported. Furthermore, the genetic structure of this cetacean has not been fully studied. In the present study, we selected 15 microsatellites with which 210 dolphins were genetically characterized using capillary electrophoresis. The genetic assertiveness of this set of hypervariable markers identified one individual in the range of 6.927e<sup>13</sup> to 1.806e<sup>16</sup>, demonstrating its substantial capability in kinship relationships. The genetic structure of these 210 dolphins was also determined regarding the putative capture origin; a genetic stratification (<i>k</i> = 2) was found. An additional dolphin group of undetermined origin was also characterized to challenge the proficiency of our chosen markers. The set of markers proposed herein could be a helpful tool to guarantee the maintenance of the genetic diversity rates in conservation programs both in <i>Tursiops truncatus</i> and across other odontocetes, Mysticeti and several genera of endangered and vulnerable species.https://www.mdpi.com/2076-2615/12/14/1857<i>T. truncatus</i>conservation programsmicrosatellitesgene-flowdolphinspopulation genetics |
| spellingShingle | Rocío Gómez Rocío M. Neri-Bazán Araceli Posadas-Mondragon Pablo A. Vizcaíno-Dorado Jonathan J. Magaña José Leopoldo Aguilar-Faisal Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs Animals <i>T. truncatus</i> conservation programs microsatellites gene-flow dolphins population genetics |
| title | Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs |
| title_full | Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs |
| title_fullStr | Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs |
| title_full_unstemmed | Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs |
| title_short | Molecular Assessments, Statistical Effectiveness Parameters and Genetic Structure of Captive Populations of <i>Tursiops truncatus</i> Using 15 STRs |
| title_sort | molecular assessments statistical effectiveness parameters and genetic structure of captive populations of i tursiops truncatus i using 15 strs |
| topic | <i>T. truncatus</i> conservation programs microsatellites gene-flow dolphins population genetics |
| url | https://www.mdpi.com/2076-2615/12/14/1857 |
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