Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells
Non-resorbable polymeric nanoparticles (NPs) are proposed as an adjunctive treatment for bone regenerative strategies. The present in vitro investigation aimed to evaluate the effect of the different prototypes of bioactive NPs loaded with zinc (Zn-NPs), doxycycline (Dox-NPs) or dexamethasone (Dex-N...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/9/1865 |
| _version_ | 1827657836570607616 |
|---|---|
| author | Manuel Toledano-Osorio Sergio López-García Raquel Osorio Manuel Toledano David García-Bernal Sonia Sánchez-Bautista Francisco Javier Rodríguez-Lozano |
| author_facet | Manuel Toledano-Osorio Sergio López-García Raquel Osorio Manuel Toledano David García-Bernal Sonia Sánchez-Bautista Francisco Javier Rodríguez-Lozano |
| author_sort | Manuel Toledano-Osorio |
| collection | DOAJ |
| description | Non-resorbable polymeric nanoparticles (NPs) are proposed as an adjunctive treatment for bone regenerative strategies. The present in vitro investigation aimed to evaluate the effect of the different prototypes of bioactive NPs loaded with zinc (Zn-NPs), doxycycline (Dox-NPs) or dexamethasone (Dex-NPs) on the viability, morphology, migration, adhesion, osteoblastic differentiation, and mineralization potential of human bone marrow stem cells (hBMMSCs). Cell viability, proliferation, and differentiation were assessed using a resaruzin-based assay, cell cycle analysis, cell migration evaluation, cell cytoskeleton staining analysis, Alizarin Red S staining, and expression of the osteogenic-related genes by a real-time quantitative polymerase chain reaction (RT-qPCR). One-Way ANOVA and Tukey’s test were employed. The resazurin assay showed adequate cell viability considering all concentrations and types of NPs at 24, 48, and 72 h of culture. The cell cycle analysis revealed a regular cell cycle profile at 0.1, 1, and 10 µg/mL, whereas 100 µg/mL produced an arrest of cells in the S phase. Cells cultured with 0.1 and 1 µg/mL NP concentrations showed a similar migration capacity to the untreated group. After 21 days, mineralization was increased by all the NPs prototypes. Dox-NPs and Dex-NPs produced a generalized up-regulation of the osteogenic-related genes. Dex-NPs and Dox-NPs exhibited excellent osteogenic potential and promoted hBMMSC differentiation. Future investigations, both in vitro and in vivo, are required to confirm the suitability of these NPs for their clinical application. |
| first_indexed | 2024-03-09T22:50:32Z |
| format | Article |
| id | doaj.art-9815cb9e54b14b81bdad3b62107eec55 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:50:32Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-9815cb9e54b14b81bdad3b62107eec552023-11-23T18:22:00ZengMDPI AGPharmaceutics1999-49232022-09-01149186510.3390/pharmaceutics14091865Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem CellsManuel Toledano-Osorio0Sergio López-García1Raquel Osorio2Manuel Toledano3David García-Bernal4Sonia Sánchez-Bautista5Francisco Javier Rodríguez-Lozano6Faculty of Dentistry, University of Granada Colegio Máximo de Cartuja s/n, 18071 Granada, SpainDepartament d’Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, 46010 Valencia, SpainFaculty of Dentistry, University of Granada Colegio Máximo de Cartuja s/n, 18071 Granada, SpainFaculty of Dentistry, University of Granada Colegio Máximo de Cartuja s/n, 18071 Granada, SpainHematopoietic Transplant and Cellular Therapy Unit, Faculty of Medicine and Odontology, IMIB-Arrixaca, University of Murcia, 30120 Murcia, SpainDepartment of Health Sciences, Catholic University San Antonio of Murcia, 30107 Murcia, SpainHematopoietic Transplant and Cellular Therapy Unit, Faculty of Medicine and Odontology, IMIB-Arrixaca, University of Murcia, 30120 Murcia, SpainNon-resorbable polymeric nanoparticles (NPs) are proposed as an adjunctive treatment for bone regenerative strategies. The present in vitro investigation aimed to evaluate the effect of the different prototypes of bioactive NPs loaded with zinc (Zn-NPs), doxycycline (Dox-NPs) or dexamethasone (Dex-NPs) on the viability, morphology, migration, adhesion, osteoblastic differentiation, and mineralization potential of human bone marrow stem cells (hBMMSCs). Cell viability, proliferation, and differentiation were assessed using a resaruzin-based assay, cell cycle analysis, cell migration evaluation, cell cytoskeleton staining analysis, Alizarin Red S staining, and expression of the osteogenic-related genes by a real-time quantitative polymerase chain reaction (RT-qPCR). One-Way ANOVA and Tukey’s test were employed. The resazurin assay showed adequate cell viability considering all concentrations and types of NPs at 24, 48, and 72 h of culture. The cell cycle analysis revealed a regular cell cycle profile at 0.1, 1, and 10 µg/mL, whereas 100 µg/mL produced an arrest of cells in the S phase. Cells cultured with 0.1 and 1 µg/mL NP concentrations showed a similar migration capacity to the untreated group. After 21 days, mineralization was increased by all the NPs prototypes. Dox-NPs and Dex-NPs produced a generalized up-regulation of the osteogenic-related genes. Dex-NPs and Dox-NPs exhibited excellent osteogenic potential and promoted hBMMSC differentiation. Future investigations, both in vitro and in vivo, are required to confirm the suitability of these NPs for their clinical application.https://www.mdpi.com/1999-4923/14/9/1865cell proliferationdexamethasonedoxycyclinenanoparticlesosteogenic differentiationstem cells |
| spellingShingle | Manuel Toledano-Osorio Sergio López-García Raquel Osorio Manuel Toledano David García-Bernal Sonia Sánchez-Bautista Francisco Javier Rodríguez-Lozano Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells Pharmaceutics cell proliferation dexamethasone doxycycline nanoparticles osteogenic differentiation stem cells |
| title | Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells |
| title_full | Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells |
| title_fullStr | Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells |
| title_full_unstemmed | Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells |
| title_short | Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells |
| title_sort | dexamethasone and doxycycline doped nanoparticles increase the differentiation potential of human bone marrow stem cells |
| topic | cell proliferation dexamethasone doxycycline nanoparticles osteogenic differentiation stem cells |
| url | https://www.mdpi.com/1999-4923/14/9/1865 |
| work_keys_str_mv | AT manueltoledanoosorio dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT sergiolopezgarcia dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT raquelosorio dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT manueltoledano dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT davidgarciabernal dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT soniasanchezbautista dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells AT franciscojavierrodriguezlozano dexamethasoneanddoxycyclinedopednanoparticlesincreasethedifferentiationpotentialofhumanbonemarrowstemcells |