Adipokine levels and their association with clinical disease severity in patients with dengue.
Adipokines have not been studied in acute dengue, despite their emerging role in inducing and regulating inflammation. Therefore, we sought to identify adipokine levels in patients with varying severities of acute dengue to understand their role in disease pathogenesis. We determined the levels of l...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-09-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0011613 |
| _version_ | 1797667855801516032 |
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| author | Heshan Kuruppu W P Rivindu H Wickramanayake Chandima Jeewandara Deneshan Peranantharajah H S Colambage Lahiru Perera Laksiri Gomes Ananda Wijewickrama Graham S Ogg Gathsaurie Neelika Malavige |
| author_facet | Heshan Kuruppu W P Rivindu H Wickramanayake Chandima Jeewandara Deneshan Peranantharajah H S Colambage Lahiru Perera Laksiri Gomes Ananda Wijewickrama Graham S Ogg Gathsaurie Neelika Malavige |
| author_sort | Heshan Kuruppu |
| collection | DOAJ |
| description | Adipokines have not been studied in acute dengue, despite their emerging role in inducing and regulating inflammation. Therefore, we sought to identify adipokine levels in patients with varying severities of acute dengue to understand their role in disease pathogenesis. We determined the levels of leptin, resistin, omentin, adiponectin, as well as IFNβ, and NS1 using quantitative ELISA in patients with dengue fever (DF = 49) and dengue haemorrhagic fever (DHF = 22) at admission (febrile phase) and at the time of discharge (recovery phase). The viral loads and serotypes of all samples were quantified using quantitative real-time RT-PCR. Resistin levels (p = 0.04) and omentin (p = 0.006) levels were significantly higher in patients who developed DHF. Omentin levels in the febrile phase also correlated with the AST (Spearman's r = 0.38, p = 0.001) and ALT levels (Spearman's r = 0.24, p = 0.04); as well as serum leptin levels with both AST (Spearman's r = 0.27, p = 0.02) and ALT (Spearman's r = 0.28, p = 0.02). Serum adiponectin levels in the febrile phase did not correlate with any of the other adipokines or with liver enzymes, but inversely correlated with CRP levels (Spearman's r = -0.31, p = 0.008). Although not significant (p = 0.14) serum IFNβ levels were lower in the febrile phase in those who progressed to develop DHF (median 0, IQR 0 to 39.4 pg/ml), compared to those who had DF (median 37.1, IQR 0 to 65.6 pg.ml). The data suggest that adipokines are likely to play a role in the pathogenesis of dengue, which should be further explored for the potential to be used as prognostic markers and as therapeutic targets. |
| first_indexed | 2024-03-11T20:20:36Z |
| format | Article |
| id | doaj.art-98162784aa424bd58110308f019fe5da |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-11T20:20:36Z |
| publishDate | 2023-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-98162784aa424bd58110308f019fe5da2023-10-03T05:31:50ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352023-09-01179e001161310.1371/journal.pntd.0011613Adipokine levels and their association with clinical disease severity in patients with dengue.Heshan KuruppuW P Rivindu H WickramanayakeChandima JeewandaraDeneshan PeranantharajahH S ColambageLahiru PereraLaksiri GomesAnanda WijewickramaGraham S OggGathsaurie Neelika MalavigeAdipokines have not been studied in acute dengue, despite their emerging role in inducing and regulating inflammation. Therefore, we sought to identify adipokine levels in patients with varying severities of acute dengue to understand their role in disease pathogenesis. We determined the levels of leptin, resistin, omentin, adiponectin, as well as IFNβ, and NS1 using quantitative ELISA in patients with dengue fever (DF = 49) and dengue haemorrhagic fever (DHF = 22) at admission (febrile phase) and at the time of discharge (recovery phase). The viral loads and serotypes of all samples were quantified using quantitative real-time RT-PCR. Resistin levels (p = 0.04) and omentin (p = 0.006) levels were significantly higher in patients who developed DHF. Omentin levels in the febrile phase also correlated with the AST (Spearman's r = 0.38, p = 0.001) and ALT levels (Spearman's r = 0.24, p = 0.04); as well as serum leptin levels with both AST (Spearman's r = 0.27, p = 0.02) and ALT (Spearman's r = 0.28, p = 0.02). Serum adiponectin levels in the febrile phase did not correlate with any of the other adipokines or with liver enzymes, but inversely correlated with CRP levels (Spearman's r = -0.31, p = 0.008). Although not significant (p = 0.14) serum IFNβ levels were lower in the febrile phase in those who progressed to develop DHF (median 0, IQR 0 to 39.4 pg/ml), compared to those who had DF (median 37.1, IQR 0 to 65.6 pg.ml). The data suggest that adipokines are likely to play a role in the pathogenesis of dengue, which should be further explored for the potential to be used as prognostic markers and as therapeutic targets.https://doi.org/10.1371/journal.pntd.0011613 |
| spellingShingle | Heshan Kuruppu W P Rivindu H Wickramanayake Chandima Jeewandara Deneshan Peranantharajah H S Colambage Lahiru Perera Laksiri Gomes Ananda Wijewickrama Graham S Ogg Gathsaurie Neelika Malavige Adipokine levels and their association with clinical disease severity in patients with dengue. PLoS Neglected Tropical Diseases |
| title | Adipokine levels and their association with clinical disease severity in patients with dengue. |
| title_full | Adipokine levels and their association with clinical disease severity in patients with dengue. |
| title_fullStr | Adipokine levels and their association with clinical disease severity in patients with dengue. |
| title_full_unstemmed | Adipokine levels and their association with clinical disease severity in patients with dengue. |
| title_short | Adipokine levels and their association with clinical disease severity in patients with dengue. |
| title_sort | adipokine levels and their association with clinical disease severity in patients with dengue |
| url | https://doi.org/10.1371/journal.pntd.0011613 |
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