Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up
Background: Crohn's disease (CD) has a chronic course, which its recurrence varies widely among different patients. In this study we prospectively analyzed blood samples of 19 CD patients. Alteration in transcription of inflammatory and anti-inflammatory cytokines was analyzed compared with ho...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2022-07-01
|
| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/26362 |
| _version_ | 1811344569950797824 |
|---|---|
| author | Fatemeh Ghasemi Vahid Basirat Maryam Izad Mohammad Javad Tavassolifar Mehdi Yaseri Nasser Ebrahimi Daryani Masoud Alebouyeh Mohammad Reza Pourmand |
| author_facet | Fatemeh Ghasemi Vahid Basirat Maryam Izad Mohammad Javad Tavassolifar Mehdi Yaseri Nasser Ebrahimi Daryani Masoud Alebouyeh Mohammad Reza Pourmand |
| author_sort | Fatemeh Ghasemi |
| collection | DOAJ |
| description |
Background: Crohn's disease (CD) has a chronic course, which its recurrence varies widely among different patients. In this study we prospectively analyzed blood samples of 19 CD patients. Alteration in transcription of inflammatory and anti-inflammatory cytokines was analyzed compared with household members after three month follow up.
Methods: CD patients were diagnosed based on clinical symptoms, endoscopic and histopathologic characteristics. Nineteen CD patients and their households were evaluated from Jun 2019 to Feb 2021 at Tehran university hospitals. CD activity score, biological, clinical and demographic data of the patients were recorded at two time point intervals. Bacteriological tests were done using aerobic and anaerobic blood cultures. To investigate transcriptional alterations, peripheral blood mononuclear cells (PBMCs) were isolated using Ficol centrifugation method and relative quantitative real-time PCR was done to determine the expression level of IFN-γ, TNF-α, IL10, and FOXP3 cytokines.
Results: Our results showed a correlation between fecal calprotectin level (709.8 ± 554.6), C-reactive protein concentration (18.1 ± 15.9), and erythrocyte sedimentation rate (30.4 ± 17.9) with disease activity (Flare/remission). IL10 and Foxp3 anti-inflammatory gene’s expression were significantly (P = 0.003 for IL10 and P = 0.008 Foxp3) higher during the flare and remission in patients with active disease respectively. Bacteriological examination showed infection with Streptococcus spp. and Clostridium spp. in two CD patients during flares, which was correlated with upregulation and down-regulation of IL10, TNF-α, IFN-γ and FOXP3 proteins, respectively.
Conclusion: Occurrence of bacteremia, and higher amount of CAP, CRP and ESR are correlated with higher level of transcription for inflammatory cytokines, which could effectively reflect the disease activity. Raise in FoxP3 transcription proposed change in Treg sub-population in PBMC or its activity during the CD remission phase.
|
| first_indexed | 2024-04-13T19:50:11Z |
| format | Article |
| id | doaj.art-981a855b1ca849ce8801a166dc581b58 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-04-13T19:50:11Z |
| publishDate | 2022-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-981a855b1ca849ce8801a166dc581b582022-12-22T02:32:35ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932022-07-0151710.18502/ijph.v51i7.10099Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following upFatemeh Ghasemi0Vahid Basirat1Maryam Izad2Mohammad Javad Tavassolifar3Mehdi Yaseri4Nasser Ebrahimi Daryani5Masoud Alebouyeh6Mohammad Reza Pourmand7Department of Pathobiology, Biotechnology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Gastroenterology, School of Medicine, Isfahan University of Medical Sciences and health services, Isfahan, Iran1. Department of Immunology , School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran1. Department of Immunology , School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Gastroenterology and Hepatology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranPediatric Infections Research Centre, Research Institute for Children's Health, Shahid Beheshti University of Medi-cal Sciences, Tehran, IranDepartment of Pathobiology, Biotechnology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background: Crohn's disease (CD) has a chronic course, which its recurrence varies widely among different patients. In this study we prospectively analyzed blood samples of 19 CD patients. Alteration in transcription of inflammatory and anti-inflammatory cytokines was analyzed compared with household members after three month follow up. Methods: CD patients were diagnosed based on clinical symptoms, endoscopic and histopathologic characteristics. Nineteen CD patients and their households were evaluated from Jun 2019 to Feb 2021 at Tehran university hospitals. CD activity score, biological, clinical and demographic data of the patients were recorded at two time point intervals. Bacteriological tests were done using aerobic and anaerobic blood cultures. To investigate transcriptional alterations, peripheral blood mononuclear cells (PBMCs) were isolated using Ficol centrifugation method and relative quantitative real-time PCR was done to determine the expression level of IFN-γ, TNF-α, IL10, and FOXP3 cytokines. Results: Our results showed a correlation between fecal calprotectin level (709.8 ± 554.6), C-reactive protein concentration (18.1 ± 15.9), and erythrocyte sedimentation rate (30.4 ± 17.9) with disease activity (Flare/remission). IL10 and Foxp3 anti-inflammatory gene’s expression were significantly (P = 0.003 for IL10 and P = 0.008 Foxp3) higher during the flare and remission in patients with active disease respectively. Bacteriological examination showed infection with Streptococcus spp. and Clostridium spp. in two CD patients during flares, which was correlated with upregulation and down-regulation of IL10, TNF-α, IFN-γ and FOXP3 proteins, respectively. Conclusion: Occurrence of bacteremia, and higher amount of CAP, CRP and ESR are correlated with higher level of transcription for inflammatory cytokines, which could effectively reflect the disease activity. Raise in FoxP3 transcription proposed change in Treg sub-population in PBMC or its activity during the CD remission phase. https://ijph.tums.ac.ir/index.php/ijph/article/view/26362Crohn’s diseaseInflammationCytokinesRegulatory T cellBlood culture |
| spellingShingle | Fatemeh Ghasemi Vahid Basirat Maryam Izad Mohammad Javad Tavassolifar Mehdi Yaseri Nasser Ebrahimi Daryani Masoud Alebouyeh Mohammad Reza Pourmand Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up Iranian Journal of Public Health Crohn’s disease Inflammation Cytokines Regulatory T cell Blood culture |
| title | Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up |
| title_full | Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up |
| title_fullStr | Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up |
| title_full_unstemmed | Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up |
| title_short | Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’s Disease Patients after Following up |
| title_sort | alteration of the inflammatory and anti inflammatory cytokine profiles of peripheral blood mononuclear cell in crohn s disease patients after following up |
| topic | Crohn’s disease Inflammation Cytokines Regulatory T cell Blood culture |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/26362 |
| work_keys_str_mv | AT fatemehghasemi alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT vahidbasirat alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT maryamizad alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT mohammadjavadtavassolifar alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT mehdiyaseri alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT nasserebrahimidaryani alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT masoudalebouyeh alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup AT mohammadrezapourmand alterationoftheinflammatoryandantiinflammatorycytokineprofilesofperipheralbloodmononuclearcellincrohnsdiseasepatientsafterfollowingup |