Targeted mutagenesis using CRISPR/Cas system in medaka

Targeted mutagenesis using CRISPR/Cas system in medaka

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful targeted mutagenesis using RGENs in medaka, Oryz...

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Bibliographic Details
Main Authors: Satoshi Ansai, Masato Kinoshita
Format: Article
Language:English
Published: The Company of Biologists 2014-04-01
Series:Biology Open
Subjects:
Medaka
CRISPR/Cas
Genome editing
Mutagenesis
Off-target alterations
Online Access:http://bio.biologists.org/content/3/5/362

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