Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy
Mapping the dielectric properties of cells with nanoscale spatial resolution can be an important tool in nanomedicine and nanotoxicity analysis, which can complement structural and mechanical nanoscale measurements. Recently we have shown that dielectric constant maps can be obtained on dried fixed...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/11/6/1402 |
| _version_ | 1827691503779053568 |
|---|---|
| author | Martí Checa Ruben Millan-Solsona Adrianna Glinkowska Mares Silvia Pujals Gabriel Gomila |
| author_facet | Martí Checa Ruben Millan-Solsona Adrianna Glinkowska Mares Silvia Pujals Gabriel Gomila |
| author_sort | Martí Checa |
| collection | DOAJ |
| description | Mapping the dielectric properties of cells with nanoscale spatial resolution can be an important tool in nanomedicine and nanotoxicity analysis, which can complement structural and mechanical nanoscale measurements. Recently we have shown that dielectric constant maps can be obtained on dried fixed cells in air environment by means of scanning dielectric force volume microscopy. Here, we demonstrate that such measurements can also be performed in the much more challenging case of fixed cells in liquid environment. Performing the measurements in liquid media contributes to preserve better the structure of the fixed cells, while also enabling accessing the local dielectric properties under fully hydrated conditions. The results shown in this work pave the way to address the nanoscale dielectric imaging of living cells, for which still further developments are required, as discussed here. |
| first_indexed | 2024-03-10T11:02:39Z |
| format | Article |
| id | doaj.art-981c23d8434d42c9b05b291a225d7065 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T11:02:39Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-981c23d8434d42c9b05b291a225d70652023-11-21T21:22:31ZengMDPI AGNanomaterials2079-49912021-05-01116140210.3390/nano11061402Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume MicroscopyMartí Checa0Ruben Millan-Solsona1Adrianna Glinkowska Mares2Silvia Pujals3Gabriel Gomila4Nanoscale Bioelectric Characterization, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, SpainNanoscale Bioelectric Characterization, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, SpainNanoscopy for Nanomedicine, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, SpainDepartament d’Enginyeria Electrònica i Biomèdica, Universitat de Barcelona, c/Martí i Franquès 1, 08028 Barcelona, SpainNanoscale Bioelectric Characterization, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, SpainMapping the dielectric properties of cells with nanoscale spatial resolution can be an important tool in nanomedicine and nanotoxicity analysis, which can complement structural and mechanical nanoscale measurements. Recently we have shown that dielectric constant maps can be obtained on dried fixed cells in air environment by means of scanning dielectric force volume microscopy. Here, we demonstrate that such measurements can also be performed in the much more challenging case of fixed cells in liquid environment. Performing the measurements in liquid media contributes to preserve better the structure of the fixed cells, while also enabling accessing the local dielectric properties under fully hydrated conditions. The results shown in this work pave the way to address the nanoscale dielectric imaging of living cells, for which still further developments are required, as discussed here.https://www.mdpi.com/2079-4991/11/6/1402scanning probe microscopy (SPM)electrostatic force microscopy (EFM)scanning dielectric microscopy (SDM)atomic force microscopy (AFM)dielectric propertiesfunctional microscopy |
| spellingShingle | Martí Checa Ruben Millan-Solsona Adrianna Glinkowska Mares Silvia Pujals Gabriel Gomila Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy Nanomaterials scanning probe microscopy (SPM) electrostatic force microscopy (EFM) scanning dielectric microscopy (SDM) atomic force microscopy (AFM) dielectric properties functional microscopy |
| title | Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy |
| title_full | Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy |
| title_fullStr | Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy |
| title_full_unstemmed | Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy |
| title_short | Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy |
| title_sort | dielectric imaging of fixed hela cells by in liquid scanning dielectric force volume microscopy |
| topic | scanning probe microscopy (SPM) electrostatic force microscopy (EFM) scanning dielectric microscopy (SDM) atomic force microscopy (AFM) dielectric properties functional microscopy |
| url | https://www.mdpi.com/2079-4991/11/6/1402 |
| work_keys_str_mv | AT marticheca dielectricimagingoffixedhelacellsbyinliquidscanningdielectricforcevolumemicroscopy AT rubenmillansolsona dielectricimagingoffixedhelacellsbyinliquidscanningdielectricforcevolumemicroscopy AT adriannaglinkowskamares dielectricimagingoffixedhelacellsbyinliquidscanningdielectricforcevolumemicroscopy AT silviapujals dielectricimagingoffixedhelacellsbyinliquidscanningdielectricforcevolumemicroscopy AT gabrielgomila dielectricimagingoffixedhelacellsbyinliquidscanningdielectricforcevolumemicroscopy |