Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues
Abstract Background The bacterial communities associated with mosquito eggs are an essential component of the mosquito microbiota, yet there are few studies characterizing and comparing the microbiota of mosquito eggs to other host tissues. Methods We sampled gravid female Culex pipiens L. and Culex...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04408-4 |
| _version_ | 1818972596119535616 |
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| author | Elijah O. Juma Chang-Hyun Kim Christopher Dunlap Brian F. Allan Chris M. Stone |
| author_facet | Elijah O. Juma Chang-Hyun Kim Christopher Dunlap Brian F. Allan Chris M. Stone |
| author_sort | Elijah O. Juma |
| collection | DOAJ |
| description | Abstract Background The bacterial communities associated with mosquito eggs are an essential component of the mosquito microbiota, yet there are few studies characterizing and comparing the microbiota of mosquito eggs to other host tissues. Methods We sampled gravid female Culex pipiens L. and Culex restuans Theobald from the field, allowed them to oviposit in the laboratory, and characterized the bacterial communities associated with their egg rafts and midguts for comparison through MiSeq sequencing of the 16S rRNA gene. Results Bacterial richness was higher in egg rafts than in midguts for both species, and higher in Cx pipiens than Cx. restuans. The midgut samples of Cx. pipiens and Cx. restuans were dominated by Providencia. Culex pipiens and Cx. restuans egg rafts samples were dominated by Ralstonia and Novosphingobium, respectively. NMDS ordination based on Bray-Curtis distance matrix revealed that egg-raft samples, or midgut tissues harbored similar bacterial communities regardless of the mosquito species. Within each mosquito species, there was a distinct clustering of bacterial communities between egg raft and midgut tissues. Conclusion These findings expand the list of described bacterial communities associated with Cx. pipiens and Cx. restuans and the additional characterization of the egg raft bacterial communities facilitates comparative analysis of mosquito host tissues, providing a basis for future studies seeking to understand any functional role of the bacterial communities in mosquito biology. |
| first_indexed | 2024-12-20T15:10:47Z |
| format | Article |
| id | doaj.art-981ea0b754964b5d923a7020675aa687 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-20T15:10:47Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-981ea0b754964b5d923a7020675aa6872022-12-21T19:36:19ZengBMCParasites & Vectors1756-33052020-10-0113111210.1186/s13071-020-04408-4Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissuesElijah O. Juma0Chang-Hyun Kim1Christopher Dunlap2Brian F. Allan3Chris M. Stone4Department of Entomology, University of Illinois at Urbana-ChampaignIllinois Natural History Survey, University of Illinois at Urbana-ChampaignCrop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of AgricultureDepartment of Entomology, University of Illinois at Urbana-ChampaignIllinois Natural History Survey, University of Illinois at Urbana-ChampaignAbstract Background The bacterial communities associated with mosquito eggs are an essential component of the mosquito microbiota, yet there are few studies characterizing and comparing the microbiota of mosquito eggs to other host tissues. Methods We sampled gravid female Culex pipiens L. and Culex restuans Theobald from the field, allowed them to oviposit in the laboratory, and characterized the bacterial communities associated with their egg rafts and midguts for comparison through MiSeq sequencing of the 16S rRNA gene. Results Bacterial richness was higher in egg rafts than in midguts for both species, and higher in Cx pipiens than Cx. restuans. The midgut samples of Cx. pipiens and Cx. restuans were dominated by Providencia. Culex pipiens and Cx. restuans egg rafts samples were dominated by Ralstonia and Novosphingobium, respectively. NMDS ordination based on Bray-Curtis distance matrix revealed that egg-raft samples, or midgut tissues harbored similar bacterial communities regardless of the mosquito species. Within each mosquito species, there was a distinct clustering of bacterial communities between egg raft and midgut tissues. Conclusion These findings expand the list of described bacterial communities associated with Cx. pipiens and Cx. restuans and the additional characterization of the egg raft bacterial communities facilitates comparative analysis of mosquito host tissues, providing a basis for future studies seeking to understand any functional role of the bacterial communities in mosquito biology.http://link.springer.com/article/10.1186/s13071-020-04408-4Mosquito egg raftMosquito midgutCulex pipiens l.Culex restuansBacterial communities |
| spellingShingle | Elijah O. Juma Chang-Hyun Kim Christopher Dunlap Brian F. Allan Chris M. Stone Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues Parasites & Vectors Mosquito egg raft Mosquito midgut Culex pipiens l. Culex restuans Bacterial communities |
| title | Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| title_full | Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| title_fullStr | Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| title_full_unstemmed | Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| title_short | Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| title_sort | culex pipiens and culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues |
| topic | Mosquito egg raft Mosquito midgut Culex pipiens l. Culex restuans Bacterial communities |
| url | http://link.springer.com/article/10.1186/s13071-020-04408-4 |
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