Multi-Enzymatic Cascade One-Pot Biosynthesis of 3′-Sialyllactose Using Engineered <i>Escherichia coli</i>

Multi-Enzymatic Cascade One-Pot Biosynthesis of 3′-Sialyllactose Using Engineered <i>Escherichia coli</i>

Among the human milk oligosaccharides (HMOs), one of the most abundant oligosaccharides and has great benefits for human health is 3′-sialyllactose (3′-SL). Given its important physiological functions and the lack of cost-effective production processes, we constructed an in vitro multi-enzymatic cof...

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Bibliographic Details
Main Authors: Zhongkui Li, Zhijian Ni, Xiangsong Chen, Gang Wang, Jinyong Wu, Jianming Yao
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Molecules
Subjects:
multi-enzymes
biosynthesis
3′-SL
cofactor regeneration
Online Access:https://www.mdpi.com/1420-3049/25/16/3567

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