The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons
Tonic GABAergic inhibition regulates neuronal excitability and has been implicated to be involved in both neurological and psychiatric diseases. We have previously shown that the endogenous peptide antisecretory factor (AF) decreases phasic GABAergic inhibition onto pyramidal CA1 neurons. In the pre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-01-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00013/full |
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| author | Joakim eStrandberg Catarina eLindquist Stefan eLange Fredrik eAsztely Eric eHanse |
| author_facet | Joakim eStrandberg Catarina eLindquist Stefan eLange Fredrik eAsztely Eric eHanse |
| author_sort | Joakim eStrandberg |
| collection | DOAJ |
| description | Tonic GABAergic inhibition regulates neuronal excitability and has been implicated to be involved in both neurological and psychiatric diseases. We have previously shown that the endogenous peptide antisecretory factor (AF) decreases phasic GABAergic inhibition onto pyramidal CA1 neurons. In the present study, using whole-cell patch-clamp recordings, we investigated the mechanisms behind this disinhibition of CA1 pyramidal neurons by AF. We found that application of AF to acute rat hippocampal slices resulted in a reduction of the frequency, but not of the amplitude, of spontaneous IPSCs (sIPSCs) in CA1 pyramidal neurons. Miniature IPSCs (mIPSCs), recorded in the presence of tetrodotoxin (TTX), were however not affected by AF, neither in CA1 pyramidal cells, nor in stratum radiatum interneurons. Instead, AF caused an increase of the tonic GABAA current in stratum radiatum interneurons, leaving the tonic GABAergic transmission in CA1 pyramidal cells unaffected. These results show that the endogenous peptide AF enhances tonic, but not phasic, GABAergic signalling in CA1 stratum radiatum interneurons, without affecting tonic GABAergic signalling in CA1 pyramidal neurons. We suggest that this increased tonic GABAergic signalling in GABAergic interneurons could be a mechanism for the AF-mediated disinhibition of pyramidal neurons. |
| first_indexed | 2024-12-10T15:53:30Z |
| format | Article |
| id | doaj.art-9822cb1001744544907121800fa4dd34 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T15:53:30Z |
| publishDate | 2014-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-9822cb1001744544907121800fa4dd342022-12-22T01:42:44ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-01-01810.3389/fncel.2014.0001370029The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneuronsJoakim eStrandberg0Catarina eLindquist1Stefan eLange2Fredrik eAsztely3Eric eHanse4Departement of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of GothenburgDepartement of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of GothenburgInstitute of Biomedicine, Sahlgrenska Academy, University of GothenburgDepartement of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of GothenburgDepartement of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of GothenburgTonic GABAergic inhibition regulates neuronal excitability and has been implicated to be involved in both neurological and psychiatric diseases. We have previously shown that the endogenous peptide antisecretory factor (AF) decreases phasic GABAergic inhibition onto pyramidal CA1 neurons. In the present study, using whole-cell patch-clamp recordings, we investigated the mechanisms behind this disinhibition of CA1 pyramidal neurons by AF. We found that application of AF to acute rat hippocampal slices resulted in a reduction of the frequency, but not of the amplitude, of spontaneous IPSCs (sIPSCs) in CA1 pyramidal neurons. Miniature IPSCs (mIPSCs), recorded in the presence of tetrodotoxin (TTX), were however not affected by AF, neither in CA1 pyramidal cells, nor in stratum radiatum interneurons. Instead, AF caused an increase of the tonic GABAA current in stratum radiatum interneurons, leaving the tonic GABAergic transmission in CA1 pyramidal cells unaffected. These results show that the endogenous peptide AF enhances tonic, but not phasic, GABAergic signalling in CA1 stratum radiatum interneurons, without affecting tonic GABAergic signalling in CA1 pyramidal neurons. We suggest that this increased tonic GABAergic signalling in GABAergic interneurons could be a mechanism for the AF-mediated disinhibition of pyramidal neurons.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00013/fullHippocampusGABACA1tonic inhibitionIPSC |
| spellingShingle | Joakim eStrandberg Catarina eLindquist Stefan eLange Fredrik eAsztely Eric eHanse The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons Frontiers in Cellular Neuroscience Hippocampus GABA CA1 tonic inhibition IPSC |
| title | The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons |
| title_full | The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons |
| title_fullStr | The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons |
| title_full_unstemmed | The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons |
| title_short | The endogenous peptide antisecretory factor promotes tonic GABAergic signalling in CA1 stratum radiatum interneurons |
| title_sort | endogenous peptide antisecretory factor promotes tonic gabaergic signalling in ca1 stratum radiatum interneurons |
| topic | Hippocampus GABA CA1 tonic inhibition IPSC |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00013/full |
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