The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus
Established autotetraploids often have a highly stable meiosis with high fertility compared with neo-autotetraploids. The autotetraploid Carassius auratus (4n = 200, RRRR) (4nRR), which stemmed from whole-genome duplication of Carassius auratus red var. (2n = 100, RR) (RCC), produces diploid gametes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1135006/full |
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| author | Xidan Xu Chongqing Wang Qingwen Xiao Xu Huang Yue Zhou Xiang Luo Yuxin Zhang Xiaowei Xu Qinbo Qin Shaojun Liu |
| author_facet | Xidan Xu Chongqing Wang Qingwen Xiao Xu Huang Yue Zhou Xiang Luo Yuxin Zhang Xiaowei Xu Qinbo Qin Shaojun Liu |
| author_sort | Xidan Xu |
| collection | DOAJ |
| description | Established autotetraploids often have a highly stable meiosis with high fertility compared with neo-autotetraploids. The autotetraploid Carassius auratus (4n = 200, RRRR) (4nRR), which stemmed from whole-genome duplication of Carassius auratus red var. (2n = 100, RR) (RCC), produces diploid gametes with an adopted diploid-like chromosome pairing in meiosis and maintains the formation of autotetraploid lineages. In this study, we focused on Dmc1, a meiosis-specific recombinase during the prophase of meiosis I, and elaborated on the genetic variation, alternative transcription, expression characterization, and epigenetic modification of Dmc1 in RCC and 4nRR. Two original Dmc1 from RCC were identified in 4nRR, and two duplicated Dmc1 differences in genetic composition were observed in 4nRR. Furthermore, we only noticed that one original and one duplicated Dmc1 were expressed in RCC and 4nRR, respectively. However, both possessed identical gene expression profiles, differential expression of sexual dimorphism, and hypomethylation levels. These results indicated that the specific expression of duplicated Dmc1 may be involve in the progression of meiosis of the diploid-like chromosome pairing in autotetraploid Carassius auratus. Herein, the findings significantly increase knowledge of meiosis of autopolyploid fish and provide meaningful insights into genetic breeding in polyploidy fish. |
| first_indexed | 2024-04-09T21:22:54Z |
| format | Article |
| id | doaj.art-9823409aff354ca3a353b49024bf9adf |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-09T21:22:54Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9823409aff354ca3a353b49024bf9adf2023-03-28T04:28:39ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-03-011410.3389/fgene.2023.11350061135006The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratusXidan XuChongqing WangQingwen XiaoXu HuangYue ZhouXiang LuoYuxin ZhangXiaowei XuQinbo QinShaojun LiuEstablished autotetraploids often have a highly stable meiosis with high fertility compared with neo-autotetraploids. The autotetraploid Carassius auratus (4n = 200, RRRR) (4nRR), which stemmed from whole-genome duplication of Carassius auratus red var. (2n = 100, RR) (RCC), produces diploid gametes with an adopted diploid-like chromosome pairing in meiosis and maintains the formation of autotetraploid lineages. In this study, we focused on Dmc1, a meiosis-specific recombinase during the prophase of meiosis I, and elaborated on the genetic variation, alternative transcription, expression characterization, and epigenetic modification of Dmc1 in RCC and 4nRR. Two original Dmc1 from RCC were identified in 4nRR, and two duplicated Dmc1 differences in genetic composition were observed in 4nRR. Furthermore, we only noticed that one original and one duplicated Dmc1 were expressed in RCC and 4nRR, respectively. However, both possessed identical gene expression profiles, differential expression of sexual dimorphism, and hypomethylation levels. These results indicated that the specific expression of duplicated Dmc1 may be involve in the progression of meiosis of the diploid-like chromosome pairing in autotetraploid Carassius auratus. Herein, the findings significantly increase knowledge of meiosis of autopolyploid fish and provide meaningful insights into genetic breeding in polyploidy fish.https://www.frontiersin.org/articles/10.3389/fgene.2023.1135006/fullautopolyploidizationmeiosisDMC1genetic/epigenetic diversityalternative transcription |
| spellingShingle | Xidan Xu Chongqing Wang Qingwen Xiao Xu Huang Yue Zhou Xiang Luo Yuxin Zhang Xiaowei Xu Qinbo Qin Shaojun Liu The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus Frontiers in Genetics autopolyploidization meiosis DMC1 genetic/epigenetic diversity alternative transcription |
| title | The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus |
| title_full | The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus |
| title_fullStr | The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus |
| title_full_unstemmed | The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus |
| title_short | The alternative transcription and expression characterization of Dmc1 in autotetraploid Carassius auratus |
| title_sort | alternative transcription and expression characterization of dmc1 in autotetraploid carassius auratus |
| topic | autopolyploidization meiosis DMC1 genetic/epigenetic diversity alternative transcription |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1135006/full |
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