Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate
Lactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in the packaging, automotive, food, and pharmaceutical industries. Previously, the yeast <i>Komagataella phaffii</i> was genetically modified for the production of lactate from glycerol. For this, the bovine...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/781 |
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| author | Nadielle Tamires Moreira Melo Gabriela Coimbra Pontes Dielle Pierotti Procópio Gabriel Caetano de Gois e Cunha Kevy Pontes Eliodório Hugo Costa Paes Thiago Olitta Basso Nádia Skorupa Parachin |
| author_facet | Nadielle Tamires Moreira Melo Gabriela Coimbra Pontes Dielle Pierotti Procópio Gabriel Caetano de Gois e Cunha Kevy Pontes Eliodório Hugo Costa Paes Thiago Olitta Basso Nádia Skorupa Parachin |
| author_sort | Nadielle Tamires Moreira Melo |
| collection | DOAJ |
| description | Lactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in the packaging, automotive, food, and pharmaceutical industries. Previously, the yeast <i>Komagataella phaffii</i> was genetically modified for the production of lactate from glycerol. For this, the bovine L-lactate dehydrogenase- (LDH)-encoding gene was inserted and the gene encoding the pyruvate decarboxylase (PDC) was disrupted, resulting in the GLp strain. This showed a yield of 67% L-lactic acid and 20% arabitol as a by-product in batches with oxygen limitation. Following up on these results, the present work endeavored to perform a detailed study of the metabolism of this yeast, as well as perturbing arabitol synthesis in an attempt to increase lactic acid titers. The GLp strain was cultivated in a glycerol-limited chemostat at different dilution rates, confirming that the production of both lactic acid and arabitol is dependent on the specific growth rate (and consequently on the concentration of the limiting carbon source) as well as on the oxygen level. Moreover, disruption of the gene encoding arabitol dehydrogenase (ArDH) was carried out, resulting in an increase of 20% in lactic acid and a 50% reduction in arabitol. This study clarifies the underlying metabolic reasons for arabitol formation in <i>K. phaffii</i> and points to ways for improving production of lactic acid using <i>K. phaffii</i> as a biocatalyst. |
| first_indexed | 2024-03-10T19:39:54Z |
| format | Article |
| id | doaj.art-98239e5e583c479aa6425ff5bd7bb658 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:39:54Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-98239e5e583c479aa6425ff5bd7bb6582023-11-20T01:23:04ZengMDPI AGMicroorganisms2076-26072020-05-018578110.3390/microorganisms8050781Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as SubstrateNadielle Tamires Moreira Melo0Gabriela Coimbra Pontes1Dielle Pierotti Procópio2Gabriel Caetano de Gois e Cunha3Kevy Pontes Eliodório4Hugo Costa Paes5Thiago Olitta Basso6Nádia Skorupa Parachin7Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF CEP 70790-160, BrazilGrupo de Engenharia de Biocatalisadores, Departamento de Biologia Celular, Instituto de Ciências Biológicas Bloco K, Universidade de Brasília (UnB), Campus Darcy Ribeiro, Brasilia, Federal District 70.790-900, BrazilDepartment of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo-SP 05508-010, BrazilDepartment of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo-SP 05508-010, BrazilDepartment of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo-SP 05508-010, BrazilClinical Medicine Division, University of Brasília Medical School, Darcy Ribeiro Campus, Asa Norte, Brasília, Federal District 70910-900, BrazilDepartment of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo-SP 05508-010, BrazilPós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF CEP 70790-160, BrazilLactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in the packaging, automotive, food, and pharmaceutical industries. Previously, the yeast <i>Komagataella phaffii</i> was genetically modified for the production of lactate from glycerol. For this, the bovine L-lactate dehydrogenase- (LDH)-encoding gene was inserted and the gene encoding the pyruvate decarboxylase (PDC) was disrupted, resulting in the GLp strain. This showed a yield of 67% L-lactic acid and 20% arabitol as a by-product in batches with oxygen limitation. Following up on these results, the present work endeavored to perform a detailed study of the metabolism of this yeast, as well as perturbing arabitol synthesis in an attempt to increase lactic acid titers. The GLp strain was cultivated in a glycerol-limited chemostat at different dilution rates, confirming that the production of both lactic acid and arabitol is dependent on the specific growth rate (and consequently on the concentration of the limiting carbon source) as well as on the oxygen level. Moreover, disruption of the gene encoding arabitol dehydrogenase (ArDH) was carried out, resulting in an increase of 20% in lactic acid and a 50% reduction in arabitol. This study clarifies the underlying metabolic reasons for arabitol formation in <i>K. phaffii</i> and points to ways for improving production of lactic acid using <i>K. phaffii</i> as a biocatalyst.https://www.mdpi.com/2076-2607/8/5/781<i>K. phaffii</i>lactic acidarabitolarabitol dehydrogenasecrude glycerolchemostat cultivation |
| spellingShingle | Nadielle Tamires Moreira Melo Gabriela Coimbra Pontes Dielle Pierotti Procópio Gabriel Caetano de Gois e Cunha Kevy Pontes Eliodório Hugo Costa Paes Thiago Olitta Basso Nádia Skorupa Parachin Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate Microorganisms <i>K. phaffii</i> lactic acid arabitol arabitol dehydrogenase crude glycerol chemostat cultivation |
| title | Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate |
| title_full | Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate |
| title_fullStr | Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate |
| title_full_unstemmed | Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate |
| title_short | Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate |
| title_sort | evaluation of product distribution in chemostat and batch fermentation in lactic acid producing i komagataella phaffii i strains utilizing glycerol as substrate |
| topic | <i>K. phaffii</i> lactic acid arabitol arabitol dehydrogenase crude glycerol chemostat cultivation |
| url | https://www.mdpi.com/2076-2607/8/5/781 |
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