Optimized CRISPR/Cas9 system for gene knockout in chicken DF1 cells

Optimized CRISPR/Cas9 system for gene knockout in chicken DF1 cells

ABSTRACT: The editing efficiency primarily hinders the utility of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in poultry. For a better understanding of the factors that influence the efficiency of gene knockout mediated by CRISPR/Cas9 in chicken DF1 cells, the singl...

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Bibliographic Details
Main Authors: Kexin Zou, Fang Wang, Zechun Zhang, Yang Zhou, Pengcheng Li, Dan Wang, Mengqi Zhu, Cunling Jia, Zehui Wei
Format: Article
Language:English
Published: Elsevier 2023-10-01
Series:Poultry Science
Subjects:
CRISPR
dual sgRNA
amplicon sequence
T7E1
DF1 cells
Online Access:http://www.sciencedirect.com/science/article/pii/S0032579123004893

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