Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor
Background: Rapid eye movement sleep deprivation (REMSD) and fluoxetine affect depression, yet the detailed molecular mechanisms were not clear.Methods: Rat depression chronic unpredictable stress was constructed, and the body weight of rats was measured. The efficacy of REMSD and fluoxetine on the...
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| Format: | Article |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Psychiatry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpsyt.2021.599399/full |
| _version_ | 1818649715068108800 |
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| author | Xuan Ju Shengdong Wang Pan Yan Chunyan Zhu Xiwen Hu Jiezheng Dong Zhonglin Tan |
| author_facet | Xuan Ju Shengdong Wang Pan Yan Chunyan Zhu Xiwen Hu Jiezheng Dong Zhonglin Tan |
| author_sort | Xuan Ju |
| collection | DOAJ |
| description | Background: Rapid eye movement sleep deprivation (REMSD) and fluoxetine affect depression, yet the detailed molecular mechanisms were not clear.Methods: Rat depression chronic unpredictable stress was constructed, and the body weight of rats was measured. The efficacy of REMSD and fluoxetine on the pleasure experience, exploration, and cognition of rats with depression was determined by the Sucrose preference test, the open field test, and Morris water task, respectively. The effects of REMSD and fluoxetine on depression-induced damage and apoptosis in rat hippocampi were detected using hematoxylin–eosin staining and terminal transferase-mediated biotin 2′-deoxyuridine, 5′-triphosphate nick end labeling. A1 adenosine receptor content was measured by immunohistochemistry. Relative expressions of the A1 adenosine receptor, proteins related to apoptosis (B Bcl-2-associated X protein; B-cell lymphoma 2), phosphoinositide 3-kinase, P38 mitogen-activated protein kinase, cFos, and adenosine deaminase RNA specific two were quantified by quantitative real-time polymerase chain reaction and Western blot as needed.Results: Depression decreased rat weight. REMSD combined with fluoxetine increased body weight, prompted rat behavior, alleviated depression-induced damage, attenuated apoptosis, and promoted A1 adenosine receptor level in rat hippocampi. Furthermore, the combined therapy upregulated expressions of A1 adenosine receptor, B-cell lymphoma 2, and phosphoinositide 3-kinase but downregulated those of B-cell lymphoma 2-associated X protein, P38 mitogen-activated protein kinase, cFos, and adenosine deaminase RNA specific 2 in the hippocampi of rats with depression.Conclusion:REMSD combined with fluoxetine protected rats against depression-induced damage and apoptosis in the hippocampus via the A1 adenosine receptor, providing a possible treatment strategy for depression. |
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| issn | 1664-0640 |
| language | English |
| last_indexed | 2024-12-17T01:38:43Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Psychiatry |
| spelling | doaj.art-982712fe44c94e90bb059a6c185d66fc2022-12-21T22:08:22ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402021-07-011210.3389/fpsyt.2021.599399599399Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine ReceptorXuan Ju0Shengdong Wang1Pan Yan2Chunyan Zhu3Xiwen Hu4Jiezheng Dong5Zhonglin Tan6Psychiatric Department, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaMolecular Biology Laboratory, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaMolecular Biology Laboratory, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaPsychiatric Department, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaPsychiatric Department, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaPsychiatric Department, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaPsychiatric Department, Hangzhou Seventh People's Hospital, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, ChinaBackground: Rapid eye movement sleep deprivation (REMSD) and fluoxetine affect depression, yet the detailed molecular mechanisms were not clear.Methods: Rat depression chronic unpredictable stress was constructed, and the body weight of rats was measured. The efficacy of REMSD and fluoxetine on the pleasure experience, exploration, and cognition of rats with depression was determined by the Sucrose preference test, the open field test, and Morris water task, respectively. The effects of REMSD and fluoxetine on depression-induced damage and apoptosis in rat hippocampi were detected using hematoxylin–eosin staining and terminal transferase-mediated biotin 2′-deoxyuridine, 5′-triphosphate nick end labeling. A1 adenosine receptor content was measured by immunohistochemistry. Relative expressions of the A1 adenosine receptor, proteins related to apoptosis (B Bcl-2-associated X protein; B-cell lymphoma 2), phosphoinositide 3-kinase, P38 mitogen-activated protein kinase, cFos, and adenosine deaminase RNA specific two were quantified by quantitative real-time polymerase chain reaction and Western blot as needed.Results: Depression decreased rat weight. REMSD combined with fluoxetine increased body weight, prompted rat behavior, alleviated depression-induced damage, attenuated apoptosis, and promoted A1 adenosine receptor level in rat hippocampi. Furthermore, the combined therapy upregulated expressions of A1 adenosine receptor, B-cell lymphoma 2, and phosphoinositide 3-kinase but downregulated those of B-cell lymphoma 2-associated X protein, P38 mitogen-activated protein kinase, cFos, and adenosine deaminase RNA specific 2 in the hippocampi of rats with depression.Conclusion:REMSD combined with fluoxetine protected rats against depression-induced damage and apoptosis in the hippocampus via the A1 adenosine receptor, providing a possible treatment strategy for depression.https://www.frontiersin.org/articles/10.3389/fpsyt.2021.599399/fulldepressionsleep deprivationfluoxetinehippocampusA1 adenosine receptor |
| spellingShingle | Xuan Ju Shengdong Wang Pan Yan Chunyan Zhu Xiwen Hu Jiezheng Dong Zhonglin Tan Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor Frontiers in Psychiatry depression sleep deprivation fluoxetine hippocampus A1 adenosine receptor |
| title | Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor |
| title_full | Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor |
| title_fullStr | Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor |
| title_full_unstemmed | Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor |
| title_short | Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor |
| title_sort | rapid eye movement sleep deprivation combined with fluoxetine protects against depression induced damage and apoptosis in rat hippocampi via a1 adenosine receptor |
| topic | depression sleep deprivation fluoxetine hippocampus A1 adenosine receptor |
| url | https://www.frontiersin.org/articles/10.3389/fpsyt.2021.599399/full |
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