Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach
The presence of antimicrobial-resistance genes (ARGs) in mobile genetic elements (MGEs) facilitates the rapid development and dissemination of multidrug-resistant bacteria, which represents a serious problem for human health. This is a One Health study which aims to investigate the co-occurrence of...
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MDPI AG
2022-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/8/1011 |
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| author | Hisham N. Altayb Hana S. Elbadawi Faisal A. Alzahrani Othman Baothman Imran Kazmi Muhammad Shahid Nadeem Salman Hosawi Kamel Chaieb |
| author_facet | Hisham N. Altayb Hana S. Elbadawi Faisal A. Alzahrani Othman Baothman Imran Kazmi Muhammad Shahid Nadeem Salman Hosawi Kamel Chaieb |
| author_sort | Hisham N. Altayb |
| collection | DOAJ |
| description | The presence of antimicrobial-resistance genes (ARGs) in mobile genetic elements (MGEs) facilitates the rapid development and dissemination of multidrug-resistant bacteria, which represents a serious problem for human health. This is a One Health study which aims to investigate the co-occurrence of antimicrobial resistance determinants among clinical and environmental isolates of <i>K. pneumoniae</i> and <i>E. coli</i>. Various bioinformatics tools were used to elucidate the bacterial strains’ ID, resistome, virulome, MGEs, and phylogeny for 42 isolates obtained from hospitalized patients (<i>n</i> = 20) and environmental sites (including fresh vegetables, fruits, and drinking water) (<i>n</i> = 22). The multilocus sequence typing (MLST) showed that <i>K. pneumoniae</i> belonged to ten sequence types (STs) while the <i>E. coli</i> belonged to seventeen STs. Multidrug-resistant isolates harbored β-lactam, aminoglycoside resistance determinants, and MGE were detected circulating in the environment (drinking water, fresh vegetables, and fruits) and in patients hospitalized with postoperative infections, neonatal sepsis, and urinary tract infection. Four <i>K. pneumoniae</i> environmental isolates (7E, 16EE, 1KE, and 19KE) were multidrug-resistant and were positive for different beta-lactam and aminoglycoside resistance determinants. <i>bla<sub>CTX-M-15</sub></i> in brackets of ISEc 9 and Tn 3 transposases was detected in isolates circulating in the pediatrics unit of Soba hospital and the environment. This study documented the presence of bacterial isolates harboring a similar pattern of antimicrobial resistance determinants circulating in hospitals and environments. A rapid response is needed from stakeholders to initiate a program for infection prevention and control measures to detect such clones disseminated in the communities and hospitals. |
| first_indexed | 2024-03-09T12:48:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T12:48:09Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-98288ba55deb4facbf8222bb83f6d8ca2023-11-30T22:10:32ZengMDPI AGPharmaceuticals1424-82472022-08-01158101110.3390/ph15081011Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic ApproachHisham N. Altayb0Hana S. Elbadawi1Faisal A. Alzahrani2Othman Baothman3Imran Kazmi4Muhammad Shahid Nadeem5Salman Hosawi6Kamel Chaieb7Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaMicrobiology and Parasitology Department, Soba University Hospital, University of Khartoum, Khartoum 11115, SudanDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaThe presence of antimicrobial-resistance genes (ARGs) in mobile genetic elements (MGEs) facilitates the rapid development and dissemination of multidrug-resistant bacteria, which represents a serious problem for human health. This is a One Health study which aims to investigate the co-occurrence of antimicrobial resistance determinants among clinical and environmental isolates of <i>K. pneumoniae</i> and <i>E. coli</i>. Various bioinformatics tools were used to elucidate the bacterial strains’ ID, resistome, virulome, MGEs, and phylogeny for 42 isolates obtained from hospitalized patients (<i>n</i> = 20) and environmental sites (including fresh vegetables, fruits, and drinking water) (<i>n</i> = 22). The multilocus sequence typing (MLST) showed that <i>K. pneumoniae</i> belonged to ten sequence types (STs) while the <i>E. coli</i> belonged to seventeen STs. Multidrug-resistant isolates harbored β-lactam, aminoglycoside resistance determinants, and MGE were detected circulating in the environment (drinking water, fresh vegetables, and fruits) and in patients hospitalized with postoperative infections, neonatal sepsis, and urinary tract infection. Four <i>K. pneumoniae</i> environmental isolates (7E, 16EE, 1KE, and 19KE) were multidrug-resistant and were positive for different beta-lactam and aminoglycoside resistance determinants. <i>bla<sub>CTX-M-15</sub></i> in brackets of ISEc 9 and Tn 3 transposases was detected in isolates circulating in the pediatrics unit of Soba hospital and the environment. This study documented the presence of bacterial isolates harboring a similar pattern of antimicrobial resistance determinants circulating in hospitals and environments. A rapid response is needed from stakeholders to initiate a program for infection prevention and control measures to detect such clones disseminated in the communities and hospitals.https://www.mdpi.com/1424-8247/15/8/1011mobile genetic elementsAMRESBLswhole genome sequencingMDR |
| spellingShingle | Hisham N. Altayb Hana S. Elbadawi Faisal A. Alzahrani Othman Baothman Imran Kazmi Muhammad Shahid Nadeem Salman Hosawi Kamel Chaieb Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach Pharmaceuticals mobile genetic elements AMR ESBLs whole genome sequencing MDR |
| title | Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach |
| title_full | Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach |
| title_fullStr | Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach |
| title_full_unstemmed | Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach |
| title_short | Co-Occurrence of β-Lactam and Aminoglycoside Resistance Determinants among Clinical and Environmental Isolates of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>: A Genomic Approach |
| title_sort | co occurrence of β lactam and aminoglycoside resistance determinants among clinical and environmental isolates of i klebsiella pneumoniae i and i escherichia coli i a genomic approach |
| topic | mobile genetic elements AMR ESBLs whole genome sequencing MDR |
| url | https://www.mdpi.com/1424-8247/15/8/1011 |
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