Differentiation of adult human mesenchymal stem cells into dopaminergic neurons
The striatal dopamine (DA) deficiency is known as the main cause of the clinical picture of Parkinson’s disease (PD). The disease is a progressive degeneration of dopaminergic neurons in the striatum. The treatment of PD is based on compensation for the brain’s supply of DA lost by drug therapy, dee...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=209;epage=215;aulast=Khademizadeh |
| _version_ | 1819126917326962688 |
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| author | Marjan Khademizadeh Manoochehr Messripour Nazem Ghasemi Fariborz Momen beik Ahmad Movahedian Attar |
| author_facet | Marjan Khademizadeh Manoochehr Messripour Nazem Ghasemi Fariborz Momen beik Ahmad Movahedian Attar |
| author_sort | Marjan Khademizadeh |
| collection | DOAJ |
| description | The striatal dopamine (DA) deficiency is known as the main cause of the clinical picture of Parkinson’s disease (PD). The disease is a progressive degeneration of dopaminergic neurons in the striatum. The treatment of PD is based on compensation for the brain’s supply of DA lost by drug therapy, deep brain stimulation, surgery, gene and cell therapies. Clinical studies have focused on the utility of stem cell-based therapies in PD. Embryonic and mesenchymal stem cells (MSCs) are widely used. Recently, human adipose derived stem cells (hADSCs) have been considered as a suitable source of tissue for this purpose. In this project, hADSCs differentiated into dopaminergic neurons and the specificity of the cell preparations was examined. Human adipose tissues were collected from healthy volunteers undergoing liposuction and hADSCs were isolated by collagenase-based enzymatic method. Flow cytometry was performed using the surface cluster of differentiation (CD) markers to confirm the cell typical properties. Then hADSCs were differentiated to dopaminergic neurons in neurobasal medium in the presence of differentiation factors and confirmed by immunocytochemistry via neuronal and dopaminergic markers. The isolated hADSCs were cultured and identified by the expression of MSCs surface markers including CD90, and CD44. These cells did not express hematopoietic surface markers such as CD45 and CD14. Differentiated cells express neuronal marker NeuN and dopaminergic marker tyrosine hydroxylase (TH). It is concluded that hADSCs can be easily taken from the patient’s own body and differentiated into dopaminergic cells having a lower risk of transplant rejection. |
| first_indexed | 2024-12-22T08:03:39Z |
| format | Article |
| id | doaj.art-982b387f315e42f6a5175be8d180eaf4 |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-22T08:03:39Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-982b387f315e42f6a5175be8d180eaf42022-12-21T18:33:12ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142019-01-0114320921510.4103/1735-5362.258487Differentiation of adult human mesenchymal stem cells into dopaminergic neuronsMarjan KhademizadehManoochehr MessripourNazem GhasemiFariborz Momen beikAhmad Movahedian AttarThe striatal dopamine (DA) deficiency is known as the main cause of the clinical picture of Parkinson’s disease (PD). The disease is a progressive degeneration of dopaminergic neurons in the striatum. The treatment of PD is based on compensation for the brain’s supply of DA lost by drug therapy, deep brain stimulation, surgery, gene and cell therapies. Clinical studies have focused on the utility of stem cell-based therapies in PD. Embryonic and mesenchymal stem cells (MSCs) are widely used. Recently, human adipose derived stem cells (hADSCs) have been considered as a suitable source of tissue for this purpose. In this project, hADSCs differentiated into dopaminergic neurons and the specificity of the cell preparations was examined. Human adipose tissues were collected from healthy volunteers undergoing liposuction and hADSCs were isolated by collagenase-based enzymatic method. Flow cytometry was performed using the surface cluster of differentiation (CD) markers to confirm the cell typical properties. Then hADSCs were differentiated to dopaminergic neurons in neurobasal medium in the presence of differentiation factors and confirmed by immunocytochemistry via neuronal and dopaminergic markers. The isolated hADSCs were cultured and identified by the expression of MSCs surface markers including CD90, and CD44. These cells did not express hematopoietic surface markers such as CD45 and CD14. Differentiated cells express neuronal marker NeuN and dopaminergic marker tyrosine hydroxylase (TH). It is concluded that hADSCs can be easily taken from the patient’s own body and differentiated into dopaminergic cells having a lower risk of transplant rejection.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=209;epage=215;aulast=Khademizadehadipose derived stem cells; differentiation; dopaminergic neurons. |
| spellingShingle | Marjan Khademizadeh Manoochehr Messripour Nazem Ghasemi Fariborz Momen beik Ahmad Movahedian Attar Differentiation of adult human mesenchymal stem cells into dopaminergic neurons Research in Pharmaceutical Sciences adipose derived stem cells; differentiation; dopaminergic neurons. |
| title | Differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| title_full | Differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| title_fullStr | Differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| title_full_unstemmed | Differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| title_short | Differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| title_sort | differentiation of adult human mesenchymal stem cells into dopaminergic neurons |
| topic | adipose derived stem cells; differentiation; dopaminergic neurons. |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=209;epage=215;aulast=Khademizadeh |
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