The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>)
The water buffalo (<i>Bubalus bubalis</i>) is known for its unique utilization of low-quality fibrous feeds and outstanding digestion performance, highlighting its role as an animal model in studying fiber fractions degradation. Among roughage, lignin attracted wide attention in ruminant...
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MDPI AG
2021-10-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/11/10/1015 |
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| author | Huimin Zhong Jiayan Zhou Mohamed Abdelrahman Hao Xu Zian Wu Luncheng Cui Zhenhua Ma Liguo Yang Xiang Li |
| author_facet | Huimin Zhong Jiayan Zhou Mohamed Abdelrahman Hao Xu Zian Wu Luncheng Cui Zhenhua Ma Liguo Yang Xiang Li |
| author_sort | Huimin Zhong |
| collection | DOAJ |
| description | The water buffalo (<i>Bubalus bubalis</i>) is known for its unique utilization of low-quality fibrous feeds and outstanding digestion performance, highlighting its role as an animal model in studying fiber fractions degradation. Among roughage, lignin attracted wide attention in ruminant nutrition studies, which affects animal digestibility. Therefore, the present study aims to investigate the functional relation between three lignin monomeric compositions of coniferyl alcohol (G), <i>ρ</i>-coumaryl alcohol (H) and sinapyl alcohol (S) and ruminal fiber degradation in water buffalo. Hence, three female water buffaloes (Nili-Ravi × Mediterranean, five years old, 480 ± 20 kg) were assigned for an in vivo study by utilizing the nylon-bag method, examining eight kinds of roughage. All the experimental roughage types were analyzed for the effective degradability (ED) of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL) and hemicellulose (HC) fractions. Then, prediction models for the roughage fiber degradation were established based on the characteristics of lignin monomer content. The results showed that S, S/G and S/(G+S+H) were positively correlated with the ED of NDF, ADF, CEL and HC; H/S was negatively correlated. For the effective degradability of ADL (ADLD), S and S/(G+S+H) were positively correlated with it; H, H/G, H/S and H/(G+S+H) were negatively correlated. The model with the highest fitting degree was ADLD = 0.161 − 1.918 × H + 3.152 × S (R<sup>2</sup> = 0.758, <i>p</i> < 0.01). These results indicated that the lignin monomer composition is closely related to the utilization rate of roughage fiber. S-type lignin monomer plays a vital role in the fiber degradation of roughage. The experiment found the effect of lignin monomer composition on the degradation of fiber fractions using buffalo as the experimental animal and constructed prediction models, providing a scientific basis for building a new technological method using lignin composition to evaluate buffalo roughage. Furthermore, the capacity of ADL degradation of buffalo was proved in this experiment. In order to further explore the ability of lignin degradation by the buffalo, the DNA of rumen microorganisms was extracted for sequencing. The top three composition of rumen microorganisms at the genus level were Prevotella_1, 226, Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG-011. Six strains with lignin degradation ability were screened from buffalo rumen contents. This experiment also revealed that the buffalos possess rumen microorganisms with lignin degradation potential. |
| first_indexed | 2024-03-09T04:40:14Z |
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| language | English |
| last_indexed | 2024-03-09T04:40:14Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-982b8f355ead4101a2edc9c8eee270e32023-12-03T13:22:37ZengMDPI AGAgriculture2077-04722021-10-011110101510.3390/agriculture11101015The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>)Huimin Zhong0Jiayan Zhou1Mohamed Abdelrahman2Hao Xu3Zian Wu4Luncheng Cui5Zhenhua Ma6Liguo Yang7Xiang Li8National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaThe water buffalo (<i>Bubalus bubalis</i>) is known for its unique utilization of low-quality fibrous feeds and outstanding digestion performance, highlighting its role as an animal model in studying fiber fractions degradation. Among roughage, lignin attracted wide attention in ruminant nutrition studies, which affects animal digestibility. Therefore, the present study aims to investigate the functional relation between three lignin monomeric compositions of coniferyl alcohol (G), <i>ρ</i>-coumaryl alcohol (H) and sinapyl alcohol (S) and ruminal fiber degradation in water buffalo. Hence, three female water buffaloes (Nili-Ravi × Mediterranean, five years old, 480 ± 20 kg) were assigned for an in vivo study by utilizing the nylon-bag method, examining eight kinds of roughage. All the experimental roughage types were analyzed for the effective degradability (ED) of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL) and hemicellulose (HC) fractions. Then, prediction models for the roughage fiber degradation were established based on the characteristics of lignin monomer content. The results showed that S, S/G and S/(G+S+H) were positively correlated with the ED of NDF, ADF, CEL and HC; H/S was negatively correlated. For the effective degradability of ADL (ADLD), S and S/(G+S+H) were positively correlated with it; H, H/G, H/S and H/(G+S+H) were negatively correlated. The model with the highest fitting degree was ADLD = 0.161 − 1.918 × H + 3.152 × S (R<sup>2</sup> = 0.758, <i>p</i> < 0.01). These results indicated that the lignin monomer composition is closely related to the utilization rate of roughage fiber. S-type lignin monomer plays a vital role in the fiber degradation of roughage. The experiment found the effect of lignin monomer composition on the degradation of fiber fractions using buffalo as the experimental animal and constructed prediction models, providing a scientific basis for building a new technological method using lignin composition to evaluate buffalo roughage. Furthermore, the capacity of ADL degradation of buffalo was proved in this experiment. In order to further explore the ability of lignin degradation by the buffalo, the DNA of rumen microorganisms was extracted for sequencing. The top three composition of rumen microorganisms at the genus level were Prevotella_1, 226, Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG-011. Six strains with lignin degradation ability were screened from buffalo rumen contents. This experiment also revealed that the buffalos possess rumen microorganisms with lignin degradation potential.https://www.mdpi.com/2077-0472/11/10/1015lignin monomerroughagedegradationbuffalorumen microorganisms |
| spellingShingle | Huimin Zhong Jiayan Zhou Mohamed Abdelrahman Hao Xu Zian Wu Luncheng Cui Zhenhua Ma Liguo Yang Xiang Li The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) Agriculture lignin monomer roughage degradation buffalo rumen microorganisms |
| title | The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) |
| title_full | The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) |
| title_fullStr | The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) |
| title_full_unstemmed | The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) |
| title_short | The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (<i>Bubalus bubalis</i>) |
| title_sort | effect of lignin composition on ruminal fiber fractions degradation from different roughage sources in water buffalo i bubalus bubalis i |
| topic | lignin monomer roughage degradation buffalo rumen microorganisms |
| url | https://www.mdpi.com/2077-0472/11/10/1015 |
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