16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients
Background and objectives: Elevated levels of 16α-hydroxyestrone (16α-OHE1) have been linked to increased risk of prostate cancer (PC) and estrogen receptor (ER) had been expressed in prostate tissue but the combined effect of 16α-OHE1 and ER (α) is lacking. We investigated the binding specifici...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MRE Press
2021-09-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/jomh/article/20210928-454/pdf/JOMH2021041201.pdf |
| Summary: | Background and objectives: Elevated levels of
16α-hydroxyestrone (16α-OHE1) have been linked to
increased risk of prostate cancer (PC) and estrogen receptor (ER) had been
expressed in prostate tissue but the combined effect of 16α-OHE1
and ER (α) is lacking. We investigated the binding specificity
of antibodies from PC with 16α-OHE1-ER complex in the sera
from PC patients.
Materials and methods: The antibodies in the serum from 60 PC
patients and 40 control subjects were evaluated from ELISA (direct binding and
competition) and quantitative precipitin titration. Competition ELISA was also
used to estimates 16α-OHE1 concentration and
2-hydroxyestrone (2-OHE1)/16α-OHE1 ratio in PC patients.
Results: Antibodies from PC patients demonstrate high binding
to 16α-OHE1-ER in comparison to ER (p < 0.05) or
16α-OHE1 (p < 0.001). The relative affinity of PC IgG
was found to be high for 16α-OHE1-ER (1.19 × 10-7 M)
as compared to ER (1.45 × 10-6 M) or 16α-OHE1 (1.13
× 10-6 M).
Conclusion: High affinity of PC IgG with 16α-OHE1-ER
might explain the possible antigenic role and 16α-OHE1-ER acted as
high affinity antigen for antibodies from PC. The interaction between
16α-OHE1 and ER makes a complex in the prostate tissues
and this may generate antibodies against this complex in the cancer patients. |
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| ISSN: | 1875-6859 |