16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients
Background and objectives: Elevated levels of 16α-hydroxyestrone (16α-OHE1) have been linked to increased risk of prostate cancer (PC) and estrogen receptor (ER) had been expressed in prostate tissue but the combined effect of 16α-OHE1 and ER (α) is lacking. We investigated the binding specifici...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MRE Press
2021-09-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/jomh/article/20210928-454/pdf/JOMH2021041201.pdf |
| _version_ | 1797327162087309312 |
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| author | Wahid Ali Khan Mohd. Wajid Ali Khan |
| author_facet | Wahid Ali Khan Mohd. Wajid Ali Khan |
| author_sort | Wahid Ali Khan |
| collection | DOAJ |
| description | Background and objectives: Elevated levels of
16α-hydroxyestrone (16α-OHE1) have been linked to
increased risk of prostate cancer (PC) and estrogen receptor (ER) had been
expressed in prostate tissue but the combined effect of 16α-OHE1
and ER (α) is lacking. We investigated the binding specificity
of antibodies from PC with 16α-OHE1-ER complex in the sera
from PC patients.
Materials and methods: The antibodies in the serum from 60 PC
patients and 40 control subjects were evaluated from ELISA (direct binding and
competition) and quantitative precipitin titration. Competition ELISA was also
used to estimates 16α-OHE1 concentration and
2-hydroxyestrone (2-OHE1)/16α-OHE1 ratio in PC patients.
Results: Antibodies from PC patients demonstrate high binding
to 16α-OHE1-ER in comparison to ER (p < 0.05) or
16α-OHE1 (p < 0.001). The relative affinity of PC IgG
was found to be high for 16α-OHE1-ER (1.19 × 10-7 M)
as compared to ER (1.45 × 10-6 M) or 16α-OHE1 (1.13
× 10-6 M).
Conclusion: High affinity of PC IgG with 16α-OHE1-ER
might explain the possible antigenic role and 16α-OHE1-ER acted as
high affinity antigen for antibodies from PC. The interaction between
16α-OHE1 and ER makes a complex in the prostate tissues
and this may generate antibodies against this complex in the cancer patients. |
| first_indexed | 2024-03-08T06:34:28Z |
| format | Article |
| id | doaj.art-982d6d94bf0444809ee074622ba3828c |
| institution | Directory Open Access Journal |
| issn | 1875-6859 |
| language | English |
| last_indexed | 2024-03-08T06:34:28Z |
| publishDate | 2021-09-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj.art-982d6d94bf0444809ee074622ba3828c2024-02-03T10:53:25ZengMRE PressJournal of Men's Health1875-68592021-09-0117411712710.31083/jomh.2021.103S1875-6867(21)00405-X16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patientsWahid Ali Khan0Mohd. Wajid Ali Khan1Department of Clinical Biochemistry, College of Medicine, King Khalid University, 960 Abha, Kingdom of Saudi ArabiaDepartment of Chemistry, College of Science, University of Hail, 2440 Hail, Kingdom of Saudi ArabiaBackground and objectives: Elevated levels of 16α-hydroxyestrone (16α-OHE1) have been linked to increased risk of prostate cancer (PC) and estrogen receptor (ER) had been expressed in prostate tissue but the combined effect of 16α-OHE1 and ER (α) is lacking. We investigated the binding specificity of antibodies from PC with 16α-OHE1-ER complex in the sera from PC patients. Materials and methods: The antibodies in the serum from 60 PC patients and 40 control subjects were evaluated from ELISA (direct binding and competition) and quantitative precipitin titration. Competition ELISA was also used to estimates 16α-OHE1 concentration and 2-hydroxyestrone (2-OHE1)/16α-OHE1 ratio in PC patients. Results: Antibodies from PC patients demonstrate high binding to 16α-OHE1-ER in comparison to ER (p < 0.05) or 16α-OHE1 (p < 0.001). The relative affinity of PC IgG was found to be high for 16α-OHE1-ER (1.19 × 10-7 M) as compared to ER (1.45 × 10-6 M) or 16α-OHE1 (1.13 × 10-6 M). Conclusion: High affinity of PC IgG with 16α-OHE1-ER might explain the possible antigenic role and 16α-OHE1-ER acted as high affinity antigen for antibodies from PC. The interaction between 16α-OHE1 and ER makes a complex in the prostate tissues and this may generate antibodies against this complex in the cancer patients.https://oss.jomh.org/jomh/article/20210928-454/pdf/JOMH2021041201.pdfprostate cancer16α-hydroxyestroneestrogen receptorelisaantibodies |
| spellingShingle | Wahid Ali Khan Mohd. Wajid Ali Khan 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients Journal of Men's Health prostate cancer 16α-hydroxyestrone estrogen receptor elisa antibodies |
| title | 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients |
| title_full | 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients |
| title_fullStr | 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients |
| title_full_unstemmed | 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients |
| title_short | 16𝜶-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients |
| title_sort | 16𝜶 hydroxyestrone and its receptor complex high affinity antigen for antibodies from prostate cancer patients |
| topic | prostate cancer 16α-hydroxyestrone estrogen receptor elisa antibodies |
| url | https://oss.jomh.org/jomh/article/20210928-454/pdf/JOMH2021041201.pdf |
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