Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers
Abstract Background Salmonella Enteritidis (SE) propagates in chickens' gastrointestinal surfaces and is transmitted to humans, causing food poisoning. Oral supplementation with natural nanoparticles can overcome the harsh gastrointestinal conditions facing oral vaccines and requires no antibio...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-023-03632-1 |
| _version_ | 1797752706100625408 |
|---|---|
| author | Adel Attia M. Ahmad Gamal A. Elmowalid Ahmed E. Abdelhamid Alaa Abed Elrazak Mohammad Ashraf M. O. Abdelwahab |
| author_facet | Adel Attia M. Ahmad Gamal A. Elmowalid Ahmed E. Abdelhamid Alaa Abed Elrazak Mohammad Ashraf M. O. Abdelwahab |
| author_sort | Adel Attia M. Ahmad |
| collection | DOAJ |
| description | Abstract Background Salmonella Enteritidis (SE) propagates in chickens' gastrointestinal surfaces and is transmitted to humans, causing food poisoning. Oral supplementation with natural nanoparticles can overcome the harsh gastrointestinal conditions facing oral vaccines and requires no antibiotic administration to protect against microbial infection. This study was designed to study Nigella sativa-chitosan nanoparticles (CNP-NS) prophylactic immunomodulatory efficacy against SE infection in broiler chicks. The CNP-NS was prepared and characterized, and its in vivo immunomodulatory activities against an avian virulent-MDR SE-induced challenge in chicks were investigated. Result To verify the immune-protective activities of the CNP-NS, colony forming units (CFU) in the liver and fecal droppings; intestinal histopathological alterations and immune cell recruitment; MUC-2, TLR-4, cecal cytokines, and specific IgA gene expression levels were assessed. On the 7th and 12th days after the SE challenge, the CNP-NS supplemented chicks showed complete clearance of SE CFU in livers and fecal droppings, as well as an improvement in food conversion rate compared to non-supplemented CNP-NS that revealed the presence of the challenge SE CFU on the same days. A prominent influx of antigen presenting cells and lymphoid aggregates into the intestinal wall, spleen, and liver was detected with improvements in the intestinal villi morphometry of the CNP-NS-supplemented chicks. The changes of INF-γ, IL-1β, and IL-4 cecal cytokines, as well as TLR-4, MUC-2, and IgA mRNA expression levels, confirm CNP-NS immunomodulatory activities and provide a mechanism(s) for its protective actions against the induced SE challenge of the tested chickens. Conclusion These findings suggest promising useful insights into CNP-NS supplementation as a safe food additive for poultry meat consumers' and a protective immunomodulator of the chickens' mucosal immune systems. It could be recommended for epidemiological purposes to reduce the risk of SE food poisoning and transmission to humans. |
| first_indexed | 2024-03-12T17:08:25Z |
| format | Article |
| id | doaj.art-98310e3f27204a8e9d1efc1bff76ebc8 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-12T17:08:25Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-98310e3f27204a8e9d1efc1bff76ebc82023-08-06T11:17:16ZengBMCBMC Veterinary Research1746-61482023-08-0119111610.1186/s12917-023-03632-1Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilersAdel Attia M. Ahmad0Gamal A. Elmowalid1Ahmed E. Abdelhamid2Alaa Abed Elrazak Mohammad3Ashraf M. O. Abdelwahab4Department of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityPolymers and Pigments Department, National Research CentreFaculty of Veterinary Medicine, Zagazig UniversityDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityAbstract Background Salmonella Enteritidis (SE) propagates in chickens' gastrointestinal surfaces and is transmitted to humans, causing food poisoning. Oral supplementation with natural nanoparticles can overcome the harsh gastrointestinal conditions facing oral vaccines and requires no antibiotic administration to protect against microbial infection. This study was designed to study Nigella sativa-chitosan nanoparticles (CNP-NS) prophylactic immunomodulatory efficacy against SE infection in broiler chicks. The CNP-NS was prepared and characterized, and its in vivo immunomodulatory activities against an avian virulent-MDR SE-induced challenge in chicks were investigated. Result To verify the immune-protective activities of the CNP-NS, colony forming units (CFU) in the liver and fecal droppings; intestinal histopathological alterations and immune cell recruitment; MUC-2, TLR-4, cecal cytokines, and specific IgA gene expression levels were assessed. On the 7th and 12th days after the SE challenge, the CNP-NS supplemented chicks showed complete clearance of SE CFU in livers and fecal droppings, as well as an improvement in food conversion rate compared to non-supplemented CNP-NS that revealed the presence of the challenge SE CFU on the same days. A prominent influx of antigen presenting cells and lymphoid aggregates into the intestinal wall, spleen, and liver was detected with improvements in the intestinal villi morphometry of the CNP-NS-supplemented chicks. The changes of INF-γ, IL-1β, and IL-4 cecal cytokines, as well as TLR-4, MUC-2, and IgA mRNA expression levels, confirm CNP-NS immunomodulatory activities and provide a mechanism(s) for its protective actions against the induced SE challenge of the tested chickens. Conclusion These findings suggest promising useful insights into CNP-NS supplementation as a safe food additive for poultry meat consumers' and a protective immunomodulator of the chickens' mucosal immune systems. It could be recommended for epidemiological purposes to reduce the risk of SE food poisoning and transmission to humans.https://doi.org/10.1186/s12917-023-03632-1NanoparticlesChitosanNigella sativaImmunomodulationSalmonella EnteritidisFood-borne diseases |
| spellingShingle | Adel Attia M. Ahmad Gamal A. Elmowalid Ahmed E. Abdelhamid Alaa Abed Elrazak Mohammad Ashraf M. O. Abdelwahab Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers BMC Veterinary Research Nanoparticles Chitosan Nigella sativa Immunomodulation Salmonella Enteritidis Food-borne diseases |
| title | Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers |
| title_full | Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers |
| title_fullStr | Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers |
| title_full_unstemmed | Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers |
| title_short | Nigella sativa-chitosan nanoparticles: Novel intestinal mucosal immunomodulator controls and protects against Salmonella enterica serovar Enteritidis infection in broilers |
| title_sort | nigella sativa chitosan nanoparticles novel intestinal mucosal immunomodulator controls and protects against salmonella enterica serovar enteritidis infection in broilers |
| topic | Nanoparticles Chitosan Nigella sativa Immunomodulation Salmonella Enteritidis Food-borne diseases |
| url | https://doi.org/10.1186/s12917-023-03632-1 |
| work_keys_str_mv | AT adelattiamahmad nigellasativachitosannanoparticlesnovelintestinalmucosalimmunomodulatorcontrolsandprotectsagainstsalmonellaentericaserovarenteritidisinfectioninbroilers AT gamalaelmowalid nigellasativachitosannanoparticlesnovelintestinalmucosalimmunomodulatorcontrolsandprotectsagainstsalmonellaentericaserovarenteritidisinfectioninbroilers AT ahmedeabdelhamid nigellasativachitosannanoparticlesnovelintestinalmucosalimmunomodulatorcontrolsandprotectsagainstsalmonellaentericaserovarenteritidisinfectioninbroilers AT alaaabedelrazakmohammad nigellasativachitosannanoparticlesnovelintestinalmucosalimmunomodulatorcontrolsandprotectsagainstsalmonellaentericaserovarenteritidisinfectioninbroilers AT ashrafmoabdelwahab nigellasativachitosannanoparticlesnovelintestinalmucosalimmunomodulatorcontrolsandprotectsagainstsalmonellaentericaserovarenteritidisinfectioninbroilers |