Missing effects of zinc in a porcine model of recurrent endotoxemia
<p>Abstract</p> <p>Background</p> <p>Chronic human sepsis often is characterised by the compensatory anti-inflammatory response syndrome (CARS). During CARS, anti-inflammatory cytokines depress the inflammatory response leading to secondary and opportunistic infections....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-10-01
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| Series: | BMC Surgery |
| Online Access: | http://www.biomedcentral.com/1471-2482/5/22 |
| _version_ | 1828294499127787520 |
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| author | Klinge Uwe Stumpf Michael Anurov Michael Klosterhalfen Bernd Krones Carsten J Oettinger Alexander P Schumpelick Volker |
| author_facet | Klinge Uwe Stumpf Michael Anurov Michael Klosterhalfen Bernd Krones Carsten J Oettinger Alexander P Schumpelick Volker |
| author_sort | Klinge Uwe |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Chronic human sepsis often is characterised by the compensatory anti-inflammatory response syndrome (CARS). During CARS, anti-inflammatory cytokines depress the inflammatory response leading to secondary and opportunistic infections. Proved <it>in vitro </it>as well as <it>in vivo</it>, zinc's pro-inflammatory effect might overcome this depression.</p> <p>Methods</p> <p>We used the model of porcine LPS-induced endotoxemia established by Klosterhalfen et al. 10 pigs were divided into two groups (n = 5). Endotoxemia was induced by recurrent intravenous LPS-application (1.0 μg/kg E. coli WO 111:B4) at hours 0, 5, and 12. At hour 10, each group received an intravenous treatment (group I = saline, group II = 5.0 mg/kg elementary zinc). Monitoring included hemodynamics, blood gas analysis, and the thermal dilution technique for the measurement of extravascular lung water and intrapulmonary shunt. Plasma concentrations of IL-6 and TNF-alpha were measured by ELISA. Morphology included weight of the lungs, width of the alveolar septae, and rate of paracentral liver necrosis.</p> <p>Results</p> <p>Zinc's application only trended to partly improve the pulmonary function. Compared to saline, significant differences were very rare. IL-6 and TNF-alpha were predominately measured higher in the zinc group. Again, significance was only reached sporadically. Hemodynamics and morphology revealed no significant differences at all.</p> <p>Conclusion</p> <p>The application of zinc in this model of recurrent endotoxemia is feasible and without harmful effects. However, a protection or restoration of clinical relevance is not evident in our setting. The pulmonary function just trends to improve, cytokine liberation is only partly activated, hemodynamics and morphology were not influenced. Further pre-clinical studies have to define zinc's role as a therapeutic tool during CARS.</p> |
| first_indexed | 2024-04-13T11:38:40Z |
| format | Article |
| id | doaj.art-9831696f7ebd4e52b49750edb5386542 |
| institution | Directory Open Access Journal |
| issn | 1471-2482 |
| language | English |
| last_indexed | 2024-04-13T11:38:40Z |
| publishDate | 2005-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Surgery |
| spelling | doaj.art-9831696f7ebd4e52b49750edb53865422022-12-22T02:48:21ZengBMCBMC Surgery1471-24822005-10-01512210.1186/1471-2482-5-22Missing effects of zinc in a porcine model of recurrent endotoxemiaKlinge UweStumpf MichaelAnurov MichaelKlosterhalfen BerndKrones Carsten JOettinger Alexander PSchumpelick Volker<p>Abstract</p> <p>Background</p> <p>Chronic human sepsis often is characterised by the compensatory anti-inflammatory response syndrome (CARS). During CARS, anti-inflammatory cytokines depress the inflammatory response leading to secondary and opportunistic infections. Proved <it>in vitro </it>as well as <it>in vivo</it>, zinc's pro-inflammatory effect might overcome this depression.</p> <p>Methods</p> <p>We used the model of porcine LPS-induced endotoxemia established by Klosterhalfen et al. 10 pigs were divided into two groups (n = 5). Endotoxemia was induced by recurrent intravenous LPS-application (1.0 μg/kg E. coli WO 111:B4) at hours 0, 5, and 12. At hour 10, each group received an intravenous treatment (group I = saline, group II = 5.0 mg/kg elementary zinc). Monitoring included hemodynamics, blood gas analysis, and the thermal dilution technique for the measurement of extravascular lung water and intrapulmonary shunt. Plasma concentrations of IL-6 and TNF-alpha were measured by ELISA. Morphology included weight of the lungs, width of the alveolar septae, and rate of paracentral liver necrosis.</p> <p>Results</p> <p>Zinc's application only trended to partly improve the pulmonary function. Compared to saline, significant differences were very rare. IL-6 and TNF-alpha were predominately measured higher in the zinc group. Again, significance was only reached sporadically. Hemodynamics and morphology revealed no significant differences at all.</p> <p>Conclusion</p> <p>The application of zinc in this model of recurrent endotoxemia is feasible and without harmful effects. However, a protection or restoration of clinical relevance is not evident in our setting. The pulmonary function just trends to improve, cytokine liberation is only partly activated, hemodynamics and morphology were not influenced. Further pre-clinical studies have to define zinc's role as a therapeutic tool during CARS.</p>http://www.biomedcentral.com/1471-2482/5/22 |
| spellingShingle | Klinge Uwe Stumpf Michael Anurov Michael Klosterhalfen Bernd Krones Carsten J Oettinger Alexander P Schumpelick Volker Missing effects of zinc in a porcine model of recurrent endotoxemia BMC Surgery |
| title | Missing effects of zinc in a porcine model of recurrent endotoxemia |
| title_full | Missing effects of zinc in a porcine model of recurrent endotoxemia |
| title_fullStr | Missing effects of zinc in a porcine model of recurrent endotoxemia |
| title_full_unstemmed | Missing effects of zinc in a porcine model of recurrent endotoxemia |
| title_short | Missing effects of zinc in a porcine model of recurrent endotoxemia |
| title_sort | missing effects of zinc in a porcine model of recurrent endotoxemia |
| url | http://www.biomedcentral.com/1471-2482/5/22 |
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