Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry
Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) on a reverse...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-10-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383514000525 |
| _version_ | 1818465954191900672 |
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| author | Zhongliang Zhang Qiang Li Qiufen Li Simiao Du Yongquan Zhou Chunming Lv Yan Zhao Yongjun Wang Ning Zhang |
| author_facet | Zhongliang Zhang Qiang Li Qiufen Li Simiao Du Yongquan Zhou Chunming Lv Yan Zhao Yongjun Wang Ning Zhang |
| author_sort | Zhongliang Zhang |
| collection | DOAJ |
| description | Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components. |
| first_indexed | 2024-04-13T20:52:53Z |
| format | Article |
| id | doaj.art-98321656eaf641e18d7334f1215e3775 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-04-13T20:52:53Z |
| publishDate | 2014-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-98321656eaf641e18d7334f1215e37752022-12-22T02:30:27ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432014-10-014538439310.1016/j.apsb.2014.05.003Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometryZhongliang Zhang0Qiang Li1Qiufen Li2Simiao Du3Yongquan Zhou4Chunming Lv5Yan Zhao6Yongjun Wang7Ning Zhang8Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaInstitute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaExperimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaQi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components.http://www.sciencedirect.com/science/article/pii/S2211383514000525UHPLC–MS/MSActive componentsTraditional Chinese medicineQi She Pill (QSP) |
| spellingShingle | Zhongliang Zhang Qiang Li Qiufen Li Simiao Du Yongquan Zhou Chunming Lv Yan Zhao Yongjun Wang Ning Zhang Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry Acta Pharmaceutica Sinica B UHPLC–MS/MS Active components Traditional Chinese medicine Qi She Pill (QSP) |
| title | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title_full | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title_fullStr | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title_full_unstemmed | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title_short | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| title_sort | simultaneous determination of nineteen major components in qi she pill by ultra high performance liquid chromatography tandem mass spectrometry |
| topic | UHPLC–MS/MS Active components Traditional Chinese medicine Qi She Pill (QSP) |
| url | http://www.sciencedirect.com/science/article/pii/S2211383514000525 |
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