The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis
Hexokinase (HXK) is involved in hexose phosphorylation, sugar sensing, and signal transduction, all of which regulate plant growth and adaptation to stresses. Gossypium hirsutum L. is one of the most important fiber crops in the world, however, little is known about the HXKs gene family in G. hirsut...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.882587/full |
| _version_ | 1828365179737341952 |
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| author | Lingling Dou Zihan Li Huiqin Wang HuaiZhu Li Guanghui Xiao Xianliang Zhang |
| author_facet | Lingling Dou Zihan Li Huiqin Wang HuaiZhu Li Guanghui Xiao Xianliang Zhang |
| author_sort | Lingling Dou |
| collection | DOAJ |
| description | Hexokinase (HXK) is involved in hexose phosphorylation, sugar sensing, and signal transduction, all of which regulate plant growth and adaptation to stresses. Gossypium hirsutum L. is one of the most important fiber crops in the world, however, little is known about the HXKs gene family in G. hirsutum L. We identified 17 GhHXKs from the allotetraploid G. hirsutum L. genome (AADD). G. raimondii (DD) and G. arboreum (AA) are the diploid progenitors of G. hirsutum L. and contributed equally to the At_genome and Dt_genome GhHXKs genes. The chromosomal locations and exon-intron structures of GhHXK genes among cotton species are conservative. Phylogenetic analysis grouped the HXK proteins into four and three groups based on whether they were monocotyledons and dicotyledons, respectively. Duplication event analysis demonstrated that HXKs in G. hirsutum L. primarily originated from segmental duplication, which prior to diploid hybridization. Experiments of qRT-PCR, transcriptome and promoter cis-elements demonstrated that GhHXKs’ promoters have auxin and GA responsive elements that are highly expressed in the fiber initiation and elongation stages, while the promoters contain ABA-, MeJA-, and SA-responsive elements that are highly expressed during the synthesis of the secondary cell wall. We performed a comprehensive analysis of the GhHXK gene family is a vital fiber crop, which lays the foundation for future studies assessing its role in fiber development. |
| first_indexed | 2024-04-14T05:27:23Z |
| format | Article |
| id | doaj.art-98347550818f468b9c33e6e920671156 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T05:27:23Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-98347550818f468b9c33e6e9206711562022-12-22T02:09:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.882587882587The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics AnalysisLingling Dou0Zihan Li1Huiqin Wang2HuaiZhu Li3Guanghui Xiao4Xianliang Zhang5School of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an, ChinaSchool of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang, ChinaCollege of Life Sciences, Shaanxi Normal University, Xi’an, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaHexokinase (HXK) is involved in hexose phosphorylation, sugar sensing, and signal transduction, all of which regulate plant growth and adaptation to stresses. Gossypium hirsutum L. is one of the most important fiber crops in the world, however, little is known about the HXKs gene family in G. hirsutum L. We identified 17 GhHXKs from the allotetraploid G. hirsutum L. genome (AADD). G. raimondii (DD) and G. arboreum (AA) are the diploid progenitors of G. hirsutum L. and contributed equally to the At_genome and Dt_genome GhHXKs genes. The chromosomal locations and exon-intron structures of GhHXK genes among cotton species are conservative. Phylogenetic analysis grouped the HXK proteins into four and three groups based on whether they were monocotyledons and dicotyledons, respectively. Duplication event analysis demonstrated that HXKs in G. hirsutum L. primarily originated from segmental duplication, which prior to diploid hybridization. Experiments of qRT-PCR, transcriptome and promoter cis-elements demonstrated that GhHXKs’ promoters have auxin and GA responsive elements that are highly expressed in the fiber initiation and elongation stages, while the promoters contain ABA-, MeJA-, and SA-responsive elements that are highly expressed during the synthesis of the secondary cell wall. We performed a comprehensive analysis of the GhHXK gene family is a vital fiber crop, which lays the foundation for future studies assessing its role in fiber development.https://www.frontiersin.org/articles/10.3389/fpls.2022.882587/fullGossypium hirsutumhexokinasesequence analysisthe evolutionaryexpression pattern |
| spellingShingle | Lingling Dou Zihan Li Huiqin Wang HuaiZhu Li Guanghui Xiao Xianliang Zhang The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis Frontiers in Plant Science Gossypium hirsutum hexokinase sequence analysis the evolutionary expression pattern |
| title | The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis |
| title_full | The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis |
| title_fullStr | The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis |
| title_full_unstemmed | The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis |
| title_short | The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis |
| title_sort | hexokinase gene family in cotton genome wide characterization and bioinformatics analysis |
| topic | Gossypium hirsutum hexokinase sequence analysis the evolutionary expression pattern |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.882587/full |
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