Protease Produced by Endophytic Fungi: A Systematic Review
The purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit the production, characterization, and purification of proteases from endophytic fung...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/22/7062 |
| _version_ | 1797509165646610432 |
|---|---|
| author | Victor Hugo Souto Bezerra Samuel Leite Cardoso Yris Fonseca-Bazzo Dâmaris Silveira Pérola Oliveira Magalhães Paula Monteiro Souza |
| author_facet | Victor Hugo Souto Bezerra Samuel Leite Cardoso Yris Fonseca-Bazzo Dâmaris Silveira Pérola Oliveira Magalhães Paula Monteiro Souza |
| author_sort | Victor Hugo Souto Bezerra |
| collection | DOAJ |
| description | The purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit the production, characterization, and purification of proteases from endophytic fungi. This study followed the PRISMA, and the search was conducted on five databases: PubMed, PMC, Science Direct, Scopus Articles, and Web of Science up until 18 May 2021, with no time or language restrictions. The methodology of the selected studies was evaluated using GRADE. Protease production, optimization, purification, and characterization were the main evaluated outcomes. Of the 5540 initially gathered studies, 15 met the inclusion criteria after a two-step selection process. Only two studies optimized the protease production using statistical design and two reported enzyme purification and characterization. The genus <i>Penicillium</i> and <i>Aspergillus</i> were the most cited among the eleven different genera of endophytic fungi evaluated in the selected articles. Six studies proved the ability of some endophytic fungi to produce fibrinolytic proteases, demonstrating that endophytic fungi can be exploited for the further production of agents used in thrombolytic therapy. However, further characterization and physicochemical studies are required to evaluate the real potential of endophytic fungi as sources of industrial enzymes. |
| first_indexed | 2024-03-10T05:13:43Z |
| format | Article |
| id | doaj.art-9836993682764e338998211090021fe0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:13:43Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9836993682764e338998211090021fe02023-11-23T00:37:58ZengMDPI AGMolecules1420-30492021-11-012622706210.3390/molecules26227062Protease Produced by Endophytic Fungi: A Systematic ReviewVictor Hugo Souto Bezerra0Samuel Leite Cardoso1Yris Fonseca-Bazzo2Dâmaris Silveira3Pérola Oliveira Magalhães4Paula Monteiro Souza5Natural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilNatural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilNatural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilNatural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilNatural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilNatural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, BrazilThe purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit the production, characterization, and purification of proteases from endophytic fungi. This study followed the PRISMA, and the search was conducted on five databases: PubMed, PMC, Science Direct, Scopus Articles, and Web of Science up until 18 May 2021, with no time or language restrictions. The methodology of the selected studies was evaluated using GRADE. Protease production, optimization, purification, and characterization were the main evaluated outcomes. Of the 5540 initially gathered studies, 15 met the inclusion criteria after a two-step selection process. Only two studies optimized the protease production using statistical design and two reported enzyme purification and characterization. The genus <i>Penicillium</i> and <i>Aspergillus</i> were the most cited among the eleven different genera of endophytic fungi evaluated in the selected articles. Six studies proved the ability of some endophytic fungi to produce fibrinolytic proteases, demonstrating that endophytic fungi can be exploited for the further production of agents used in thrombolytic therapy. However, further characterization and physicochemical studies are required to evaluate the real potential of endophytic fungi as sources of industrial enzymes.https://www.mdpi.com/1420-3049/26/22/7062proteaseendophytic fungisystematic review |
| spellingShingle | Victor Hugo Souto Bezerra Samuel Leite Cardoso Yris Fonseca-Bazzo Dâmaris Silveira Pérola Oliveira Magalhães Paula Monteiro Souza Protease Produced by Endophytic Fungi: A Systematic Review Molecules protease endophytic fungi systematic review |
| title | Protease Produced by Endophytic Fungi: A Systematic Review |
| title_full | Protease Produced by Endophytic Fungi: A Systematic Review |
| title_fullStr | Protease Produced by Endophytic Fungi: A Systematic Review |
| title_full_unstemmed | Protease Produced by Endophytic Fungi: A Systematic Review |
| title_short | Protease Produced by Endophytic Fungi: A Systematic Review |
| title_sort | protease produced by endophytic fungi a systematic review |
| topic | protease endophytic fungi systematic review |
| url | https://www.mdpi.com/1420-3049/26/22/7062 |
| work_keys_str_mv | AT victorhugosoutobezerra proteaseproducedbyendophyticfungiasystematicreview AT samuelleitecardoso proteaseproducedbyendophyticfungiasystematicreview AT yrisfonsecabazzo proteaseproducedbyendophyticfungiasystematicreview AT damarissilveira proteaseproducedbyendophyticfungiasystematicreview AT perolaoliveiramagalhaes proteaseproducedbyendophyticfungiasystematicreview AT paulamonteirosouza proteaseproducedbyendophyticfungiasystematicreview |