Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies
Phytochemical study of <i>Chiliadenus montanus</i> aerial parts afforded six compounds; Intermedeol (1), 5α-hydroperoxy-β-eudesmol (2), 5,7-dihydroxy-3,3’,4’-trimethoxyflavone (3), 5,7,4’-trihydroxy-3,6,3’-trimethoxyflavone (jaceidin) (4), eudesm-11,13-ene-1β,4β,7α-triol (5) and 1β,4β,7β...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/1031 |
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| author | Sameh S. Elhady Enas E. Eltamany Amera E. Shaaban Alaa A. Bagalagel Yosra A. Muhammad Norhan M. El-Sayed Seif N. Ayyad Amal A. M. Ahmed Mohamed S. Elgawish Safwat A. Ahmed |
| author_facet | Sameh S. Elhady Enas E. Eltamany Amera E. Shaaban Alaa A. Bagalagel Yosra A. Muhammad Norhan M. El-Sayed Seif N. Ayyad Amal A. M. Ahmed Mohamed S. Elgawish Safwat A. Ahmed |
| author_sort | Sameh S. Elhady |
| collection | DOAJ |
| description | Phytochemical study of <i>Chiliadenus montanus</i> aerial parts afforded six compounds; Intermedeol (1), 5α-hydroperoxy-β-eudesmol (2), 5,7-dihydroxy-3,3’,4’-trimethoxyflavone (3), 5,7,4’-trihydroxy-3,6,3’-trimethoxyflavone (jaceidin) (4), eudesm-11,13-ene-1β,4β,7α-triol (5) and 1β,4β,7β,11-tetrahydroxyeudesmane (6). These compounds were identified based on their NMR spectral data. The isolated compounds were tested for their cytotoxicity against liver cancer cell line (HepG2) and breast cancer cell line (MCF-7). Jaceidin flavonoid (4) exhibited the highest cytotoxic effect in vitro. Therefore, both of jaceidin and <i>C. montanus</i> extract were evaluated for their in vivo anti-tumor activity against Ehrlich’s ascites carcinoma (EAC). Compared to control group, jaceidin and <i>C. montanus</i> extract decreased the tumor weight, improved the histological picture of tumor cells, lowered the levels of VEGF and ameliorate the oxidative stress. Molecular docking and in silico studies suggested that jaceidin was a selective inhibitor of VEGF-mediated angiogenesis with excellent membrane permeability and oral bioavailability. |
| first_indexed | 2024-03-10T17:26:34Z |
| format | Article |
| id | doaj.art-98388bdeb9474167b51209195e0c0299 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T17:26:34Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-98388bdeb9474167b51209195e0c02992023-11-20T10:09:54ZengMDPI AGPlants2223-77472020-08-0198103110.3390/plants9081031Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico StudiesSameh S. Elhady0Enas E. Eltamany1Amera E. Shaaban2Alaa A. Bagalagel3Yosra A. Muhammad4Norhan M. El-Sayed5Seif N. Ayyad6Amal A. M. Ahmed7Mohamed S. Elgawish8Safwat A. Ahmed9Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, 41522 Ismailia, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, 41522 Ismailia, EgyptDepartment of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmacology & Toxicology, Faculty of Pharmacy, Suez Canal University, 41522 Ismailia, EgyptDepartment of Organic Chemistry, Faculty of Science, Damietta University, New Damietta 34511, EgyptDepartment of Cytology & Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Medicinal Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, 41522 Ismailia, EgyptPhytochemical study of <i>Chiliadenus montanus</i> aerial parts afforded six compounds; Intermedeol (1), 5α-hydroperoxy-β-eudesmol (2), 5,7-dihydroxy-3,3’,4’-trimethoxyflavone (3), 5,7,4’-trihydroxy-3,6,3’-trimethoxyflavone (jaceidin) (4), eudesm-11,13-ene-1β,4β,7α-triol (5) and 1β,4β,7β,11-tetrahydroxyeudesmane (6). These compounds were identified based on their NMR spectral data. The isolated compounds were tested for their cytotoxicity against liver cancer cell line (HepG2) and breast cancer cell line (MCF-7). Jaceidin flavonoid (4) exhibited the highest cytotoxic effect in vitro. Therefore, both of jaceidin and <i>C. montanus</i> extract were evaluated for their in vivo anti-tumor activity against Ehrlich’s ascites carcinoma (EAC). Compared to control group, jaceidin and <i>C. montanus</i> extract decreased the tumor weight, improved the histological picture of tumor cells, lowered the levels of VEGF and ameliorate the oxidative stress. Molecular docking and in silico studies suggested that jaceidin was a selective inhibitor of VEGF-mediated angiogenesis with excellent membrane permeability and oral bioavailability.https://www.mdpi.com/2223-7747/9/8/1031<i>Chiliadenus montanus</i>jaceidinanti-tumorEhrlich’s ascites carcinomaVEGF |
| spellingShingle | Sameh S. Elhady Enas E. Eltamany Amera E. Shaaban Alaa A. Bagalagel Yosra A. Muhammad Norhan M. El-Sayed Seif N. Ayyad Amal A. M. Ahmed Mohamed S. Elgawish Safwat A. Ahmed Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies Plants <i>Chiliadenus montanus</i> jaceidin anti-tumor Ehrlich’s ascites carcinoma VEGF |
| title | Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies |
| title_full | Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies |
| title_fullStr | Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies |
| title_full_unstemmed | Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies |
| title_short | Jaceidin Flavonoid Isolated from <i>Chiliadenus</i> <i>montanus</i> Attenuates Tumor Progression in Mice via VEGF Inhibition: In Vivo and In Silico Studies |
| title_sort | jaceidin flavonoid isolated from i chiliadenus i i montanus i attenuates tumor progression in mice via vegf inhibition in vivo and in silico studies |
| topic | <i>Chiliadenus montanus</i> jaceidin anti-tumor Ehrlich’s ascites carcinoma VEGF |
| url | https://www.mdpi.com/2223-7747/9/8/1031 |
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