Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study

Elderly subjects with more than 20 natural teeth have a higher healthy life expectancy than those with few or no teeth. The oral microbiome and its metabolome are associated with oral health, and they are also associated with systemic health via the oral-gut axis. Here, we analyzed the oral and gut...

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Main Authors: Yuichiro Nishimoto, Felix Salim, Kazuma Yama, Kota Kumagai, Ryutaro Jo, Minori Harada, Yuki Maruyama, Yuto Aita, Narumi Fujii, Takuya Inokuchi, Ryosuke Kawamata, Misato Sako, Yuko Ichiba, Kota Tsutsumi, Mitsuo Kimura, Yuka Mori, Shinnosuke Murakami, Yasushi Kakizawa, Takashi Kumagai, Shinji Fukuda
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-10-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233460/full
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author Yuichiro Nishimoto
Felix Salim
Felix Salim
Kazuma Yama
Kota Kumagai
Ryutaro Jo
Minori Harada
Yuki Maruyama
Yuto Aita
Narumi Fujii
Takuya Inokuchi
Ryosuke Kawamata
Misato Sako
Yuko Ichiba
Kota Tsutsumi
Mitsuo Kimura
Yuka Mori
Shinnosuke Murakami
Shinnosuke Murakami
Yasushi Kakizawa
Takashi Kumagai
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
author_facet Yuichiro Nishimoto
Felix Salim
Felix Salim
Kazuma Yama
Kota Kumagai
Ryutaro Jo
Minori Harada
Yuki Maruyama
Yuto Aita
Narumi Fujii
Takuya Inokuchi
Ryosuke Kawamata
Misato Sako
Yuko Ichiba
Kota Tsutsumi
Mitsuo Kimura
Yuka Mori
Shinnosuke Murakami
Shinnosuke Murakami
Yasushi Kakizawa
Takashi Kumagai
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
author_sort Yuichiro Nishimoto
collection DOAJ
description Elderly subjects with more than 20 natural teeth have a higher healthy life expectancy than those with few or no teeth. The oral microbiome and its metabolome are associated with oral health, and they are also associated with systemic health via the oral-gut axis. Here, we analyzed the oral and gut microbiome and metabolome profiles of elderly subjects with more than 26 natural teeth. Salivary samples collected as mouth-rinsed water and fecal samples were obtained from 22 healthy individuals, 10 elderly individuals with more than 26 natural teeth and 24 subjects with periodontal disease. The oral microbiome and metabolome profiles of elderly individuals resembled those of subjects with periodontal disease, with the metabolome showing a more substantial differential abundance of components. Despite the distinct oral metabolome profiles, there was no differential abundance of components in the gut microbiome and metabolomes, except for enrichment of short-chain fatty acids in elderly subjects. Finally, to investigate the relationship between the oral and gut microbiome and metabolome, we analyzed bacterial coexistence in the oral cavity and gut and analyzed the correlation of metabolite levels between the oral cavity and gut. However, there were few associations between oral and gut for bacteria and metabolites in either elderly or healthy subjects. Overall, these results indicate distinct oral microbiome and metabolome profiles, as well as the lack of an oral-gut axis in elderly subjects with a high number of natural teeth.
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spelling doaj.art-98394e6721554f4288af83c2c46c928a2023-10-12T09:15:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12334601233460Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot studyYuichiro Nishimoto0Felix Salim1Felix Salim2Kazuma Yama3Kota Kumagai4Ryutaro Jo5Minori Harada6Yuki Maruyama7Yuto Aita8Narumi Fujii9Takuya Inokuchi10Ryosuke Kawamata11Misato Sako12Yuko Ichiba13Kota Tsutsumi14Mitsuo Kimura15Yuka Mori16Shinnosuke Murakami17Shinnosuke Murakami18Yasushi Kakizawa19Takashi Kumagai20Shinji Fukuda21Shinji Fukuda22Shinji Fukuda23Shinji Fukuda24Shinji Fukuda25Metagen Inc., Tsuruoka, Yamagata, JapanMetagen Inc., Tsuruoka, Yamagata, JapanDepartment of Life Science and Technology, Tokyo Institute of Technology, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanHiyoshi Oral Health Clinics, Sakata, Yamagata, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanHiyoshi Oral Health Clinics, Sakata, Yamagata, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanMetagen Inc., Tsuruoka, Yamagata, JapanMetagen Inc., Tsuruoka, Yamagata, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, JapanResearch and Development Headquarters, Lion Corporation, Tokyo, JapanHiyoshi Oral Health Clinics, Sakata, Yamagata, JapanMetagen Inc., Tsuruoka, Yamagata, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, JapanLaboratory for Regenerative Microbiology, Juntendo University Graduate School of Medicine, Tokyo, JapanGut Environmental Design Group, Kanagawa Institute of Industrial Science and Technology, Kawasaki, Kanagawa, JapanTransborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, JapanElderly subjects with more than 20 natural teeth have a higher healthy life expectancy than those with few or no teeth. The oral microbiome and its metabolome are associated with oral health, and they are also associated with systemic health via the oral-gut axis. Here, we analyzed the oral and gut microbiome and metabolome profiles of elderly subjects with more than 26 natural teeth. Salivary samples collected as mouth-rinsed water and fecal samples were obtained from 22 healthy individuals, 10 elderly individuals with more than 26 natural teeth and 24 subjects with periodontal disease. The oral microbiome and metabolome profiles of elderly individuals resembled those of subjects with periodontal disease, with the metabolome showing a more substantial differential abundance of components. Despite the distinct oral metabolome profiles, there was no differential abundance of components in the gut microbiome and metabolomes, except for enrichment of short-chain fatty acids in elderly subjects. Finally, to investigate the relationship between the oral and gut microbiome and metabolome, we analyzed bacterial coexistence in the oral cavity and gut and analyzed the correlation of metabolite levels between the oral cavity and gut. However, there were few associations between oral and gut for bacteria and metabolites in either elderly or healthy subjects. Overall, these results indicate distinct oral microbiome and metabolome profiles, as well as the lack of an oral-gut axis in elderly subjects with a high number of natural teeth.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233460/fulloral microbiomeoral metabolomegut microbiomegut metabolomeoral-gut axis
spellingShingle Yuichiro Nishimoto
Felix Salim
Felix Salim
Kazuma Yama
Kota Kumagai
Ryutaro Jo
Minori Harada
Yuki Maruyama
Yuto Aita
Narumi Fujii
Takuya Inokuchi
Ryosuke Kawamata
Misato Sako
Yuko Ichiba
Kota Tsutsumi
Mitsuo Kimura
Yuka Mori
Shinnosuke Murakami
Shinnosuke Murakami
Yasushi Kakizawa
Takashi Kumagai
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Shinji Fukuda
Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
Frontiers in Microbiology
oral microbiome
oral metabolome
gut microbiome
gut metabolome
oral-gut axis
title Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
title_full Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
title_fullStr Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
title_full_unstemmed Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
title_short Integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth: a pilot study
title_sort integrated analysis of the oral and intestinal microbiome and metabolome of elderly people with more than 26 original teeth a pilot study
topic oral microbiome
oral metabolome
gut microbiome
gut metabolome
oral-gut axis
url https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233460/full
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