Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance
The most devastating abiotic factors worldwide are drought and salinity, causing severe bottlenecks in the agricultural sector. To acclimatize to these harsh ecological conditions, plants have developed complex molecular mechanisms involving diverse gene families. Among them, S-adenosyl-L-methionine...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/612 |
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| author | Joseph Wanjala Kilwake Muhammad Jawad Umer Yangyang Wei Teame Gereziher Mehari Richard Odongo Magwanga Yanchao Xu Yuqing Hou Yuhong Wang Margaret Linyerera Shiraku Joy Nyangasi Kirungu Xiaoyan Cai Zhongli Zhou Renhai Peng Fang Liu |
| author_facet | Joseph Wanjala Kilwake Muhammad Jawad Umer Yangyang Wei Teame Gereziher Mehari Richard Odongo Magwanga Yanchao Xu Yuqing Hou Yuhong Wang Margaret Linyerera Shiraku Joy Nyangasi Kirungu Xiaoyan Cai Zhongli Zhou Renhai Peng Fang Liu |
| author_sort | Joseph Wanjala Kilwake |
| collection | DOAJ |
| description | The most devastating abiotic factors worldwide are drought and salinity, causing severe bottlenecks in the agricultural sector. To acclimatize to these harsh ecological conditions, plants have developed complex molecular mechanisms involving diverse gene families. Among them, S-adenosyl-L-methionine synthetase (SAMS) genes initiate the physiological, morphological, and molecular changes to enable plants to adapt appropriately. We identified and characterized 16 upland cotton SAMS genes (<i>GhSAMSs</i>). Phylogenetic analysis classified the <i>GhSAMSs</i> into three major groups closely related to their homologs in soybean. Gene expression analysis under drought and salt stress conditions revealed that <i>GhSAMS2</i>, which has shown the highest interaction with <i>GhCBL10</i> (a key salt responsive gene), was the one that was most induced. <i>GhSAMS2</i> expression knockdown via virus-induced gene silencing (VGIS) enhanced transgenic plants’ susceptibility to drought and salt stress. The TRV2:<i>GhSAMS2</i> plants showed defects in terms of growth and physiological performances, including antioxidative processes, chlorophyll synthesis, and membrane permeability. Our findings provide insights into SAMS genes’ structure, classification, and role in abiotic stress response in upland cotton. Moreover, they show the potential of <i>GhSAMS2</i> for the targeted improvement of cotton plants’ tolerance to multiple abiotic stresses. |
| first_indexed | 2024-03-11T09:17:09Z |
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| id | doaj.art-983cba6dcde140a7a7bfaf11d5fb4975 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T09:17:09Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-983cba6dcde140a7a7bfaf11d5fb49752023-11-16T18:37:09ZengMDPI AGAgronomy2073-43952023-02-0113261210.3390/agronomy13020612Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress ToleranceJoseph Wanjala Kilwake0Muhammad Jawad Umer1Yangyang Wei2Teame Gereziher Mehari3Richard Odongo Magwanga4Yanchao Xu5Yuqing Hou6Yuhong Wang7Margaret Linyerera Shiraku8Joy Nyangasi Kirungu9Xiaoyan Cai10Zhongli Zhou11Renhai Peng12Fang Liu13State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaBiological and Food Engineering, Anyang Institute of Technology, Anyang 455000, ChinaSchool of Life Sciences, Nantong University, Nantong 226007, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaBiological and Food Engineering, Anyang Institute of Technology, Anyang 455000, ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang 455000, ChinaThe most devastating abiotic factors worldwide are drought and salinity, causing severe bottlenecks in the agricultural sector. To acclimatize to these harsh ecological conditions, plants have developed complex molecular mechanisms involving diverse gene families. Among them, S-adenosyl-L-methionine synthetase (SAMS) genes initiate the physiological, morphological, and molecular changes to enable plants to adapt appropriately. We identified and characterized 16 upland cotton SAMS genes (<i>GhSAMSs</i>). Phylogenetic analysis classified the <i>GhSAMSs</i> into three major groups closely related to their homologs in soybean. Gene expression analysis under drought and salt stress conditions revealed that <i>GhSAMS2</i>, which has shown the highest interaction with <i>GhCBL10</i> (a key salt responsive gene), was the one that was most induced. <i>GhSAMS2</i> expression knockdown via virus-induced gene silencing (VGIS) enhanced transgenic plants’ susceptibility to drought and salt stress. The TRV2:<i>GhSAMS2</i> plants showed defects in terms of growth and physiological performances, including antioxidative processes, chlorophyll synthesis, and membrane permeability. Our findings provide insights into SAMS genes’ structure, classification, and role in abiotic stress response in upland cotton. Moreover, they show the potential of <i>GhSAMS2</i> for the targeted improvement of cotton plants’ tolerance to multiple abiotic stresses.https://www.mdpi.com/2073-4395/13/2/612S-adenosyl-L-methionine synthetasevirus-induced gene silencing<i>SAMS2</i>abiotic stressupland cotton |
| spellingShingle | Joseph Wanjala Kilwake Muhammad Jawad Umer Yangyang Wei Teame Gereziher Mehari Richard Odongo Magwanga Yanchao Xu Yuqing Hou Yuhong Wang Margaret Linyerera Shiraku Joy Nyangasi Kirungu Xiaoyan Cai Zhongli Zhou Renhai Peng Fang Liu Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance Agronomy S-adenosyl-L-methionine synthetase virus-induced gene silencing <i>SAMS2</i> abiotic stress upland cotton |
| title | Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance |
| title_full | Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance |
| title_fullStr | Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance |
| title_full_unstemmed | Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance |
| title_short | Genome-Wide Characterization of the SAMS Gene Family in Cotton Unveils the Putative Role of <i>GhSAMS2</i> in Enhancing Abiotic Stress Tolerance |
| title_sort | genome wide characterization of the sams gene family in cotton unveils the putative role of i ghsams2 i in enhancing abiotic stress tolerance |
| topic | S-adenosyl-L-methionine synthetase virus-induced gene silencing <i>SAMS2</i> abiotic stress upland cotton |
| url | https://www.mdpi.com/2073-4395/13/2/612 |
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