Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury
Apple (Malus domestica, Borkh.) is one of the four largest fruits in the world. Freezing damage during the flowering period of apples is one of the main factors leading to the reduction or even extinction of apple production. Molecular breeding of hardy apples is a good solution to these problems. H...
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-0613 |
| _version_ | 1797368887257333760 |
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| author | Li Xiaolong Yue Haiying Chu Yannan Jia Yonghua |
| author_facet | Li Xiaolong Yue Haiying Chu Yannan Jia Yonghua |
| author_sort | Li Xiaolong |
| collection | DOAJ |
| description | Apple (Malus domestica, Borkh.) is one of the four largest fruits in the world. Freezing damage during the flowering period of apples is one of the main factors leading to the reduction or even extinction of apple production. Molecular breeding of hardy apples is a good solution to these problems. However, the current screening of cold tolerance genes still needs to be resolved. Therefore, in this article, the transcriptome detection and cold tolerance gene screening during the cold adaptation process of apple were studied in order to obtain potential cold-resistant genes. Herein, two high-quality apple tree species (Malus robusta Rehd and M. domestica) were used for cold adaptation experiments and studied under different low-temperature stress conditions (0, −2 and −4°C). The antioxidant levels of two apple flower tissues were tested, and the transcriptome of the flowers after cold culture was tested by next-generation sequencing technology. Antioxidant test results show that the elimination of peroxides in M. robusta Rehd and the adjustment of the expression of antioxidant enzymes promote the cold resistance of this variety of apples. Functional enrichment found that the expression of enzyme activity, cell wall and cell membrane structure, glucose metabolism/gluconeogenesis, and signal transmission are the main biological processes that affect the differences in the cold resistance characteristics of the two apples. In addition, three potential cold-resistant genes AtERF4, RuBisCO activase 1, and an unknown gene (ID: MD09G1075000) were screened. In this study, three potential cold-resistant genes (AtERF4, RuBisCO activase 1, and an unknown gene [ID: MD09G1075000]) and three cold-repressed differential genes (AtDTX29, XTH1, and TLP) were screened. |
| first_indexed | 2024-03-08T17:39:08Z |
| format | Article |
| id | doaj.art-983e96e7a6c84d19be8b73cbbe8f4291 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-08T17:39:08Z |
| publishDate | 2023-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-983e96e7a6c84d19be8b73cbbe8f42912024-01-02T11:33:22ZengDe GruyterOpen Life Sciences2391-54122023-12-011811272910.1515/biol-2022-0613Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injuryLi Xiaolong0Yue Haiying1Chu Yannan2Jia Yonghua3Department of Plant Science, Institute of Horticulture, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750000, Ningxia, ChinaDepartment of Plant Science, Institute of Horticulture, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750000, Ningxia, ChinaDepartment of Plant Science, Institute of Horticulture, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750000, Ningxia, ChinaDepartment of Plant Science, Institute of Horticulture, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750000, Ningxia, ChinaApple (Malus domestica, Borkh.) is one of the four largest fruits in the world. Freezing damage during the flowering period of apples is one of the main factors leading to the reduction or even extinction of apple production. Molecular breeding of hardy apples is a good solution to these problems. However, the current screening of cold tolerance genes still needs to be resolved. Therefore, in this article, the transcriptome detection and cold tolerance gene screening during the cold adaptation process of apple were studied in order to obtain potential cold-resistant genes. Herein, two high-quality apple tree species (Malus robusta Rehd and M. domestica) were used for cold adaptation experiments and studied under different low-temperature stress conditions (0, −2 and −4°C). The antioxidant levels of two apple flower tissues were tested, and the transcriptome of the flowers after cold culture was tested by next-generation sequencing technology. Antioxidant test results show that the elimination of peroxides in M. robusta Rehd and the adjustment of the expression of antioxidant enzymes promote the cold resistance of this variety of apples. Functional enrichment found that the expression of enzyme activity, cell wall and cell membrane structure, glucose metabolism/gluconeogenesis, and signal transmission are the main biological processes that affect the differences in the cold resistance characteristics of the two apples. In addition, three potential cold-resistant genes AtERF4, RuBisCO activase 1, and an unknown gene (ID: MD09G1075000) were screened. In this study, three potential cold-resistant genes (AtERF4, RuBisCO activase 1, and an unknown gene [ID: MD09G1075000]) and three cold-repressed differential genes (AtDTX29, XTH1, and TLP) were screened.https://doi.org/10.1515/biol-2022-0613applechilling genecold stresstranscriptomeanti-oxidation |
| spellingShingle | Li Xiaolong Yue Haiying Chu Yannan Jia Yonghua Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury Open Life Sciences apple chilling gene cold stress transcriptome anti-oxidation |
| title | Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| title_full | Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| title_fullStr | Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| title_full_unstemmed | Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| title_short | Comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| title_sort | comparative transcriptomes reveal molecular mechanisms of apple blossoms of different tolerance genotypes to chilling injury |
| topic | apple chilling gene cold stress transcriptome anti-oxidation |
| url | https://doi.org/10.1515/biol-2022-0613 |
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