Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine
Allantoin and its β-cyclodextrin and hydroxypropyl-β-cyclodextrin inclusion complexes 1:1 have been used to prepare carbopol-based mucoadhesive gels. The gelation process occurred by adjustment with glycerol 10% in the presence of triethanolamine. The structural features induced by the presence of a...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/7/416 |
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| author | Daniela Filip Doina Macocinschi Mirela-Fernanda Zaltariov Carmen Anatolia Gafitanu Cristina Gabriela Tuchilus Adrian Bele Bianca-Iulia Ciubotaru Elena Stoleru Alexandra Bargan |
| author_facet | Daniela Filip Doina Macocinschi Mirela-Fernanda Zaltariov Carmen Anatolia Gafitanu Cristina Gabriela Tuchilus Adrian Bele Bianca-Iulia Ciubotaru Elena Stoleru Alexandra Bargan |
| author_sort | Daniela Filip |
| collection | DOAJ |
| description | Allantoin and its β-cyclodextrin and hydroxypropyl-β-cyclodextrin inclusion complexes 1:1 have been used to prepare carbopol-based mucoadhesive gels. The gelation process occurred by adjustment with glycerol 10% in the presence of triethanolamine. The structural features induced by the presence of allantoin and the corresponding β-cyclodextrins inclusion complexes have been first investigated by infrared spectroscopy highlighting strong interactions within the gels network and ideal crosslinks for the self-healing behavior. The hydrophilicity of the gels was investigated by the determination of the surface tension parameters and the free energy of hydration. The interfacial free energy values indicated prolonged biocompatibility with blood. The gels-water molecule interactions in terms of sorption, permeability, and diffusion coefficients were evaluated by dynamic vapor sorption analysis. The self-assembly process through intermolecular H-bonding, the high hydrophilicity, the mechanical performance, the hydrolytic stability in simulated biological media, the biocompatibility with normal human dermal fibroblast (NHDF) cells, the mucoadhesivity and antimicrobial activity on selected microorganism species (<i>S. Aureus</i> and <i>C. albicans</i>) of the allantoin-based gels recommend them as promising scaffold alternatives in regenerative medicine. |
| first_indexed | 2024-03-09T03:23:40Z |
| format | Article |
| id | doaj.art-983ff95cbda546c1be7e5b0f54186f97 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T03:23:40Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-983ff95cbda546c1be7e5b0f54186f972023-12-03T15:05:09ZengMDPI AGGels2310-28612022-07-018741610.3390/gels8070416Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative MedicineDaniela Filip0Doina Macocinschi1Mirela-Fernanda Zaltariov2Carmen Anatolia Gafitanu3Cristina Gabriela Tuchilus4Adrian Bele5Bianca-Iulia Ciubotaru6Elena Stoleru7Alexandra Bargan8“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, RomaniaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania“Microbiology Department, Faculty of Medicine, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41 A, 700487 Iasi, RomaniaAllantoin and its β-cyclodextrin and hydroxypropyl-β-cyclodextrin inclusion complexes 1:1 have been used to prepare carbopol-based mucoadhesive gels. The gelation process occurred by adjustment with glycerol 10% in the presence of triethanolamine. The structural features induced by the presence of allantoin and the corresponding β-cyclodextrins inclusion complexes have been first investigated by infrared spectroscopy highlighting strong interactions within the gels network and ideal crosslinks for the self-healing behavior. The hydrophilicity of the gels was investigated by the determination of the surface tension parameters and the free energy of hydration. The interfacial free energy values indicated prolonged biocompatibility with blood. The gels-water molecule interactions in terms of sorption, permeability, and diffusion coefficients were evaluated by dynamic vapor sorption analysis. The self-assembly process through intermolecular H-bonding, the high hydrophilicity, the mechanical performance, the hydrolytic stability in simulated biological media, the biocompatibility with normal human dermal fibroblast (NHDF) cells, the mucoadhesivity and antimicrobial activity on selected microorganism species (<i>S. Aureus</i> and <i>C. albicans</i>) of the allantoin-based gels recommend them as promising scaffold alternatives in regenerative medicine.https://www.mdpi.com/2310-2861/8/7/416β-cyclodextrin/allantoin inclusion complexesinterfacial energymucoadhesionantibacterialtissue engineering scaffolds |
| spellingShingle | Daniela Filip Doina Macocinschi Mirela-Fernanda Zaltariov Carmen Anatolia Gafitanu Cristina Gabriela Tuchilus Adrian Bele Bianca-Iulia Ciubotaru Elena Stoleru Alexandra Bargan Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine Gels β-cyclodextrin/allantoin inclusion complexes interfacial energy mucoadhesion antibacterial tissue engineering scaffolds |
| title | Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine |
| title_full | Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine |
| title_fullStr | Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine |
| title_full_unstemmed | Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine |
| title_short | Mucoadhesive and Antimicrobial Allantoin/β Cyclodextrins-Loaded Carbopol Gels as Scaffolds for Regenerative Medicine |
| title_sort | mucoadhesive and antimicrobial allantoin β cyclodextrins loaded carbopol gels as scaffolds for regenerative medicine |
| topic | β-cyclodextrin/allantoin inclusion complexes interfacial energy mucoadhesion antibacterial tissue engineering scaffolds |
| url | https://www.mdpi.com/2310-2861/8/7/416 |
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