COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY
The aim of the study is to determine the effectiveness of the use of platelet enriched plasma in the complex treatment of chest trauma and chronic pleural empyema.Materials and methods. The work was performed on 450 male rats, simulated with chest trauma (n=180) and chronic pleural empyema (n=270)....
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| Format: | Article |
| Language: | Russian |
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Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute
2020-10-01
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| Series: | Фармация и фармакология (Пятигорск) |
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| Online Access: | https://www.pharmpharm.ru/jour/article/view/686 |
| _version_ | 1797874693025300480 |
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| author | Anton V. Kalashnikov Alexander A. Vorobiev Svetlana A. Kalashnikova Dmitry Sh. Salimov |
| author_facet | Anton V. Kalashnikov Alexander A. Vorobiev Svetlana A. Kalashnikova Dmitry Sh. Salimov |
| author_sort | Anton V. Kalashnikov |
| collection | DOAJ |
| description | The aim of the study is to determine the effectiveness of the use of platelet enriched plasma in the complex treatment of chest trauma and chronic pleural empyema.Materials and methods. The work was performed on 450 male rats, simulated with chest trauma (n=180) and chronic pleural empyema (n=270). In the experimental groups, biostimulation of adhesiogenesis as an intrapleural injection of 1 ml of platelet-enriched plasma was carried out; in the comparison group; the animals with pleural empyema were injected with 1 ml of doxycycline solution; in the negative control groups, the treatment was not carried out at all. Withdrawal from the experiment took place on the 10th, 20th, 30th days. The samples of intrapleural adhesions were fixed in 10% formalin, followed by histological tracing and preparation of micropreparations, staining with hematoxylin and eosin. The morphometric study included determination of the volume fraction (VF) of collagen and reticular fibers; fibrin; inflammatory cells; blood-stream (%).Results. An intrapleural administration of platelet-rich plasma is an effective way to stabilize the rib cage in chest injuries, and to eliminate residual cavities in chronic pleural empyema. When assessing the severity of the adhesions in chest trauma, it was found out that adhesions are most often visualized at the sites of rib fractures (from 13.3 to 40%). In pleural empyema, during the entire process of observation, the VF of collagen fibers forming adhesions was higher in the group with biological stimulation of adhesiogenesis than in the NCpe group and in the CG. In the PRP group, already at the initial stages of the experiment, this indicator was significantly lower than in the NC and CG (p<0.05).Conclusion. Based on the data obtained, the effectiveness of the use of platelet-enriched plasma in thoracic surgery for the biological potentiation of adhesiogenesis has been proved in experimental chest injuries and chronic pleural empyema. |
| first_indexed | 2024-04-10T01:36:06Z |
| format | Article |
| id | doaj.art-9840abd7cc74431bbf0fddbf6d292445 |
| institution | Directory Open Access Journal |
| issn | 2307-9266 2413-2241 |
| language | Russian |
| last_indexed | 2024-04-10T01:36:06Z |
| publishDate | 2020-10-01 |
| publisher | Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute |
| record_format | Article |
| series | Фармация и фармакология (Пятигорск) |
| spelling | doaj.art-9840abd7cc74431bbf0fddbf6d2924452023-03-13T09:18:12ZrusVolgograd State Medical University, Pyatigorsk Medical and Pharmaceutical InstituteФармация и фармакология (Пятигорск)2307-92662413-22412020-10-0182869910.19163/2307-9266-2020-8-2-86-99336COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERYAnton V. Kalashnikov0Alexander A. Vorobiev1Svetlana A. Kalashnikova2Dmitry Sh. Salimov3Пятигорский медико-фармацевтический институт - филиал Волгоградского государственного медицинского университетаВолгоградский государственный медицинский университетПятигорский медико-фармацевтический институт - филиал Волгоградского государственного медицинского университетаЦентральный военный клинический госпиталь имени П.В. МандрыкаThe aim of the study is to determine the effectiveness of the use of platelet enriched plasma in the complex treatment of chest trauma and chronic pleural empyema.Materials and methods. The work was performed on 450 male rats, simulated with chest trauma (n=180) and chronic pleural empyema (n=270). In the experimental groups, biostimulation of adhesiogenesis as an intrapleural injection of 1 ml of platelet-enriched plasma was carried out; in the comparison group; the animals with pleural empyema were injected with 1 ml of doxycycline solution; in the negative control groups, the treatment was not carried out at all. Withdrawal from the experiment took place on the 10th, 20th, 30th days. The samples of intrapleural adhesions were fixed in 10% formalin, followed by histological tracing and preparation of micropreparations, staining with hematoxylin and eosin. The morphometric study included determination of the volume fraction (VF) of collagen and reticular fibers; fibrin; inflammatory cells; blood-stream (%).Results. An intrapleural administration of platelet-rich plasma is an effective way to stabilize the rib cage in chest injuries, and to eliminate residual cavities in chronic pleural empyema. When assessing the severity of the adhesions in chest trauma, it was found out that adhesions are most often visualized at the sites of rib fractures (from 13.3 to 40%). In pleural empyema, during the entire process of observation, the VF of collagen fibers forming adhesions was higher in the group with biological stimulation of adhesiogenesis than in the NCpe group and in the CG. In the PRP group, already at the initial stages of the experiment, this indicator was significantly lower than in the NC and CG (p<0.05).Conclusion. Based on the data obtained, the effectiveness of the use of platelet-enriched plasma in thoracic surgery for the biological potentiation of adhesiogenesis has been proved in experimental chest injuries and chronic pleural empyema.https://www.pharmpharm.ru/jour/article/view/686торакальная хирургиябиостимуляция адгезиогенезаплазмаобогащенная тромбоцитамитравма грудной клеткиэмпиема плевры |
| spellingShingle | Anton V. Kalashnikov Alexander A. Vorobiev Svetlana A. Kalashnikova Dmitry Sh. Salimov COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY Фармация и фармакология (Пятигорск) торакальная хирургия биостимуляция адгезиогенеза плазма обогащенная тромбоцитами травма грудной клетки эмпиема плевры |
| title | COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY |
| title_full | COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY |
| title_fullStr | COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY |
| title_full_unstemmed | COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY |
| title_short | COMPLEX BIOSTIMULATION OF INTRAPLEURAL ADHESIOGENESIS IN THORACAL SURGERY |
| title_sort | complex biostimulation of intrapleural adhesiogenesis in thoracal surgery |
| topic | торакальная хирургия биостимуляция адгезиогенеза плазма обогащенная тромбоцитами травма грудной клетки эмпиема плевры |
| url | https://www.pharmpharm.ru/jour/article/view/686 |
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