Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens
Background: Coccidiosis is a serious protozoal disease of poultry. The iden-tification of Eimeria species has important implications for diagnosis and con-trol as well as for epidemiology. The molecular characterization of Eimeria spe-cies infecting Egyptian baladi chickens was investigated. Met...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/348 |
| _version_ | 1798029320449425408 |
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| author | Sahar M Gadelhaq Waleed M Arafa Shawky M Aboelhadid |
| author_facet | Sahar M Gadelhaq Waleed M Arafa Shawky M Aboelhadid |
| author_sort | Sahar M Gadelhaq |
| collection | DOAJ |
| description | Background: Coccidiosis is a serious protozoal disease of poultry. The iden-tification of Eimeria species has important implications for diagnosis and con-trol as well as for epidemiology. The molecular characterization of Eimeria spe-cies infecting Egyptian baladi chickens was investigated.
Methods:Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted ini-tially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conven-tional polymerase chain reaction by using amplified region (SCAR) marker.
Results:The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. max-ima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima. Analysis of the obtained sequences revealed high identi-ties 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence.
Conclusion:This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens. |
| first_indexed | 2024-04-11T19:22:27Z |
| format | Article |
| id | doaj.art-98412334a88045de9824b2bd8f781316 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T19:22:27Z |
| publishDate | 2015-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-98412334a88045de9824b2bd8f7813162022-12-22T04:07:16ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2015-03-01101327Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi ChickensSahar M Gadelhaq0Waleed M Arafa1Shawky M Aboelhadid2Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.Background: Coccidiosis is a serious protozoal disease of poultry. The iden-tification of Eimeria species has important implications for diagnosis and con-trol as well as for epidemiology. The molecular characterization of Eimeria spe-cies infecting Egyptian baladi chickens was investigated. Methods:Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted ini-tially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conven-tional polymerase chain reaction by using amplified region (SCAR) marker. Results:The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. max-ima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima. Analysis of the obtained sequences revealed high identi-ties 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence. Conclusion:This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/348ChickensDNAEimeria speciesNucleotidePCRSequencing |
| spellingShingle | Sahar M Gadelhaq Waleed M Arafa Shawky M Aboelhadid Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens Iranian Journal of Parasitology Chickens DNA Eimeria species Nucleotide PCR Sequencing |
| title | Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens |
| title_full | Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens |
| title_fullStr | Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens |
| title_full_unstemmed | Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens |
| title_short | Molecular Characterization of Eimeria Species Naturally Infecting Egyptian Baldi Chickens |
| title_sort | molecular characterization of eimeria species naturally infecting egyptian baldi chickens |
| topic | Chickens DNA Eimeria species Nucleotide PCR Sequencing |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/348 |
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