Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating
Calcium phosphate-based coatings are widely studied in orthopedics and dentistry for their similarity to the mineral component of bone and their capability to promote osseointegration. Different calcium phosphates have tunable properties that result in different behaviors in vitro, but the majority...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/11/1758 |
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| author | Matteo Montesissa Giorgia Borciani Katia Rubini Francesco Valle Marco Boi Nicola Baldini Elisa Boanini Gabriela Graziani |
| author_facet | Matteo Montesissa Giorgia Borciani Katia Rubini Francesco Valle Marco Boi Nicola Baldini Elisa Boanini Gabriela Graziani |
| author_sort | Matteo Montesissa |
| collection | DOAJ |
| description | Calcium phosphate-based coatings are widely studied in orthopedics and dentistry for their similarity to the mineral component of bone and their capability to promote osseointegration. Different calcium phosphates have tunable properties that result in different behaviors in vitro, but the majority of studies focus only on hydroxyapatite. Here, different calcium phosphate-based nanostructured coatings are obtained by ionized jet deposition, starting with hydroxyapatite, brushite and beta-tricalcium phosphate targets. The properties of the coatings obtained from different precursors are systematically compared by assessing their composition, morphology, physical and mechanical properties, dissolution, and in vitro behavior. In addition, for the first time, depositions at high temperature are investigated for the further tuning of the coatings mechanical properties and stability. Results show that different phosphates can be deposited with good composition fidelity even if not in a crystalline phase. All coatings are nanostructured and non-cytotoxic and display variable surface roughness and wettability. Upon heating, higher adhesion and hydrophilicity are obtained as well as higher stability, resulting in better cell viability. Interestingly, different phosphates show very different in vitro behavior, with brushite being the most suitable for promoting cell viability and beta-tricalcium phosphate having a higher impact on cell morphology at the early timepoints. |
| first_indexed | 2024-03-11T03:00:52Z |
| format | Article |
| id | doaj.art-98416ef3fbb349488535b7e36f51c1ce |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T03:00:52Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-98416ef3fbb349488535b7e36f51c1ce2023-11-18T08:19:07ZengMDPI AGNanomaterials2079-49912023-05-011311175810.3390/nano13111758Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate HeatingMatteo Montesissa0Giorgia Borciani1Katia Rubini2Francesco Valle3Marco Boi4Nicola Baldini5Elisa Boanini6Gabriela Graziani7Department of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, University of Bologna, 40126 Bologna, ItalyInstitute of Nanostructured Materials, National Research Council, 40129 Bologna, ItalyBST Biomedical Science and Technologies and Nanobiotechnology Laboratory, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, University of Bologna, 40126 Bologna, ItalyBST Biomedical Science and Technologies and Nanobiotechnology Laboratory, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyCalcium phosphate-based coatings are widely studied in orthopedics and dentistry for their similarity to the mineral component of bone and their capability to promote osseointegration. Different calcium phosphates have tunable properties that result in different behaviors in vitro, but the majority of studies focus only on hydroxyapatite. Here, different calcium phosphate-based nanostructured coatings are obtained by ionized jet deposition, starting with hydroxyapatite, brushite and beta-tricalcium phosphate targets. The properties of the coatings obtained from different precursors are systematically compared by assessing their composition, morphology, physical and mechanical properties, dissolution, and in vitro behavior. In addition, for the first time, depositions at high temperature are investigated for the further tuning of the coatings mechanical properties and stability. Results show that different phosphates can be deposited with good composition fidelity even if not in a crystalline phase. All coatings are nanostructured and non-cytotoxic and display variable surface roughness and wettability. Upon heating, higher adhesion and hydrophilicity are obtained as well as higher stability, resulting in better cell viability. Interestingly, different phosphates show very different in vitro behavior, with brushite being the most suitable for promoting cell viability and beta-tricalcium phosphate having a higher impact on cell morphology at the early timepoints.https://www.mdpi.com/2079-4991/13/11/1758coatingsorthopedicsdentistrycalcium phosphatesionized jet depositionhydroxyapatite |
| spellingShingle | Matteo Montesissa Giorgia Borciani Katia Rubini Francesco Valle Marco Boi Nicola Baldini Elisa Boanini Gabriela Graziani Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating Nanomaterials coatings orthopedics dentistry calcium phosphates ionized jet deposition hydroxyapatite |
| title | Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating |
| title_full | Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating |
| title_fullStr | Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating |
| title_full_unstemmed | Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating |
| title_short | Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating |
| title_sort | ionized jet deposition of calcium phosphates based nanocoatings tuning coating properties and cell behavior by target composition and substrate heating |
| topic | coatings orthopedics dentistry calcium phosphates ionized jet deposition hydroxyapatite |
| url | https://www.mdpi.com/2079-4991/13/11/1758 |
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