Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line
<b>Background:</b> This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. <b>Methodology:</b> Nutritional content of all tea plants were determined...
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/11/3505 |
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| author | Motlalepula Gilbert Matsabisa Asis Bala Satyajit Tripathy Michelle Mogomane Digashu Fanie Rautenbach Barsha Dassarma Joseph Omorogiuwa Erhabor Fernao Castro Braga Pulok Kumar Mukherjee Minke Tang Youngmin Kang |
| author_facet | Motlalepula Gilbert Matsabisa Asis Bala Satyajit Tripathy Michelle Mogomane Digashu Fanie Rautenbach Barsha Dassarma Joseph Omorogiuwa Erhabor Fernao Castro Braga Pulok Kumar Mukherjee Minke Tang Youngmin Kang |
| author_sort | Motlalepula Gilbert Matsabisa |
| collection | DOAJ |
| description | <b>Background:</b> This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. <b>Methodology:</b> Nutritional content of all tea plants were determined for energy, fat, carbohydrates, total sugars, dietary fiber and amino acids. Antioxidant potential and the antioxidant potentiating secondary metabolites were also measured and compared. Further, we investigated the tea plants for any role they would have on cyclooxygenase (COX) activity on cobalt chloride (CoCl<sub>2</sub>) induced human glioma cell lines (U87MG). <b>Results:</b> The tea plants were found non-cytotoxic at concentrations tested against the human Chang liver and HeK 293 kidney cells and were found to be naturally caffeine free. The lowest and highest extraction yield among the tea plants was 7.1% for <i>B. saligna</i> and 15.48% for <i>L. scaberrimma</i> respectively. On average, the flavonol content was 12 to 8 QE/g, ORAC 800 µmol TE/g, TEAC 150 µmol TE/g, FRAP 155 µmol AAE/g, polyphenols 40 mg GAE/g, flavanols 0.35 mg CE/g, flavonols 12 mg QE/g and total flavonoid content (TFC) 180 µg QE/mg. The COX activity has been found to be inhibited by a dose-dependent manner by <i>L. scaberrimma</i>, <i>B. saligna</i> and <i>L. javanica</i>. <b>Conclusion:</b> The results further support competitive value of tea plants and need for improved and further development. |
| first_indexed | 2024-03-10T01:04:40Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:04:40Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9843b69fc3dd47559b301f6131adc3582023-11-23T14:29:49ZengMDPI AGMolecules1420-30492022-05-012711350510.3390/molecules27113505Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell LineMotlalepula Gilbert Matsabisa0Asis Bala1Satyajit Tripathy2Michelle Mogomane Digashu3Fanie Rautenbach4Barsha Dassarma5Joseph Omorogiuwa Erhabor6Fernao Castro Braga7Pulok Kumar Mukherjee8Minke Tang9Youngmin Kang10Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaDepartment of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaDepartment of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaDepartment of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaFaculty of Health and Wellness Sciences, Oxidative Research Institute, Cape Peninsula University of Technology, Cape Town 7530, South AfricaDepartment of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaDepartment of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Free State, Bloemfontein 9300, South AfricaFaculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilSchool of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, IndiaDepartment of Pharmacology, School of Pharmacy, Beijing University of Chinese Medicines, Beijing 100191, ChinaPropagation and Production of Traditional Herbal Medicines, Herbal Medicines Resources Centre, Korean Institute of Oriental Medicine, University of Science and Technology, Daejeon 34054, Korea<b>Background:</b> This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. <b>Methodology:</b> Nutritional content of all tea plants were determined for energy, fat, carbohydrates, total sugars, dietary fiber and amino acids. Antioxidant potential and the antioxidant potentiating secondary metabolites were also measured and compared. Further, we investigated the tea plants for any role they would have on cyclooxygenase (COX) activity on cobalt chloride (CoCl<sub>2</sub>) induced human glioma cell lines (U87MG). <b>Results:</b> The tea plants were found non-cytotoxic at concentrations tested against the human Chang liver and HeK 293 kidney cells and were found to be naturally caffeine free. The lowest and highest extraction yield among the tea plants was 7.1% for <i>B. saligna</i> and 15.48% for <i>L. scaberrimma</i> respectively. On average, the flavonol content was 12 to 8 QE/g, ORAC 800 µmol TE/g, TEAC 150 µmol TE/g, FRAP 155 µmol AAE/g, polyphenols 40 mg GAE/g, flavanols 0.35 mg CE/g, flavonols 12 mg QE/g and total flavonoid content (TFC) 180 µg QE/mg. The COX activity has been found to be inhibited by a dose-dependent manner by <i>L. scaberrimma</i>, <i>B. saligna</i> and <i>L. javanica</i>. <b>Conclusion:</b> The results further support competitive value of tea plants and need for improved and further development.https://www.mdpi.com/1420-3049/27/11/3505indigenous teacommercial teaantioxidant capacitynutritional contentcox activity |
| spellingShingle | Motlalepula Gilbert Matsabisa Asis Bala Satyajit Tripathy Michelle Mogomane Digashu Fanie Rautenbach Barsha Dassarma Joseph Omorogiuwa Erhabor Fernao Castro Braga Pulok Kumar Mukherjee Minke Tang Youngmin Kang Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line Molecules indigenous tea commercial tea antioxidant capacity nutritional content cox activity |
| title | Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line |
| title_full | Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line |
| title_fullStr | Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line |
| title_full_unstemmed | Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line |
| title_short | Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line |
| title_sort | study on south african indigenous teas antioxidant potential nutritional content and hypoxia induced cyclooxygenase inhibition on u87 mg cell line |
| topic | indigenous tea commercial tea antioxidant capacity nutritional content cox activity |
| url | https://www.mdpi.com/1420-3049/27/11/3505 |
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