In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur.
In folk medicine, <i>Ficus tikoua</i> (<i>F. tikoua</i>) has been used to treat diabetes for a long time, but there is a rare modern pharmacological investigation for its antidiabetic effect and mechanisms. Our study aimed to evaluate its hypoglycemic effect using in vitro an...
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MDPI AG
2022-10-01
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| Series: | Nutrients |
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| author | Hanlei Wang Kun Zhang Xuelin Chen Mei Han Jing Lu Yumei Zhang |
| author_facet | Hanlei Wang Kun Zhang Xuelin Chen Mei Han Jing Lu Yumei Zhang |
| author_sort | Hanlei Wang |
| collection | DOAJ |
| description | In folk medicine, <i>Ficus tikoua</i> (<i>F. tikoua</i>) has been used to treat diabetes for a long time, but there is a rare modern pharmacological investigation for its antidiabetic effect and mechanisms. Our study aimed to evaluate its hypoglycemic effect using in vitro and in vivo experimental models and then explore the possible mechanisms. In the ethanol extracts and fractions of <i>F. tikoua</i>, n-butanol fraction (NBF) exhibited the most potent effect on inhibiting <i>α</i>-glucosidase activity (IC<sub>50</sub> = 0.89 ± 0.04 μg/mL) and promoting glucose uptake in 3T3-L1 adipocytes. Further animal experiments showed that NBF could play an antidiabetic role by ameliorating random blood glucose, fasting blood glucose, oral glucose tolerance, HbA1c level, and islets damage in diabetic mice. Then, the activities of the five subfractions of NBF (NBF1-NBF5) were further evaluated; NBF2 showed stronger <i>α</i>-glucosidase inhibition activities (IC<sub>50</sub> = 0.32 ± 0.05 μg/mL) than NBF. Moreover, NBF2 also possessed the ability to promote glucose uptake, which was mediated via P13K/AKT and AMPK pathways. This study demonstrated that <i>F. tikoua</i> possesses antidiabetic efficacy in vitro and in vivo and provided a scientific basis for its folk medicinal use. NBF2 might be potential natural candidate drugs to treat diabetes mellitus. It is the first time the antidiabetic activity and the potential mechanisms of NBF2 were reported. |
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| spelling | doaj.art-98456fe4e9304cac9a6e65f9389a47c62023-11-24T01:46:26ZengMDPI AGNutrients2072-66432022-10-011420441310.3390/nu14204413In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur.Hanlei Wang0Kun Zhang1Xuelin Chen2Mei Han3Jing Lu4Yumei Zhang5Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaKey Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaKey Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaKey Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaKey Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaKey Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88 Xuefu Road, Kunming 650223, ChinaIn folk medicine, <i>Ficus tikoua</i> (<i>F. tikoua</i>) has been used to treat diabetes for a long time, but there is a rare modern pharmacological investigation for its antidiabetic effect and mechanisms. Our study aimed to evaluate its hypoglycemic effect using in vitro and in vivo experimental models and then explore the possible mechanisms. In the ethanol extracts and fractions of <i>F. tikoua</i>, n-butanol fraction (NBF) exhibited the most potent effect on inhibiting <i>α</i>-glucosidase activity (IC<sub>50</sub> = 0.89 ± 0.04 μg/mL) and promoting glucose uptake in 3T3-L1 adipocytes. Further animal experiments showed that NBF could play an antidiabetic role by ameliorating random blood glucose, fasting blood glucose, oral glucose tolerance, HbA1c level, and islets damage in diabetic mice. Then, the activities of the five subfractions of NBF (NBF1-NBF5) were further evaluated; NBF2 showed stronger <i>α</i>-glucosidase inhibition activities (IC<sub>50</sub> = 0.32 ± 0.05 μg/mL) than NBF. Moreover, NBF2 also possessed the ability to promote glucose uptake, which was mediated via P13K/AKT and AMPK pathways. This study demonstrated that <i>F. tikoua</i> possesses antidiabetic efficacy in vitro and in vivo and provided a scientific basis for its folk medicinal use. NBF2 might be potential natural candidate drugs to treat diabetes mellitus. It is the first time the antidiabetic activity and the potential mechanisms of NBF2 were reported.https://www.mdpi.com/2072-6643/14/20/4413<i>Ficus tikoua</i> Bur.diabetes mellitus<i>α</i>-glucosidase3T3-L1 adipocytes<i>db/db</i> mice |
| spellingShingle | Hanlei Wang Kun Zhang Xuelin Chen Mei Han Jing Lu Yumei Zhang In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. Nutrients <i>Ficus tikoua</i> Bur. diabetes mellitus <i>α</i>-glucosidase 3T3-L1 adipocytes <i>db/db</i> mice |
| title | In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. |
| title_full | In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. |
| title_fullStr | In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. |
| title_full_unstemmed | In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. |
| title_short | In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of <i>Ficus tikoua</i> Bur. |
| title_sort | in vitro and in vivo evaluation of antidiabetic properties and mechanisms of i ficus tikoua i bur |
| topic | <i>Ficus tikoua</i> Bur. diabetes mellitus <i>α</i>-glucosidase 3T3-L1 adipocytes <i>db/db</i> mice |
| url | https://www.mdpi.com/2072-6643/14/20/4413 |
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