An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation.
BACKGROUND: Integrins are a family of adhesion receptors which regulate cell proliferation, differentiation, leukocyte migration, and complement receptor-dependent phagocytosis. In invertebrates, as a cell adhesion receptor, β integrins play an important role for the balanced activation of immune de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3392225?pdf=render |
| _version_ | 1811219981361217536 |
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| author | Ying Zhang Leilei Wang Lingling Wang Ning Wu Zhi Zhou Linsheng Song |
| author_facet | Ying Zhang Leilei Wang Lingling Wang Ning Wu Zhi Zhou Linsheng Song |
| author_sort | Ying Zhang |
| collection | DOAJ |
| description | BACKGROUND: Integrins are a family of adhesion receptors which regulate cell proliferation, differentiation, leukocyte migration, and complement receptor-dependent phagocytosis. In invertebrates, as a cell adhesion receptor, β integrins play an important role for the balanced activation of immune defense responses especially during the encounter of infections. The present study attempts to characterize the immune functions of shrimp integrin (LvIntegrin) to have better understanding on the immune system and its regulation mechanisms in shrimps. METHODOLOGY: A shrimp integrin was identified from the Pacific white shrimp Litopenaeus vannamei (designated as LvIntegrin). Its full-length cDNA was of 2621 bp with an open reading frame (ORF) of 2439 bp encoding a polypeptide of 812 amino acids. The mRNA expression of LvIntegrin was significantly up-regulated at 3, 6 and 12 h after Listonella anguillarum challenge. The cDNA fragment encoding β integrin domains (βA and hybrid domain) of LvIntegrin was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvIntegrin) could significantly agglutinate the tested microbe including E. coli JM109, L. anguillarum, Micrococcus luteus and Candida dattiladattila in the presence of divalent cations. Moreover, when NIH3T3 cells were cultured with rLvIntegrin, the proliferation rate increased significantly in a dose-dependent manner. CONCLUSIONS: LvIntegrin, a shrimp β integrin was identified from L. vannamei, shared several highly conserved features. LvIntegrin exhibited broad-spectrum agglutination activity towards both bacteria and fungi and could improve the proliferation of NIH3T3 cells, indicating that LvIntegrin is involved in the immune response against microbe challenge and regulation of cell proliferation as a cell adhesion receptor in shrimp. |
| first_indexed | 2024-04-12T07:34:48Z |
| format | Article |
| id | doaj.art-9846465d328a4309a370d4ac5740b437 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:34:48Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-9846465d328a4309a370d4ac5740b4372022-12-22T03:41:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4061510.1371/journal.pone.0040615An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation.Ying ZhangLeilei WangLingling WangNing WuZhi ZhouLinsheng SongBACKGROUND: Integrins are a family of adhesion receptors which regulate cell proliferation, differentiation, leukocyte migration, and complement receptor-dependent phagocytosis. In invertebrates, as a cell adhesion receptor, β integrins play an important role for the balanced activation of immune defense responses especially during the encounter of infections. The present study attempts to characterize the immune functions of shrimp integrin (LvIntegrin) to have better understanding on the immune system and its regulation mechanisms in shrimps. METHODOLOGY: A shrimp integrin was identified from the Pacific white shrimp Litopenaeus vannamei (designated as LvIntegrin). Its full-length cDNA was of 2621 bp with an open reading frame (ORF) of 2439 bp encoding a polypeptide of 812 amino acids. The mRNA expression of LvIntegrin was significantly up-regulated at 3, 6 and 12 h after Listonella anguillarum challenge. The cDNA fragment encoding β integrin domains (βA and hybrid domain) of LvIntegrin was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvIntegrin) could significantly agglutinate the tested microbe including E. coli JM109, L. anguillarum, Micrococcus luteus and Candida dattiladattila in the presence of divalent cations. Moreover, when NIH3T3 cells were cultured with rLvIntegrin, the proliferation rate increased significantly in a dose-dependent manner. CONCLUSIONS: LvIntegrin, a shrimp β integrin was identified from L. vannamei, shared several highly conserved features. LvIntegrin exhibited broad-spectrum agglutination activity towards both bacteria and fungi and could improve the proliferation of NIH3T3 cells, indicating that LvIntegrin is involved in the immune response against microbe challenge and regulation of cell proliferation as a cell adhesion receptor in shrimp.http://europepmc.org/articles/PMC3392225?pdf=render |
| spellingShingle | Ying Zhang Leilei Wang Lingling Wang Ning Wu Zhi Zhou Linsheng Song An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. PLoS ONE |
| title | An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. |
| title_full | An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. |
| title_fullStr | An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. |
| title_full_unstemmed | An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. |
| title_short | An integrin from shrimp Litopenaeus vannamei mediated microbial agglutination and cell proliferation. |
| title_sort | integrin from shrimp litopenaeus vannamei mediated microbial agglutination and cell proliferation |
| url | http://europepmc.org/articles/PMC3392225?pdf=render |
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